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Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe

B Palabiyik, S Karaer, N Arda, SE Toker, G Temizkan, Steven Kelly, A Topal Sarikaya

Biologia, Volume: 63, Issue: 1, Pages: 450 - 454

Swansea University Author: Steven Kelly

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DOI (Published version): 10.2478/s11756-008-0069-0

Abstract

Heterologous expression systems can be utilized to great advantage in the study of cytochrome P450 enzymes.P450 3A4 is one of the major forms of cytochrome P450 found in liver. It is also involved in the metabolism of numerouswidely used drugs and xenobiotics. In the present study human liver cytoch...

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Published in: Biologia
Published: Springer 2008
URI: https://cronfa.swan.ac.uk/Record/cronfa10676
first_indexed 2013-07-23T12:04:02Z
last_indexed 2021-10-30T02:21:56Z
id cronfa10676
recordtype SURis
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spelling 2021-10-29T09:43:45.2511638 v2 10676 2012-04-19 Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe b17cebaf09b4d737b9378a3581e3de93 Steven Kelly Steven Kelly true false 2012-04-19 Heterologous expression systems can be utilized to great advantage in the study of cytochrome P450 enzymes.P450 3A4 is one of the major forms of cytochrome P450 found in liver. It is also involved in the metabolism of numerouswidely used drugs and xenobiotics. In the present study human liver cytochrome P450 3A4 gene was transferred intothe fission yeast Schizosaccharomyces pombe via two different S. pombe expression vectors carrying thiamine repressiblepromoter – nmt1 (pREP42) and constitutive promoter – adh1 (pART1). Heterologously expressed cytochrome P450 3A4 wasdetected in the cells grown in minimal (EMM) or rich medium (YEL) containing 0.5% (w/v) glucose. A typical cytochromeP450 peak for 3A4 was observed at 448 nm in microsomal fraction. The presence of heterologous expression of 3A4 formwas also determined by SDS-PAGE and it molecular mass was identified as 52 kDa. The enzyme activity was confirmed byHPLC analysis, using testosterone as substrate. Journal Article Biologia 63 1 450 454 Springer 31 12 2008 2008-12-31 10.2478/s11756-008-0069-0 COLLEGE NANME COLLEGE CODE Swansea University 2021-10-29T09:43:45.2511638 2012-04-19T11:48:23.4828538 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine B Palabiyik 1 S Karaer 2 N Arda 3 SE Toker 4 G Temizkan 5 Steven Kelly 6 A Topal Sarikaya 7
title Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe
spellingShingle Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe
Steven Kelly
title_short Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe
title_full Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe
title_fullStr Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe
title_full_unstemmed Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe
title_sort Expression of human cytochrome p450 3A4 gene in Schizosaccharomyces pombe
author_id_str_mv b17cebaf09b4d737b9378a3581e3de93
author_id_fullname_str_mv b17cebaf09b4d737b9378a3581e3de93_***_Steven Kelly
author Steven Kelly
author2 B Palabiyik
S Karaer
N Arda
SE Toker
G Temizkan
Steven Kelly
A Topal Sarikaya
format Journal article
container_title Biologia
container_volume 63
container_issue 1
container_start_page 450
publishDate 2008
institution Swansea University
doi_str_mv 10.2478/s11756-008-0069-0
publisher Springer
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description Heterologous expression systems can be utilized to great advantage in the study of cytochrome P450 enzymes.P450 3A4 is one of the major forms of cytochrome P450 found in liver. It is also involved in the metabolism of numerouswidely used drugs and xenobiotics. In the present study human liver cytochrome P450 3A4 gene was transferred intothe fission yeast Schizosaccharomyces pombe via two different S. pombe expression vectors carrying thiamine repressiblepromoter – nmt1 (pREP42) and constitutive promoter – adh1 (pART1). Heterologously expressed cytochrome P450 3A4 wasdetected in the cells grown in minimal (EMM) or rich medium (YEL) containing 0.5% (w/v) glucose. A typical cytochromeP450 peak for 3A4 was observed at 448 nm in microsomal fraction. The presence of heterologous expression of 3A4 formwas also determined by SDS-PAGE and it molecular mass was identified as 52 kDa. The enzyme activity was confirmed byHPLC analysis, using testosterone as substrate.
published_date 2008-12-31T06:17:07Z
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score 11.059829

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