Journal article 1048 views
Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep
M.G Gnanalingham,
M Hyatt,
J Bispham,
A Mostyn,
L Clarke,
H Budge,
M.E Symonds,
T Stephenson,
Melanie Healy
Organogenesis, Volume: 4, Issue: 3, Pages: 188 - 194
Swansea University Author: Melanie Healy
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DOI (Published version): 10.4161/org.4.3.6506
Abstract
Maternal dexamethasone administration promotes fetal maturation such that thermoregulation is improved following premature delivery and is thus comparable with a full term birth. In the present study we determined the impact of dexamethasone on both the mothers' metabolic status together with a...
Published in: | Organogenesis |
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ISSN: | 1547-6278 |
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2008
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URI: | https://cronfa.swan.ac.uk/Record/cronfa12194 |
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2011-10-01T00:00:00.0000000 v2 12194 2012-07-20 Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep 4654b4128fb21d68f98e2abc8538b45a Melanie Healy Melanie Healy true false 2012-07-20 PHAR Maternal dexamethasone administration promotes fetal maturation such that thermoregulation is improved following premature delivery and is thus comparable with a full term birth. In the present study we determined the impact of dexamethasone on both the mothers' metabolic status together with adipose tissue function in the newborn. Glucocorticoid action, adipokine gene expression and mitochondrial protein abundance were measured in perirenal adipose tissue of neonatal sheep that were born into either a warm (30 degrees C) or cool (15 degrees C) ambient temperature at 140 days of gestation (dGA; term approximately 147 dGA), either two days after maternal dexamethasone administration, or at 146 dGA for controls. Dexamethasone administration resulted in a reduction in maternal food intake in conjunction with raised plasma cortisol and free triiodothyronine. In offspring of dexamethasone administered mothers, plasma cortisol was lower and non-esterified fatty acids (NEFA) higher than controls. Glucocorticoid receptor (GR), 11beta-hydroxysteroid dehydrogenase (11beta-HSD1), interleukin-6 and uncoupling protein (UCP)1 and 2 mRNA together with voltage dependent anion channel, cytochrome c protein and UCP1 abundance were all increased by dexamethasone administration and being born into a cool ambient temperature. Gene expression of tumor necrosis factor alpha, adiponectin and peroxisome proliferator-activated receptor transcription factor gamma were unaffected by dexamethasone. The abundance of mRNA for the GR, 11beta-HSD1, UCP1 and 2 mRNA together with each protein were positively correlated to plasma NEFA and negatively correlated to plasma cortisol. In conclusion, despite reduced maternal food intake dexamethasone promotes maturation of glucocorticoid action and mitochondrial protein abundance in the newborn, an adaptation dependent on delivery temperature. Journal Article Organogenesis 4 3 188 194 1547-6278 30 9 2008 2008-09-30 10.4161/org.4.3.6506 http://www.landesbioscience.com/journals/organogenesis/article/6506/?nocache=116917466 COLLEGE NANME Pharmacy COLLEGE CODE PHAR Swansea University 2011-10-01T00:00:00.0000000 2012-07-20T11:43:25.2616738 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine M.G Gnanalingham 1 M Hyatt 2 J Bispham 3 A Mostyn 4 L Clarke 5 H Budge 6 M.E Symonds 7 T Stephenson 8 Melanie Healy 9 |
title |
Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep |
spellingShingle |
Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep Melanie Healy |
title_short |
Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep |
title_full |
Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep |
title_fullStr |
Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep |
title_full_unstemmed |
Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep |
title_sort |
Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep |
author_id_str_mv |
4654b4128fb21d68f98e2abc8538b45a |
author_id_fullname_str_mv |
4654b4128fb21d68f98e2abc8538b45a_***_Melanie Healy |
author |
Melanie Healy |
author2 |
M.G Gnanalingham M Hyatt J Bispham A Mostyn L Clarke H Budge M.E Symonds T Stephenson Melanie Healy |
format |
Journal article |
container_title |
Organogenesis |
container_volume |
4 |
container_issue |
3 |
container_start_page |
188 |
publishDate |
2008 |
institution |
Swansea University |
issn |
1547-6278 |
doi_str_mv |
10.4161/org.4.3.6506 |
college_str |
Faculty of Medicine, Health and Life Sciences |
hierarchytype |
|
hierarchy_top_id |
facultyofmedicinehealthandlifesciences |
hierarchy_top_title |
Faculty of Medicine, Health and Life Sciences |
hierarchy_parent_id |
facultyofmedicinehealthandlifesciences |
hierarchy_parent_title |
Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
url |
http://www.landesbioscience.com/journals/organogenesis/article/6506/?nocache=116917466 |
document_store_str |
0 |
active_str |
0 |
description |
Maternal dexamethasone administration promotes fetal maturation such that thermoregulation is improved following premature delivery and is thus comparable with a full term birth. In the present study we determined the impact of dexamethasone on both the mothers' metabolic status together with adipose tissue function in the newborn. Glucocorticoid action, adipokine gene expression and mitochondrial protein abundance were measured in perirenal adipose tissue of neonatal sheep that were born into either a warm (30 degrees C) or cool (15 degrees C) ambient temperature at 140 days of gestation (dGA; term approximately 147 dGA), either two days after maternal dexamethasone administration, or at 146 dGA for controls. Dexamethasone administration resulted in a reduction in maternal food intake in conjunction with raised plasma cortisol and free triiodothyronine. In offspring of dexamethasone administered mothers, plasma cortisol was lower and non-esterified fatty acids (NEFA) higher than controls. Glucocorticoid receptor (GR), 11beta-hydroxysteroid dehydrogenase (11beta-HSD1), interleukin-6 and uncoupling protein (UCP)1 and 2 mRNA together with voltage dependent anion channel, cytochrome c protein and UCP1 abundance were all increased by dexamethasone administration and being born into a cool ambient temperature. Gene expression of tumor necrosis factor alpha, adiponectin and peroxisome proliferator-activated receptor transcription factor gamma were unaffected by dexamethasone. The abundance of mRNA for the GR, 11beta-HSD1, UCP1 and 2 mRNA together with each protein were positively correlated to plasma NEFA and negatively correlated to plasma cortisol. In conclusion, despite reduced maternal food intake dexamethasone promotes maturation of glucocorticoid action and mitochondrial protein abundance in the newborn, an adaptation dependent on delivery temperature. |
published_date |
2008-09-30T03:14:06Z |
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1763750179168583680 |
score |
11.017797 |