Journal article 944 views
Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor
Jakub Trzebinski ,
Anna Radomska-Botelho Moniz,
Sanjiv Sharma ,
Krishna Burugapalli,
Francis Moussy,
Anthony E. G. Cass
Electroanalysis, Volume: 23, Issue: 12, Pages: 2789 - 2795
Swansea University Author: Sanjiv Sharma
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DOI (Published version): 10.1002/elan.201100286
Abstract
A permselective membrane is a critical component that defines the linear detection limits, the sensitivity, and thus the ultimate efficacy of an enzymatic biosensor. Although membranes like epoxy-polyurethane (epoxy-PU) and Nafion are widely used and provide the desired glucose detection limits of 2...
Published in: | Electroanalysis |
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ISSN: | 1040-0397 |
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Wiley-Blackwell
2011
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URI: | https://cronfa.swan.ac.uk/Record/cronfa36259 |
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<?xml version="1.0"?><rfc1807><datestamp>2017-10-26T09:21:00.9640565</datestamp><bib-version>v2</bib-version><id>36259</id><entry>2017-10-25</entry><title>Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor</title><swanseaauthors><author><sid>b6b7506358522f607b171ec9c94757b7</sid><ORCID>0000-0003-3828-737X</ORCID><firstname>Sanjiv</firstname><surname>Sharma</surname><name>Sanjiv Sharma</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2017-10-25</date><deptcode>MEDE</deptcode><abstract>A permselective membrane is a critical component that defines the linear detection limits, the sensitivity, and thus the ultimate efficacy of an enzymatic biosensor. Although membranes like epoxy-polyurethane (epoxy-PU) and Nafion are widely used and provide the desired glucose detection limits of 2 to 30 mM, both the within batch and batch-to-batch variability of sensors that use these materials is a concern. The hypothesis for this study was that a crosslinked hydrogel would have a sufficiently uniform porosity and hydrophilicity to address the variability in sensor sensitivity. The hydrogel was prepared by crosslinking di-hydroxyethyl methacrylate, hydroxyethyl methacrylate and N-vinyl pyrrolidone with 2.5 mol% ethylene glycol dimethacrylate using water soluble initiators – ammonium persulfate and sodium metabisulfite under a nitrogen atmosphere. The hydrogel was applied to the sensor by dip coating during polymerisation. Electrochemical measurements revealed that the response characteristics of sensors coated with this membrane are highly consistent. Scanning electrochemical microscopy (SECM) was used to spatially resolve glucose diffusion through the membrane by measuring the consequent H2O2 release and compared with an epoxy-PU membrane. Hydrogen peroxide measurements using SECM revealed that the epoxy-PU membranes had uneven lateral diffusion profiles compared to the uniform profile of the hydrogel membranes. The uneven diffusion profiles of epoxy-PU membranes are attributed to a fabrication method that results in uneven membrane properties, while the uniform diffusion profiles of the hydrogel membranes are primarily dictated by their uniform pore size.</abstract><type>Journal Article</type><journal>Electroanalysis</journal><volume>23</volume><journalNumber>12</journalNumber><paginationStart>2789</paginationStart><paginationEnd>2795</paginationEnd><publisher>Wiley-Blackwell</publisher><issnPrint>1040-0397</issnPrint><keywords/><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2011</publishedYear><publishedDate>2011-12-31</publishedDate><doi>10.1002/elan.201100286</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MEDE</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2017-10-26T09:21:00.9640565</lastEdited><Created>2017-10-25T14:06:27.1406018</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Biomedical Engineering</level></path><authors><author><firstname>Jakub</firstname><surname>Trzebinski </surname><order>1</order></author><author><firstname>Anna Radomska-Botelho</firstname><surname>Moniz</surname><order>2</order></author><author><firstname>Sanjiv</firstname><surname>Sharma</surname><orcid>0000-0003-3828-737X</orcid><order>3</order></author><author><firstname>Krishna</firstname><surname>Burugapalli</surname><order>4</order></author><author><firstname>Francis</firstname><surname>Moussy</surname><order>5</order></author><author><firstname>Anthony E. G.</firstname><surname>Cass</surname><order>6</order></author></authors><documents/><OutputDurs/></rfc1807> |
