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Analysis of neurosterols by GC–MS and LC–MS/MS / William Griffiths, Yuqin Wang

Journal of Chromatography B, Volume: 877, Issue: 26, Pages: 2778 - 2805

Swansea University Author: William Griffiths

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Abstract

The term neurosteroid was coined by Baulieu and colleagues in Paris towards the end of the last century to describe steroids which are synthesised in the central or peripheral nervous system [E.E. Baulieu, Psychoneuroendocrinology 23 (1998) 963–87]. This definition was restricted to side-chain “shor...

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Published in: Journal of Chromatography B
ISSN: 1570-0232
Published: 2009
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URI: https://cronfa.swan.ac.uk/Record/cronfa10947
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spelling 2011-10-01T00:00:00.0000000 v2 10947 2012-06-05 Analysis of neurosterols by GC–MS and LC–MS/MS 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2012-06-05 BMS The term neurosteroid was coined by Baulieu and colleagues in Paris towards the end of the last century to describe steroids which are synthesised in the central or peripheral nervous system [E.E. Baulieu, Psychoneuroendocrinology 23 (1998) 963–87]. This definition was restricted to side-chain “shortened” steroids with 21 carbon atoms or less, and excluded sterols and their carboxylic acids with an intact side-chain. By analogy, we now use the term neurosterol to describe 27 carbon sterols synthesised in the nervous system. In this review we discuss the biological importance of neurosterols, and how they are extracted, isolated, and analysed by GC–MS and LC–MS/MS, from brain and relevant body fluids. We present applications of methodology employed for analysis of specific sterols and comment on the relative merits of the methods employed. Finally, the importance of future in-depth “sterolomic” investigations of brain is highlighted. Other Journal of Chromatography B 877 26 2778 2805 1570-0232 31 12 2009 2009-12-31 10.1016/j.jchromb.2009.05.017 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2011-10-01T00:00:00.0000000 2012-06-05T16:32:50.5349048 Swansea University Medical School Medicine William Griffiths 0000-0002-4129-6616 1 Yuqin Wang 2
title Analysis of neurosterols by GC–MS and LC–MS/MS
spellingShingle Analysis of neurosterols by GC–MS and LC–MS/MS
William, Griffiths
title_short Analysis of neurosterols by GC–MS and LC–MS/MS
title_full Analysis of neurosterols by GC–MS and LC–MS/MS
title_fullStr Analysis of neurosterols by GC–MS and LC–MS/MS
title_full_unstemmed Analysis of neurosterols by GC–MS and LC–MS/MS
title_sort Analysis of neurosterols by GC–MS and LC–MS/MS
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William, Griffiths
author William, Griffiths
author2 William Griffiths
Yuqin Wang
format Other
container_title Journal of Chromatography B
container_volume 877
container_issue 26
container_start_page 2778
publishDate 2009
institution Swansea University
issn 1570-0232
doi_str_mv 10.1016/j.jchromb.2009.05.017
college_str Swansea University Medical School
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hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 0
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description The term neurosteroid was coined by Baulieu and colleagues in Paris towards the end of the last century to describe steroids which are synthesised in the central or peripheral nervous system [E.E. Baulieu, Psychoneuroendocrinology 23 (1998) 963–87]. This definition was restricted to side-chain “shortened” steroids with 21 carbon atoms or less, and excluded sterols and their carboxylic acids with an intact side-chain. By analogy, we now use the term neurosterol to describe 27 carbon sterols synthesised in the nervous system. In this review we discuss the biological importance of neurosterols, and how they are extracted, isolated, and analysed by GC–MS and LC–MS/MS, from brain and relevant body fluids. We present applications of methodology employed for analysis of specific sterols and comment on the relative merits of the methods employed. Finally, the importance of future in-depth “sterolomic” investigations of brain is highlighted.
published_date 2009-12-31T03:22:55Z
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