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Investigating Metabolism and Competition In Staphylococcus epidermidis Biofilms / EBONY BANSAIR

Swansea University Author: EBONY BANSAIR

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Abstract

Staphylococcus epidermidis is a commensal bacterium of the skin implicated as a significant cause of medical device infections, causing around 20% of orthopaedic device related infections and 13% of prosthetic valve endocarditis infections. A major virulence factor of this bacterium is its ability t...

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Published: Swansea 2022
Institution: Swansea University
Degree level: Master of Research
Degree name: MSc by Research
Supervisor: Harris Llinos G. ; Wilkinson, Thomas S.
URI: https://cronfa.swan.ac.uk/Record/cronfa60283
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first_indexed 2022-06-20T16:17:13Z
last_indexed 2022-06-21T03:19:11Z
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spelling 2022-06-20T17:27:00.5916225 v2 60283 2022-06-20 Investigating Metabolism and Competition In Staphylococcus epidermidis Biofilms 78ac29c5f9dfd73c8be8cf8b1b3b5086 EBONY BANSAIR EBONY BANSAIR true false 2022-06-20 Staphylococcus epidermidis is a commensal bacterium of the skin implicated as a significant cause of medical device infections, causing around 20% of orthopaedic device related infections and 13% of prosthetic valve endocarditis infections. A major virulence factor of this bacterium is its ability to adhere to surfaces and form biofilms. In a biofilm environment there are qualities that aid endurance of the bacteria inside the body, by antibiotic resistance and evasion of host defences. S. epidermidis in a biofilm environment display different properties to those in a planktonic environment, such as slowed growth cycles and altered metabolism thus enhancing its survival. An association has already been made between the tricarboxylic acid cycle and the regulatory mechanisms for the formation of polysaccharide intercellular adhesin. However, our knowledge on the effects of metabolism on the mechanisms used by protein mediated biofilms to cause persistent infections is limited. Therefore, the aim of this study is to investigate the influence of metabolic pathways on different S. epidermidis biofilm formation mechanisms and the relationship between metabolism and quorum sensing mechanisms of regulation in protein mediated biofilms. Metabolism was measured for three S. epidermidis strains that are known to form biofilms using different mechanisms using the fluorescent redox stains 5-cyano-2,3-ditolyl tetrazolium chloride and resazurin. Biofilm positive isolates showed more reduction than biofilm negative isolates. Additionally, the addition of spent media from biofilm positive isolates was able to modulate biofilm formation in both biofilm positive and negative isolates. Further development of this research could facilitate the identification of specific metabolic differences between protein and polysaccharide mediated biofilms, and between the effects of quorum sensing in protein mediated biofilms. E-Thesis Swansea 15 6 2022 2022-06-15 COLLEGE NANME COLLEGE CODE Swansea University Harris Llinos G. ; Wilkinson, Thomas S. Master of Research MSc by Research 2022-06-20T17:27:00.5916225 2022-06-20T17:13:36.3948559 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine EBONY BANSAIR 1 60283__24342__34d844238b35421e929a16e0c87c22c4.pdf Bansair_Ebony_R_G_MSc_Research_Thesis_Final_Redacted_Signature.pdf 2022-06-20T17:21:33.6578996 Output 3276244 application/pdf E-Thesis – open access true Copyright: The author, Ebony R. G. Bansair, 2022. true eng
title Investigating Metabolism and Competition In Staphylococcus epidermidis Biofilms
spellingShingle Investigating Metabolism and Competition In Staphylococcus epidermidis Biofilms
EBONY BANSAIR
title_short Investigating Metabolism and Competition In Staphylococcus epidermidis Biofilms
title_full Investigating Metabolism and Competition In Staphylococcus epidermidis Biofilms
title_fullStr Investigating Metabolism and Competition In Staphylococcus epidermidis Biofilms
title_full_unstemmed Investigating Metabolism and Competition In Staphylococcus epidermidis Biofilms
title_sort Investigating Metabolism and Competition In Staphylococcus epidermidis Biofilms
author_id_str_mv 78ac29c5f9dfd73c8be8cf8b1b3b5086
author_id_fullname_str_mv 78ac29c5f9dfd73c8be8cf8b1b3b5086_***_EBONY BANSAIR
author EBONY BANSAIR
author2 EBONY BANSAIR
format E-Thesis
publishDate 2022
institution Swansea University
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description Staphylococcus epidermidis is a commensal bacterium of the skin implicated as a significant cause of medical device infections, causing around 20% of orthopaedic device related infections and 13% of prosthetic valve endocarditis infections. A major virulence factor of this bacterium is its ability to adhere to surfaces and form biofilms. In a biofilm environment there are qualities that aid endurance of the bacteria inside the body, by antibiotic resistance and evasion of host defences. S. epidermidis in a biofilm environment display different properties to those in a planktonic environment, such as slowed growth cycles and altered metabolism thus enhancing its survival. An association has already been made between the tricarboxylic acid cycle and the regulatory mechanisms for the formation of polysaccharide intercellular adhesin. However, our knowledge on the effects of metabolism on the mechanisms used by protein mediated biofilms to cause persistent infections is limited. Therefore, the aim of this study is to investigate the influence of metabolic pathways on different S. epidermidis biofilm formation mechanisms and the relationship between metabolism and quorum sensing mechanisms of regulation in protein mediated biofilms. Metabolism was measured for three S. epidermidis strains that are known to form biofilms using different mechanisms using the fluorescent redox stains 5-cyano-2,3-ditolyl tetrazolium chloride and resazurin. Biofilm positive isolates showed more reduction than biofilm negative isolates. Additionally, the addition of spent media from biofilm positive isolates was able to modulate biofilm formation in both biofilm positive and negative isolates. Further development of this research could facilitate the identification of specific metabolic differences between protein and polysaccharide mediated biofilms, and between the effects of quorum sensing in protein mediated biofilms.
published_date 2022-06-15T04:18:16Z
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score 10.99342