No Cover Image

Journal article 575 views

Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B. / CM Martel; JE Parker; Warrilow AGS; N Rolley; SL Kelly; DE Kelly; Steven Kelly

Antimicrobial Agents and Chemotherapy, Volume: 54, Issue: 11, Pages: 4920 - 3

Swansea University Author: Steven, Kelly

Full text not available from this repository: check for access using links below.

DOI (Published version): 10.1128/AAC.00349-10

Abstract

Aspergillus fumigatus sterol 14 alpha-demethylase isoenzymes CYP51A and CYP51B were heterologously expressed in a Saccharomyces cerevisiae mutant (YUG37-erg11), wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. When cultured in the presence of doxycycline,...

Full description

Published in: Antimicrobial Agents and Chemotherapy
Published: American Society of Microbiology 2010
URI: https://cronfa.swan.ac.uk/Record/cronfa9903
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2013-07-23T12:02:23Z
last_indexed 2018-02-09T04:38:23Z
id cronfa9903
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2013-08-28T05:58:23.3494315</datestamp><bib-version>v2</bib-version><id>9903</id><entry>2012-03-20</entry><title>Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14&#x3B1;-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.</title><swanseaauthors><author><sid>b17cebaf09b4d737b9378a3581e3de93</sid><ORCID>0000-0001-7991-5040</ORCID><firstname>Steven</firstname><surname>Kelly</surname><name>Steven Kelly</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2012-03-20</date><deptcode>BMS</deptcode><abstract>Aspergillus fumigatus sterol 14 alpha-demethylase isoenzymes CYP51A and CYP51B were heterologously expressed in a Saccharomyces cerevisiae mutant (YUG37-erg11), wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. When cultured in the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts were able to synthesize ergosterol and grow; a control strain harboring reverse-oriented cyp51A could not. YUG37-pcyp51A and YUG37-pcyp51B constructs showed identical sensitivity to itraconazole, posaconazole, clotrimazole, and voriconazole. Conversely, YUG37-pcyp51A withstood 16-fold-higher concentrations of fluconazole than YUG37-pcyp51B (8 and 0.5 mu g ml(-1), respectively).</abstract><type>Journal Article</type><journal>Antimicrobial Agents and Chemotherapy</journal><volume>54</volume><journalNumber>11</journalNumber><paginationStart>4920</paginationStart><paginationEnd>3</paginationEnd><publisher>American Society of Microbiology</publisher><placeOfPublication/><issnPrint/><issnElectronic/><keywords>INVASIVE FUNGAL-INFECTIONS; AMINO-ACID SUBSTITUTION; CANDIDA-ALBICANS; ITRACONAZOLE RESISTANCE; FLUCONAZOLE RESISTANCE; REDUCED AFFINITY; AMPHOTERICIN-B; DEMETHYLASE; POSACONAZOLE; ANTIFUNGALS</keywords><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2010</publishedYear><publishedDate>2010-12-31</publishedDate><doi>10.1128/AAC.00349-10</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2013-08-28T05:58:23.3494315</lastEdited><Created>2012-03-20T10:06:59.3686858</Created><path><level id="1">Swansea University Medical School</level><level id="2">Medicine</level></path><authors><author><firstname>CM</firstname><surname>Martel</surname><order>1</order></author><author><firstname>JE</firstname><surname>Parker</surname><order>2</order></author><author><firstname>Warrilow</firstname><surname>AGS</surname><order>3</order></author><author><firstname>N</firstname><surname>Rolley</surname><order>4</order></author><author><firstname>SL</firstname><surname>Kelly</surname><order>5</order></author><author><firstname>DE</firstname><surname>Kelly</surname><order>6</order></author><author><firstname>Steven</firstname><surname>Kelly</surname><orcid>0000-0001-7991-5040</orcid><order>7</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2013-08-28T05:58:23.3494315 v2 9903 2012-03-20 Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B. b17cebaf09b4d737b9378a3581e3de93 0000-0001-7991-5040 Steven Kelly Steven Kelly true false 2012-03-20 BMS Aspergillus fumigatus sterol 14 alpha-demethylase isoenzymes CYP51A and CYP51B were heterologously expressed in a Saccharomyces cerevisiae mutant (YUG37-erg11), wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. When cultured in the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts were able to synthesize ergosterol and grow; a control strain harboring reverse-oriented cyp51A could not. YUG37-pcyp51A and YUG37-pcyp51B constructs showed identical sensitivity to itraconazole, posaconazole, clotrimazole, and voriconazole. Conversely, YUG37-pcyp51A withstood 16-fold-higher concentrations of fluconazole than YUG37-pcyp51B (8 and 0.5 mu g ml(-1), respectively). Journal Article Antimicrobial Agents and Chemotherapy 54 11 4920 3 American Society of Microbiology INVASIVE FUNGAL-INFECTIONS; AMINO-ACID SUBSTITUTION; CANDIDA-ALBICANS; ITRACONAZOLE RESISTANCE; FLUCONAZOLE RESISTANCE; REDUCED AFFINITY; AMPHOTERICIN-B; DEMETHYLASE; POSACONAZOLE; ANTIFUNGALS 31 12 2010 2010-12-31 10.1128/AAC.00349-10 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2013-08-28T05:58:23.3494315 2012-03-20T10:06:59.3686858 Swansea University Medical School Medicine CM Martel 1 JE Parker 2 Warrilow AGS 3 N Rolley 4 SL Kelly 5 DE Kelly 6 Steven Kelly 0000-0001-7991-5040 7
title Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.
spellingShingle Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.
Steven, Kelly
title_short Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.
title_full Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.
title_fullStr Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.
title_full_unstemmed Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.
title_sort Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.
author_id_str_mv b17cebaf09b4d737b9378a3581e3de93
author_id_fullname_str_mv b17cebaf09b4d737b9378a3581e3de93_***_Steven, Kelly
author Steven, Kelly
author2 CM Martel
JE Parker
Warrilow AGS
N Rolley
SL Kelly
DE Kelly
Steven Kelly
format Journal article
container_title Antimicrobial Agents and Chemotherapy
container_volume 54
container_issue 11
container_start_page 4920
publishDate 2010
institution Swansea University
doi_str_mv 10.1128/AAC.00349-10
publisher American Society of Microbiology
college_str Swansea University Medical School
hierarchytype
hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 0
active_str 0
description Aspergillus fumigatus sterol 14 alpha-demethylase isoenzymes CYP51A and CYP51B were heterologously expressed in a Saccharomyces cerevisiae mutant (YUG37-erg11), wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. When cultured in the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts were able to synthesize ergosterol and grow; a control strain harboring reverse-oriented cyp51A could not. YUG37-pcyp51A and YUG37-pcyp51B constructs showed identical sensitivity to itraconazole, posaconazole, clotrimazole, and voriconazole. Conversely, YUG37-pcyp51A withstood 16-fold-higher concentrations of fluconazole than YUG37-pcyp51B (8 and 0.5 mu g ml(-1), respectively).
published_date 2010-12-31T03:21:52Z
_version_ 1697796075847417856
score 10.789898