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2017-10-26T09:21:00.9640565 v2 36259 2017-10-25 Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor b6b7506358522f607b171ec9c94757b7 0000-0003-3828-737X Sanjiv Sharma Sanjiv Sharma true false 2017-10-25 MEDE A permselective membrane is a critical component that defines the linear detection limits, the sensitivity, and thus the ultimate efficacy of an enzymatic biosensor. Although membranes like epoxy-polyurethane (epoxy-PU) and Nafion are widely used and provide the desired glucose detection limits of 2 to 30 mM, both the within batch and batch-to-batch variability of sensors that use these materials is a concern. The hypothesis for this study was that a crosslinked hydrogel would have a sufficiently uniform porosity and hydrophilicity to address the variability in sensor sensitivity. The hydrogel was prepared by crosslinking di-hydroxyethyl methacrylate, hydroxyethyl methacrylate and N-vinyl pyrrolidone with 2.5 mol% ethylene glycol dimethacrylate using water soluble initiators – ammonium persulfate and sodium metabisulfite under a nitrogen atmosphere. The hydrogel was applied to the sensor by dip coating during polymerisation. Electrochemical measurements revealed that the response characteristics of sensors coated with this membrane are highly consistent. Scanning electrochemical microscopy (SECM) was used to spatially resolve glucose diffusion through the membrane by measuring the consequent H2O2 release and compared with an epoxy-PU membrane. Hydrogen peroxide measurements using SECM revealed that the epoxy-PU membranes had uneven lateral diffusion profiles compared to the uniform profile of the hydrogel membranes. The uneven diffusion profiles of epoxy-PU membranes are attributed to a fabrication method that results in uneven membrane properties, while the uniform diffusion profiles of the hydrogel membranes are primarily dictated by their uniform pore size. Journal Article Electroanalysis 23 12 2789 2795 Wiley-Blackwell 1040-0397 31 12 2011 2011-12-31 10.1002/elan.201100286 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2017-10-26T09:21:00.9640565 2017-10-25T14:06:27.1406018 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Jakub Trzebinski 1 Anna Radomska-Botelho Moniz 2 Sanjiv Sharma 0000-0003-3828-737X 3 Krishna Burugapalli 4 Francis Moussy 5 Anthony E. G. Cass 6 |
title |
Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor |
spellingShingle |
Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor Sanjiv Sharma |
title_short |
Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor |
title_full |
Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor |
title_fullStr |
Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor |
title_full_unstemmed |
Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor |
title_sort |
Hydrogel Membrane Improves Batch-to-Batch Reproducibility of an Enzymatic Glucose Biosensor |
author_id_str_mv |
b6b7506358522f607b171ec9c94757b7 |
author_id_fullname_str_mv |
b6b7506358522f607b171ec9c94757b7_***_Sanjiv Sharma |
author |
Sanjiv Sharma |
author2 |
Jakub Trzebinski Anna Radomska-Botelho Moniz Sanjiv Sharma Krishna Burugapalli Francis Moussy Anthony E. G. Cass |
format |
Journal article |
container_title |
Electroanalysis |
container_volume |
23 |
container_issue |
12 |
container_start_page |
2789 |
publishDate |
2011 |
institution |
Swansea University |
issn |
1040-0397 |
doi_str_mv |
10.1002/elan.201100286 |
publisher |
Wiley-Blackwell |
college_str |
Faculty of Science and Engineering |
hierarchytype |
|
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facultyofscienceandengineering |
hierarchy_top_title |
Faculty of Science and Engineering |
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facultyofscienceandengineering |
hierarchy_parent_title |
Faculty of Science and Engineering |
department_str |
School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering |
document_store_str |
0 |
active_str |
0 |
description |
A permselective membrane is a critical component that defines the linear detection limits, the sensitivity, and thus the ultimate efficacy of an enzymatic biosensor. Although membranes like epoxy-polyurethane (epoxy-PU) and Nafion are widely used and provide the desired glucose detection limits of 2 to 30 mM, both the within batch and batch-to-batch variability of sensors that use these materials is a concern. The hypothesis for this study was that a crosslinked hydrogel would have a sufficiently uniform porosity and hydrophilicity to address the variability in sensor sensitivity. The hydrogel was prepared by crosslinking di-hydroxyethyl methacrylate, hydroxyethyl methacrylate and N-vinyl pyrrolidone with 2.5 mol% ethylene glycol dimethacrylate using water soluble initiators – ammonium persulfate and sodium metabisulfite under a nitrogen atmosphere. The hydrogel was applied to the sensor by dip coating during polymerisation. Electrochemical measurements revealed that the response characteristics of sensors coated with this membrane are highly consistent. Scanning electrochemical microscopy (SECM) was used to spatially resolve glucose diffusion through the membrane by measuring the consequent H2O2 release and compared with an epoxy-PU membrane. Hydrogen peroxide measurements using SECM revealed that the epoxy-PU membranes had uneven lateral diffusion profiles compared to the uniform profile of the hydrogel membranes. The uneven diffusion profiles of epoxy-PU membranes are attributed to a fabrication method that results in uneven membrane properties, while the uniform diffusion profiles of the hydrogel membranes are primarily dictated by their uniform pore size. |
published_date |
2011-12-31T03:45:17Z |
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1763752140739706880 |
score |
11.028886 |