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The CentriMag Centrifugal Blood Pump as a Benchmark for In Vitro Testing of Hemocompatibility in Implantable Ventricular Assist Devices / Chris H.H. Chan; Ina Laura Pieper; Rebecca Hambly; Gemma Radley; Alyssa Jones; Yasmin Friedmann; Karl M. Hawkins; Stephen Westaby; Graham Foster; Catherine A. Thornton

Artificial Organs, Volume: 39, Issue: 2, Pages: 93 - 101

Swansea University Author: Thornton, Catherine

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DOI (Published version): 10.1111/aor.12351

Abstract

Implantable ventricular assist devices (VADs) have proven efficient in advanced heart failure patients as a bridge-to-transplant or destination therapy. However, VAD usage often leads to infection, bleeding and thrombosis, side effects attributable to the damage to blood cells and plasma proteins. W...

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Published in: Artificial Organs
Published: 2014
URI: https://cronfa.swan.ac.uk/Record/cronfa18309
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spelling 2016-04-20T14:17:54Z v2 18309 2014-09-02 The CentriMag Centrifugal Blood Pump as a Benchmark for In Vitro Testing of Hemocompatibility in Implantable Ventricular Assist Devices Catherine Thornton Catherine Thornton true 0000-0002-5153-573X false c71a7a4be7361094d046d312202bce0c 0789291f2bbb8aeb948db2352e425e6a 70js5AkMS5jPo3arCUY9c32HZhUyFASdV1DFdgIIhKs= 2014-09-02 BMS Implantable ventricular assist devices (VADs) have proven efficient in advanced heart failure patients as a bridge-to-transplant or destination therapy. However, VAD usage often leads to infection, bleeding and thrombosis, side effects attributable to the damage to blood cells and plasma proteins. We have shown previously that m Measuring haemolysis alone does not provide sufficient information to understand total blood damage, and research exploring the impact of currently available pumps on a wider range of blood cell types and plasma proteins such as von Willebrand factor (vWF) within blood is required to further our understanding of safer pump design. The extracorporeal CentriMag® has a haemolysis profile within published standards for haemolysis of NIH levels of less than 0.01 g/100 L at 100 mm Hg [1] but the effect on leukocytes, vWF multimers and platelets are unknown. Here, the CentriMag® was tested using bovine blood (n=15) under constant haemodynamic conditions in comparison to a static control in terms of total blood cell counts, haemolysis, leukocyte death, vWF multimers, microparticles, platelet activation and apoptosis. The significant difference of total blood damage compared to the static control, was that Although there was no significant difference in haemolysis, cell counts, platelet activation or apoptosis between pumped and static blood, the CentriMag® decreased the levels of healthy leukocytes (p&#60;0.006), induced some leukocyte microparticles (p&#60;10-5) and the level of high molecular weight of vWF multimers was significantly reduced in the CentriMag® compared to the static treatment (p&#60;10-5) after 6 hours in vitro testing. Despite the leukocyte damage, microparticle formation and cleavage of vWF multimers, these results show that the CentriMag® is a haemocompatible pump which could be used as a standard control for in blood damage assays to inform the design for new implantable blood pumps. Journal article Artificial Organs 39 2 93 101 0 0 2014 2014-01-01 10.1111/aor.12351 Swansea University Medical School CMED BMS Microbes and immunity None 2016-04-20T14:17:54Z 2014-09-02T21:38:13Z Swansea University Medical School Medicine Chris H.H. Chan 1 Ina Laura Pieper 2 Rebecca Hambly 3 Gemma Radley 4 Alyssa Jones 5 Yasmin Friedmann 6 Karl M. Hawkins 7 Stephen Westaby 8 Graham Foster 9 Catherine A. Thornton 10
title The CentriMag Centrifugal Blood Pump as a Benchmark for In Vitro Testing of Hemocompatibility in Implantable Ventricular Assist Devices
spellingShingle The CentriMag Centrifugal Blood Pump as a Benchmark for In Vitro Testing of Hemocompatibility in Implantable Ventricular Assist Devices
Thornton, Catherine
title_short The CentriMag Centrifugal Blood Pump as a Benchmark for In Vitro Testing of Hemocompatibility in Implantable Ventricular Assist Devices
title_full The CentriMag Centrifugal Blood Pump as a Benchmark for In Vitro Testing of Hemocompatibility in Implantable Ventricular Assist Devices
title_fullStr The CentriMag Centrifugal Blood Pump as a Benchmark for In Vitro Testing of Hemocompatibility in Implantable Ventricular Assist Devices
title_full_unstemmed The CentriMag Centrifugal Blood Pump as a Benchmark for In Vitro Testing of Hemocompatibility in Implantable Ventricular Assist Devices
title_sort The CentriMag Centrifugal Blood Pump as a Benchmark for In Vitro Testing of Hemocompatibility in Implantable Ventricular Assist Devices
author_id_str_mv c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv c71a7a4be7361094d046d312202bce0c_***_Thornton, Catherine
author Thornton, Catherine
author2 Chris H.H. Chan
Ina Laura Pieper
Rebecca Hambly
Gemma Radley
Alyssa Jones
Yasmin Friedmann
Karl M. Hawkins
Stephen Westaby
Graham Foster
Catherine A. Thornton
format Journal article
container_title Artificial Organs
container_volume 39
container_issue 2
container_start_page 93
publishDate 2014
institution Swansea University
doi_str_mv 10.1111/aor.12351
college_str Swansea University Medical School
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hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
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hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 0
active_str 1
researchgroup_str Microbes and immunity
description Implantable ventricular assist devices (VADs) have proven efficient in advanced heart failure patients as a bridge-to-transplant or destination therapy. However, VAD usage often leads to infection, bleeding and thrombosis, side effects attributable to the damage to blood cells and plasma proteins. We have shown previously that m Measuring haemolysis alone does not provide sufficient information to understand total blood damage, and research exploring the impact of currently available pumps on a wider range of blood cell types and plasma proteins such as von Willebrand factor (vWF) within blood is required to further our understanding of safer pump design. The extracorporeal CentriMag® has a haemolysis profile within published standards for haemolysis of NIH levels of less than 0.01 g/100 L at 100 mm Hg [1] but the effect on leukocytes, vWF multimers and platelets are unknown. Here, the CentriMag® was tested using bovine blood (n=15) under constant haemodynamic conditions in comparison to a static control in terms of total blood cell counts, haemolysis, leukocyte death, vWF multimers, microparticles, platelet activation and apoptosis. The significant difference of total blood damage compared to the static control, was that Although there was no significant difference in haemolysis, cell counts, platelet activation or apoptosis between pumped and static blood, the CentriMag® decreased the levels of healthy leukocytes (p&#60;0.006), induced some leukocyte microparticles (p&#60;10-5) and the level of high molecular weight of vWF multimers was significantly reduced in the CentriMag® compared to the static treatment (p&#60;10-5) after 6 hours in vitro testing. Despite the leukocyte damage, microparticle formation and cleavage of vWF multimers, these results show that the CentriMag® is a haemocompatible pump which could be used as a standard control for in blood damage assays to inform the design for new implantable blood pumps.
published_date 2014-01-01T04:22:08Z
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