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Prevalence of sickle cell gene in Yemen. / Hafiz Abdul Hamid Al-Nood

Swansea University Author: Hafiz Abdul Hamid, Al-Nood

Abstract

To determine the prevalence of the sickle cell gene (HbS) in Yemen and amongst people from different regions of the country living in the capital, Sana'a City, cord blood samples from 1500 consented mothers were collected from hospitals in Sana'a City between July and December 2001. The na...

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Published: 2004
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa42767
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Abstract: To determine the prevalence of the sickle cell gene (HbS) in Yemen and amongst people from different regions of the country living in the capital, Sana'a City, cord blood samples from 1500 consented mothers were collected from hospitals in Sana'a City between July and December 2001. The names and original homes of the parents were recorded. Cationic HPLC analysis was used for screening while isoelectric focusing (IEF) and DNA- PCR were used to confirm haemoglobin S (HbS). Thirty-three samples were found to show Hb FAS giving an overall likely Hb S gene frequency of 0.011. The Hb S gene frequency varied with the part of the country from which the parents came. Amongst people from Taiz and Haja in the west the gene frequency was more than 0.04 but less than 0.004 amongst people from Ibb, adjacent to the governorate of Taiz. Of 66 chromosomes from babies carrying HbS, only 1.5% additionally carried the presence of-158 (C→T) G-gamma globin gene Xmm I site compared with 16.1% of 168 chromosomes from babies without Hb S from the same regions of the sickle cell trait samples identified in this study indicated that the beta S haplotype in not that associated with a milder course found in east Saudi Arabia. In addition to the absence of both Hind III/Ggamma and Hind III/Agamma beta globin polymorphic sites in 26 sickle cell trait samples suggesting the predominant of the African sickle cell haplotype (Benin) in Yemen. The results of this study thus show a higher Hb S gene frequency in the western coastal part of Yemen than in the central mountainous and eastern desert areas. The incidence of affected homozygous births may therefore reach 20/10,000 in the western coastal part of Yemen. A survey to evaluate health care of sickle cell patients was performed using 86 patients attending hospitals in Sanaa City, Yemen. The results showed that the clinical services provided to the sickle cell patients in Yemen were generally very poor. Limited health resources can best be invested in developing a program of (Education, screening and health care initially prioritising those communities residing in the western areas of Yemen with the highest Hb S gene frequency.
Keywords: Genetics.
College: Swansea University Medical School