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A Laterally Acquired Galactose Oxidase-Like Gene Is Required for Aerial Development during Osmotic Stress in Streptomyces coelicolor / Recep Liman; Paul D Facey; Geertje Van Keulen; Paul J Dyson; Ricardo del Sol

PLoS ONE, Volume: 8, Issue: 1, Start page: e54112

Swansea University Author: Van Keulen, Geertje

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Abstract

Phylogenetic reconstruction revealed that most Actinobacterial orthologs of S. coelicolor SCO2837, encoding a metal-dependent galactose oxidase-like protein, are found within Streptomyces and were probably acquired by horizontal gene transfer from fungi. Disruption of SCO2837 (glxA) caused a conditi...

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Published in: PLoS ONE
ISSN: 1932-6203
Published: 2013
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URI: https://cronfa.swan.ac.uk/Record/cronfa14136
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first_indexed 2013-07-23T12:11:48Z
last_indexed 2018-02-09T04:45:21Z
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spelling 2013-09-23T11:03:33Z v2 14136 2013-01-30 A Laterally Acquired Galactose Oxidase-Like Gene Is Required for Aerial Development during Osmotic Stress in Streptomyces coelicolor Geertje Van Keulen Geertje Van Keulen true 0000-0002-6044-1575 false 6b2c798924ac19de63e2168d50b99425 9a9b1f5c7e5730932420ba55c1b56679 TMTpzy2phXvI6/CEdMyl7X2HZhUyFASdV1DFdgIIhKs= 2013-01-30 PMSC Phylogenetic reconstruction revealed that most Actinobacterial orthologs of S. coelicolor SCO2837, encoding a metal-dependent galactose oxidase-like protein, are found within Streptomyces and were probably acquired by horizontal gene transfer from fungi. Disruption of SCO2837 (glxA) caused a conditional bld phenotype that could not be reversed by extracellular complementation. Studies aimed at characterising the regulation of expression of glxA showed that it is not a target for other bld genes. We provide evidence that glxA is required for osmotic adaptation, although independently from the known osmotic stress response element SigB. glxA has been predicted to be part of an operon with the transcription unit comprising the upstream cslA gene and glxA. However, both phenotypic and expression studies indicate that it is also expressed from an independent promoter region internal to cslA. GlxA displays an in situ localisation pattern similar to that one observed for CslA at hyphal tips, but localisation of the former is independent of the latter. The functional role of GlxA in relation to CslA is discussed. Journal article PLoS ONE 8 1 e54112 1932-6203 Streptomyces; galactose oxidase; bald; horizontal gene transfer 11 1 2013 2013-01-11 10.1371/journal.pone.0054112 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0054112 - Swansea University Medical School Medicine CMED PMSC None 2013-09-23T11:03:33Z 2013-01-30T10:28:55Z Swansea University Medical School Medicine Recep Liman 1 Paul D Facey 2 Geertje Van Keulen 3 Paul J Dyson 4 Ricardo del Sol 5
title A Laterally Acquired Galactose Oxidase-Like Gene Is Required for Aerial Development during Osmotic Stress in Streptomyces coelicolor
spellingShingle A Laterally Acquired Galactose Oxidase-Like Gene Is Required for Aerial Development during Osmotic Stress in Streptomyces coelicolor
Van Keulen, Geertje
title_short A Laterally Acquired Galactose Oxidase-Like Gene Is Required for Aerial Development during Osmotic Stress in Streptomyces coelicolor
title_full A Laterally Acquired Galactose Oxidase-Like Gene Is Required for Aerial Development during Osmotic Stress in Streptomyces coelicolor
title_fullStr A Laterally Acquired Galactose Oxidase-Like Gene Is Required for Aerial Development during Osmotic Stress in Streptomyces coelicolor
title_full_unstemmed A Laterally Acquired Galactose Oxidase-Like Gene Is Required for Aerial Development during Osmotic Stress in Streptomyces coelicolor
title_sort A Laterally Acquired Galactose Oxidase-Like Gene Is Required for Aerial Development during Osmotic Stress in Streptomyces coelicolor
author_id_str_mv 6b2c798924ac19de63e2168d50b99425
author_id_fullname_str_mv 6b2c798924ac19de63e2168d50b99425_***_Van Keulen, Geertje
author Van Keulen, Geertje
author2 Recep Liman
Paul D Facey
Geertje Van Keulen
Paul J Dyson
Ricardo del Sol
format Journal article
container_title PLoS ONE
container_volume 8
container_issue 1
container_start_page e54112
publishDate 2013
institution Swansea University
issn 1932-6203
doi_str_mv 10.1371/journal.pone.0054112
college_str Swansea University Medical School
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hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
url http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0054112
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description Phylogenetic reconstruction revealed that most Actinobacterial orthologs of S. coelicolor SCO2837, encoding a metal-dependent galactose oxidase-like protein, are found within Streptomyces and were probably acquired by horizontal gene transfer from fungi. Disruption of SCO2837 (glxA) caused a conditional bld phenotype that could not be reversed by extracellular complementation. Studies aimed at characterising the regulation of expression of glxA showed that it is not a target for other bld genes. We provide evidence that glxA is required for osmotic adaptation, although independently from the known osmotic stress response element SigB. glxA has been predicted to be part of an operon with the transcription unit comprising the upstream cslA gene and glxA. However, both phenotypic and expression studies indicate that it is also expressed from an independent promoter region internal to cslA. GlxA displays an in situ localisation pattern similar to that one observed for CslA at hyphal tips, but localisation of the former is independent of the latter. The functional role of GlxA in relation to CslA is discussed.
published_date 2013-01-11T12:30:30Z
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