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The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells / Riccardo Levato; William R. Webb; Iris A. Otto; Anneloes Mensinga; Yadan Zhang; Mattie van Rijen; René van Weeren; Ilyas M. Khan; Jos Malda

Acta Biomaterialia, Volume: 61, Pages: 41 - 53

Swansea University Author: Khan, Ilyas

Abstract

Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of enc...

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Published in: Acta Biomaterialia
ISSN: 17427061
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa34908
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To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration. However, little is known about the suitability of ACPCs for tissue engineering, especially in combination with biomaterials. This study aimed to investigate the potential of ACPCs in hydrogels for cartilage regeneration and biofabrication, and to evaluate their ability for zone-specific matrix production. Gelatin methacryloyl (gelMA)-based hydrogels were used to culture ACPCs, bone marrow mesenchymal stromal cells (MSCs) and chondrocytes, and as bioinks for printing. Our data shows ACPCs outperformed chondrocytes in terms of neo-cartilage production and unlike MSCs, ACPCs had the lowest gene expression levels of hypertrophy marker collagen type X, and the highest expression of PRG4, a key factor in joint lubrication. Co-cultures of the cell types in multi-compartment hydrogels allowed generating constructs with a layered distribution of collagens and glycosaminoglycans. By combining ACPC- and MSC-laden bioinks, a bioprinted model of articular cartilage was generated, consisting of defined superficial and deep regions, each with distinct cellular and extracellular matrix composition. 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spelling 2018-02-08T12:51:42Z v2 34908 2017-08-14 The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells Ilyas Khan Ilyas Khan true 0000-0002-3886-1987 false 2536d955ff70e7b77063a8efe9103161 3df2d4e694473d044744d12c702fba24 O8AaXfb/kO0Gte3TBYMZR9yvqZQRJmUl2lxhnzSZE7o= 2017-08-14 PMSC Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration. However, little is known about the suitability of ACPCs for tissue engineering, especially in combination with biomaterials. This study aimed to investigate the potential of ACPCs in hydrogels for cartilage regeneration and biofabrication, and to evaluate their ability for zone-specific matrix production. Gelatin methacryloyl (gelMA)-based hydrogels were used to culture ACPCs, bone marrow mesenchymal stromal cells (MSCs) and chondrocytes, and as bioinks for printing. Our data shows ACPCs outperformed chondrocytes in terms of neo-cartilage production and unlike MSCs, ACPCs had the lowest gene expression levels of hypertrophy marker collagen type X, and the highest expression of PRG4, a key factor in joint lubrication. Co-cultures of the cell types in multi-compartment hydrogels allowed generating constructs with a layered distribution of collagens and glycosaminoglycans. By combining ACPC- and MSC-laden bioinks, a bioprinted model of articular cartilage was generated, consisting of defined superficial and deep regions, each with distinct cellular and extracellular matrix composition. Taken together, these results provide important information for the use of ACPC-laden hydrogels in regenerative medicine, and pave the way to the biofabrication of 3D constructs with multiple cell types for cartilage regeneration or in vitro tissue models. Journal article Acta Biomaterialia 61 41 53 17427061 1 10 2017 2017-10-01 10.1016/j.actbio.2017.08.005 Swansea University Medical School Medicine CMED PMSC Swansea University Devices United Kingdom Regenerative Medicine Platform (MRC) None 2018-02-08T12:51:42Z 2017-08-14T14:17:51Z Swansea University Medical School Medicine Riccardo Levato 1 William R. Webb 2 Iris A. Otto 3 Anneloes Mensinga 4 Yadan Zhang 5 Mattie van Rijen 6 René van Weeren 7 Ilyas M. Khan 8 Jos Malda 9 0034908-07092017160707.pdf WWebbv2.pdf 2017-09-07T16:07:07Z Output 9803633 application/pdf AM true Updated Copyright 07/09/2017 2018-08-04T00:00:00 12 month embargo. true eng
title The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
spellingShingle The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
Khan, Ilyas
title_short The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
title_full The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
title_fullStr The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
title_full_unstemmed The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
title_sort The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
author_id_str_mv 2536d955ff70e7b77063a8efe9103161
author_id_fullname_str_mv 2536d955ff70e7b77063a8efe9103161_***_Khan, Ilyas
author Khan, Ilyas
author2 Riccardo Levato
William R. Webb
Iris A. Otto
Anneloes Mensinga
Yadan Zhang
Mattie van Rijen
René van Weeren
Ilyas M. Khan
Jos Malda
format Journal article
container_title Acta Biomaterialia
container_volume 61
container_start_page 41
publishDate 2017
institution Swansea University
issn 17427061
doi_str_mv 10.1016/j.actbio.2017.08.005
college_str Swansea University Medical School
hierarchytype
hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 1
active_str 1
researchgroup_str Devices
description Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration. However, little is known about the suitability of ACPCs for tissue engineering, especially in combination with biomaterials. This study aimed to investigate the potential of ACPCs in hydrogels for cartilage regeneration and biofabrication, and to evaluate their ability for zone-specific matrix production. Gelatin methacryloyl (gelMA)-based hydrogels were used to culture ACPCs, bone marrow mesenchymal stromal cells (MSCs) and chondrocytes, and as bioinks for printing. Our data shows ACPCs outperformed chondrocytes in terms of neo-cartilage production and unlike MSCs, ACPCs had the lowest gene expression levels of hypertrophy marker collagen type X, and the highest expression of PRG4, a key factor in joint lubrication. Co-cultures of the cell types in multi-compartment hydrogels allowed generating constructs with a layered distribution of collagens and glycosaminoglycans. By combining ACPC- and MSC-laden bioinks, a bioprinted model of articular cartilage was generated, consisting of defined superficial and deep regions, each with distinct cellular and extracellular matrix composition. Taken together, these results provide important information for the use of ACPC-laden hydrogels in regenerative medicine, and pave the way to the biofabrication of 3D constructs with multiple cell types for cartilage regeneration or in vitro tissue models.
published_date 2017-10-01T05:43:57Z
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