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Expression and Function of Toll-Like Receptor 4 in the Endometrial Cells of the Uterus

S Herath, Martin Sheldon Orcid Logo

Endocrinology, Volume: 147, Issue: 1, Pages: 562 - 570

Swansea University Author: Martin Sheldon Orcid Logo

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DOI (Published version): 10.1210/en.2005-1113

Abstract

Prostaglandins have a central role in many endocrine functions in mammals, including regulation of the life span of the corpus luteum by prostaglandin F2α (PGF) and prostaglandin E2 (PGE), which are secreted by the uterine endometrium. However, the uterus is readily infected with bacteria such as Es...

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Published in: Endocrinology
ISSN: 0013-7227
Published: 2005
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URI: https://cronfa.swan.ac.uk/Record/cronfa11548
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spelling 2011-10-01T00:00:00.0000000 v2 11548 2012-06-14 Expression and Function of Toll-Like Receptor 4 in the Endometrial Cells of the Uterus ab0f74b794e59cc270c69e63ee1d9748 0000-0001-7902-5558 Martin Sheldon Martin Sheldon true false 2012-06-14 BMS Prostaglandins have a central role in many endocrine functions in mammals, including regulation of the life span of the corpus luteum by prostaglandin F2α (PGF) and prostaglandin E2 (PGE), which are secreted by the uterine endometrium. However, the uterus is readily infected with bacteria such as Escherichia coli, which disrupt luteolysis. Immune cells detect E. coli by Toll-like receptor 4 (TLR4) binding its pathogenic ligand, lipopolysaccharide (LPS), although signaling requires accessory molecules such as CD14. The objective of this study was to determine the effect of E. coli or LPS on the function of bovine endometrial cells, and whether purified populations of epithelial and stromal cells express the molecules involved in LPS recognition. In addition, because the female sex hormones estradiol and progesterone modify the risk of uterine infection, their effect on the LPS response was investigated. Endometrial explants produced prostaglandins in response to LPS, with an increased ratio of PGE to PGF. Addition of LPS or E. coli to stromal and epithelial cells stimulated production of PGE and PGF and increased their cyclooxygenase 2 mRNA expression. The production of prostaglandins was abrogated by an LPS antagonist. In addition, estradiol and progesterone inhibited the production of PGE and PGF in response to LPS, indicating a role for steroid hormones in the response to bacterial infection. For the first time, Toll-like receptor 4 mRNA and CD14 mRNA and protein were detected in bovine endometrial stromal and epithelial cells by RT-PCR and flow cytometry. In conclusion, epithelial and stromal cells detect and respond to bacteria, which modulate their endocrine function. Journal Article Endocrinology 147 1 562 570 0013-7227 31 12 2005 2005-12-31 10.1210/en.2005-1113 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2011-10-01T00:00:00.0000000 2012-06-14T15:38:53.7697045 Swansea University Medical School Medicine S Herath 1 Martin Sheldon 0000-0001-7902-5558 2
title Expression and Function of Toll-Like Receptor 4 in the Endometrial Cells of the Uterus
spellingShingle Expression and Function of Toll-Like Receptor 4 in the Endometrial Cells of the Uterus
Martin Sheldon
title_short Expression and Function of Toll-Like Receptor 4 in the Endometrial Cells of the Uterus
title_full Expression and Function of Toll-Like Receptor 4 in the Endometrial Cells of the Uterus
title_fullStr Expression and Function of Toll-Like Receptor 4 in the Endometrial Cells of the Uterus
title_full_unstemmed Expression and Function of Toll-Like Receptor 4 in the Endometrial Cells of the Uterus
title_sort Expression and Function of Toll-Like Receptor 4 in the Endometrial Cells of the Uterus
author_id_str_mv ab0f74b794e59cc270c69e63ee1d9748
author_id_fullname_str_mv ab0f74b794e59cc270c69e63ee1d9748_***_Martin Sheldon
author Martin Sheldon
author2 S Herath
Martin Sheldon
format Journal article
container_title Endocrinology
container_volume 147
container_issue 1
container_start_page 562
publishDate 2005
institution Swansea University
issn 0013-7227
doi_str_mv 10.1210/en.2005-1113
college_str Swansea University Medical School
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hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
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description Prostaglandins have a central role in many endocrine functions in mammals, including regulation of the life span of the corpus luteum by prostaglandin F2α (PGF) and prostaglandin E2 (PGE), which are secreted by the uterine endometrium. However, the uterus is readily infected with bacteria such as Escherichia coli, which disrupt luteolysis. Immune cells detect E. coli by Toll-like receptor 4 (TLR4) binding its pathogenic ligand, lipopolysaccharide (LPS), although signaling requires accessory molecules such as CD14. The objective of this study was to determine the effect of E. coli or LPS on the function of bovine endometrial cells, and whether purified populations of epithelial and stromal cells express the molecules involved in LPS recognition. In addition, because the female sex hormones estradiol and progesterone modify the risk of uterine infection, their effect on the LPS response was investigated. Endometrial explants produced prostaglandins in response to LPS, with an increased ratio of PGE to PGF. Addition of LPS or E. coli to stromal and epithelial cells stimulated production of PGE and PGF and increased their cyclooxygenase 2 mRNA expression. The production of prostaglandins was abrogated by an LPS antagonist. In addition, estradiol and progesterone inhibited the production of PGE and PGF in response to LPS, indicating a role for steroid hormones in the response to bacterial infection. For the first time, Toll-like receptor 4 mRNA and CD14 mRNA and protein were detected in bovine endometrial stromal and epithelial cells by RT-PCR and flow cytometry. In conclusion, epithelial and stromal cells detect and respond to bacteria, which modulate their endocrine function.
published_date 2005-12-31T03:21:19Z
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