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Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor

Jakub Trzebinski , Anna Radomska-Botelho Moniz, Sanjiv Sharma Orcid Logo, Krishna Burugapalli, Francis Moussy, Anthony E. G. Cass

Electroanalysis, Volume: 23, Issue: 12, Pages: 2789 - 2795

Swansea University Author: Sanjiv Sharma Orcid Logo

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DOI (Published version): 10.1002/elan.201100286

Abstract

A permselective membrane is a critical component that defines the linear detection limits, the sensitivity, and thus the ultimate efficacy of an enzymatic biosensor. Although membranes like epoxy-polyurethane (epoxy-PU) and Nafion are widely used and provide the desired glucose detection limits of 2...

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Published in: Electroanalysis
ISSN: 1040-0397
Published: Wiley-Blackwell 2011
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URI: https://cronfa.swan.ac.uk/Record/cronfa36259
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spelling 2017-10-26T09:21:00.9640565 v2 36259 2017-10-25 Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor b6b7506358522f607b171ec9c94757b7 0000-0003-3828-737X Sanjiv Sharma Sanjiv Sharma true false 2017-10-25 MEDE A permselective membrane is a critical component that defines the linear detection limits, the sensitivity, and thus the ultimate efficacy of an enzymatic biosensor. Although membranes like epoxy-polyurethane (epoxy-PU) and Nafion are widely used and provide the desired glucose detection limits of 2 to 30 mM, both the within batch and batch-to-batch variability of sensors that use these materials is a concern. The hypothesis for this study was that a crosslinked hydrogel would have a sufficiently uniform porosity and hydrophilicity to address the variability in sensor sensitivity. The hydrogel was prepared by crosslinking di-hydroxyethyl methacrylate, hydroxyethyl methacrylate and N-vinyl pyrrolidone with 2.5 mol% ethylene glycol dimethacrylate using water soluble initiators – ammonium persulfate and sodium metabisulfite under a nitrogen atmosphere. The hydrogel was applied to the sensor by dip coating during polymerisation. Electrochemical measurements revealed that the response characteristics of sensors coated with this membrane are highly consistent. Scanning electrochemical microscopy (SECM) was used to spatially resolve glucose diffusion through the membrane by measuring the consequent H2O2 release and compared with an epoxy-PU membrane. Hydrogen peroxide measurements using SECM revealed that the epoxy-PU membranes had uneven lateral diffusion profiles compared to the uniform profile of the hydrogel membranes. The uneven diffusion profiles of epoxy-PU membranes are attributed to a fabrication method that results in uneven membrane properties, while the uniform diffusion profiles of the hydrogel membranes are primarily dictated by their uniform pore size. Journal Article Electroanalysis 23 12 2789 2795 Wiley-Blackwell 1040-0397 31 12 2011 2011-12-31 10.1002/elan.201100286 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2017-10-26T09:21:00.9640565 2017-10-25T14:06:27.1406018 College of Engineering Engineering Jakub Trzebinski  1 Anna Radomska-Botelho Moniz 2 Sanjiv Sharma 0000-0003-3828-737X 3 Krishna Burugapalli 4 Francis Moussy 5 Anthony E. G. Cass 6
title Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor
spellingShingle Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor
Sanjiv Sharma
title_short Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor
title_full Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor
title_fullStr Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor
title_full_unstemmed Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor
title_sort Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor
author_id_str_mv b6b7506358522f607b171ec9c94757b7
author_id_fullname_str_mv b6b7506358522f607b171ec9c94757b7_***_Sanjiv Sharma
author Sanjiv Sharma
author2 Jakub Trzebinski 
Anna Radomska-Botelho Moniz
Sanjiv Sharma
Krishna Burugapalli
Francis Moussy
Anthony E. G. Cass
format Journal article
container_title Electroanalysis
container_volume 23
container_issue 12
container_start_page 2789
publishDate 2011
institution Swansea University
issn 1040-0397
doi_str_mv 10.1002/elan.201100286
publisher Wiley-Blackwell
college_str College of Engineering
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hierarchy_top_title College of Engineering
hierarchy_parent_id collegeofengineering
hierarchy_parent_title College of Engineering
department_str Engineering{{{_:::_}}}College of Engineering{{{_:::_}}}Engineering
document_store_str 0
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description A permselective membrane is a critical component that defines the linear detection limits, the sensitivity, and thus the ultimate efficacy of an enzymatic biosensor. Although membranes like epoxy-polyurethane (epoxy-PU) and Nafion are widely used and provide the desired glucose detection limits of 2 to 30 mM, both the within batch and batch-to-batch variability of sensors that use these materials is a concern. The hypothesis for this study was that a crosslinked hydrogel would have a sufficiently uniform porosity and hydrophilicity to address the variability in sensor sensitivity. The hydrogel was prepared by crosslinking di-hydroxyethyl methacrylate, hydroxyethyl methacrylate and N-vinyl pyrrolidone with 2.5 mol% ethylene glycol dimethacrylate using water soluble initiators – ammonium persulfate and sodium metabisulfite under a nitrogen atmosphere. The hydrogel was applied to the sensor by dip coating during polymerisation. Electrochemical measurements revealed that the response characteristics of sensors coated with this membrane are highly consistent. Scanning electrochemical microscopy (SECM) was used to spatially resolve glucose diffusion through the membrane by measuring the consequent H2O2 release and compared with an epoxy-PU membrane. Hydrogen peroxide measurements using SECM revealed that the epoxy-PU membranes had uneven lateral diffusion profiles compared to the uniform profile of the hydrogel membranes. The uneven diffusion profiles of epoxy-PU membranes are attributed to a fabrication method that results in uneven membrane properties, while the uniform diffusion profiles of the hydrogel membranes are primarily dictated by their uniform pore size.
published_date 2011-12-31T03:49:18Z
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