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The role of microRNA-155/LXR pathway in experimental and Idiopathic Pulmonary Fibrosis / Mariola Kurowska-Stolarska; Manhl K. Hasoo; David J. Welsh; Lynn Stewart; Donna McIntyre; Brian E. Morton; Steven Johnstone; Ashley M. Miller; Darren L. Asquith; Neal L. Millar; Ann B. Millar; Carol A. Feghali-Bostwick; Nikhil Hirani; Peter J. Crick; Yuqin Wang; William J. Griffiths; Iain B. McInnes; Charles McSharry
Journal of Allergy and Clinical Immunology
Swansea University Author: Griffiths, William
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AbstractBackground: Idiopathic Pulmonary Fibrosis (IPF) is progressive and rapidly fatal. Improved understanding of pathogenesis is required to prosper novel therapeutics. Epigenetic changes contribute to IPF therefore microRNAs may reveal novel pathogenic pathways.Objectives: To determine the regul...
|Published in:||Journal of Allergy and Clinical Immunology|
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AbstractBackground: Idiopathic Pulmonary Fibrosis (IPF) is progressive and rapidly fatal. Improved understanding of pathogenesis is required to prosper novel therapeutics. Epigenetic changes contribute to IPF therefore microRNAs may reveal novel pathogenic pathways.Objectives: To determine the regulatory role of microRNA(miR)-155 in the pro-fibrotic function of murine lung macrophages and fibroblasts, IPF lung fibroblasts and its contribution to experimental pulmonary fibrosis.Methods: Bleomycin-induced lung fibrosis in wild-type and miR-155-/- mice was analyzed by histology, collagen and pro-fibrotic gene expression. Mechanisms were identified by in silico and molecular approaches; validated in mouse lung fibroblasts and macrophages, and in IPF lung fibroblasts, using loss-and-gain of function assays, and in vivo using specific inhibitors.Results: miR-155-/- mice developed exacerbated lung fibrosis, increased collagen deposition, collagen 1 and 3 mRNA expression, TGFβ production, and activation of alternatively-activated macrophages, contributed by deregulation of the microRNA-155 target gene the liver X receptor (LXR)α in lung fibroblasts and macrophages. Inhibition of LXRα in experimental lung fibrosis and in IPF lung fibroblasts reduced the exacerbated fibrotic response. Similarly, enforced expression of miR-155 reduced the pro-fibrotic phenotype of IPF and miR-155-/- fibroblasts.Conclusion: We describe herein a molecular pathway comprising miR-155 and its epigenetic LXRα target that when deregulated enables pathogenic pulmonary fibrosis. Manipulation of the miR-155/LXR pathway may have therapeutic potential for IPF.
The Swansea University team provided expert knowledge of LXR activity and generated the data presented in Supplemental Table E6
microRNA-155, lung fibrosis, Liver X Receptor, fibroblasts, alternatively activated macrophages
Swansea University Medical School