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Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep

M.G Gnanalingham, M Hyatt, J Bispham, A Mostyn, L Clarke, H Budge, M.E Symonds, T Stephenson, Melanie Healy

Organogenesis, Volume: 4, Issue: 3, Pages: 188 - 194

Swansea University Author: Melanie Healy

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DOI (Published version): 10.4161/org.4.3.6506

Abstract

Maternal dexamethasone administration promotes fetal maturation such that thermoregulation is improved following premature delivery and is thus comparable with a full term birth. In the present study we determined the impact of dexamethasone on both the mothers' metabolic status together with a...

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Published in: Organogenesis
ISSN: 1547-6278
Published: 2008
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URI: https://cronfa.swan.ac.uk/Record/cronfa12194
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spelling 2011-10-01T00:00:00.0000000 v2 12194 2012-07-20 Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep 4654b4128fb21d68f98e2abc8538b45a Melanie Healy Melanie Healy true false 2012-07-20 BMS Maternal dexamethasone administration promotes fetal maturation such that thermoregulation is improved following premature delivery and is thus comparable with a full term birth. In the present study we determined the impact of dexamethasone on both the mothers' metabolic status together with adipose tissue function in the newborn. Glucocorticoid action, adipokine gene expression and mitochondrial protein abundance were measured in perirenal adipose tissue of neonatal sheep that were born into either a warm (30 degrees C) or cool (15 degrees C) ambient temperature at 140 days of gestation (dGA; term approximately 147 dGA), either two days after maternal dexamethasone administration, or at 146 dGA for controls. Dexamethasone administration resulted in a reduction in maternal food intake in conjunction with raised plasma cortisol and free triiodothyronine. In offspring of dexamethasone administered mothers, plasma cortisol was lower and non-esterified fatty acids (NEFA) higher than controls. Glucocorticoid receptor (GR), 11beta-hydroxysteroid dehydrogenase (11beta-HSD1), interleukin-6 and uncoupling protein (UCP)1 and 2 mRNA together with voltage dependent anion channel, cytochrome c protein and UCP1 abundance were all increased by dexamethasone administration and being born into a cool ambient temperature. Gene expression of tumor necrosis factor alpha, adiponectin and peroxisome proliferator-activated receptor transcription factor gamma were unaffected by dexamethasone. The abundance of mRNA for the GR, 11beta-HSD1, UCP1 and 2 mRNA together with each protein were positively correlated to plasma NEFA and negatively correlated to plasma cortisol. In conclusion, despite reduced maternal food intake dexamethasone promotes maturation of glucocorticoid action and mitochondrial protein abundance in the newborn, an adaptation dependent on delivery temperature. Journal Article Organogenesis 4 3 188 194 1547-6278 30 9 2008 2008-09-30 10.4161/org.4.3.6506 http://www.landesbioscience.com/journals/organogenesis/article/6506/?nocache=116917466 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2011-10-01T00:00:00.0000000 2012-07-20T11:43:25.2616738 Swansea University Medical School Medicine M.G Gnanalingham 1 M Hyatt 2 J Bispham 3 A Mostyn 4 L Clarke 5 H Budge 6 M.E Symonds 7 T Stephenson 8 Melanie Healy 9
title Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep
spellingShingle Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep
Melanie Healy
title_short Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep
title_full Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep
title_fullStr Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep
title_full_unstemmed Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep
title_sort Maternal dexamethasone administration and the maturation of perirenal adipose tissue of the neonatal sheep
author_id_str_mv 4654b4128fb21d68f98e2abc8538b45a
author_id_fullname_str_mv 4654b4128fb21d68f98e2abc8538b45a_***_Melanie Healy
author Melanie Healy
author2 M.G Gnanalingham
M Hyatt
J Bispham
A Mostyn
L Clarke
H Budge
M.E Symonds
T Stephenson
Melanie Healy
format Journal article
container_title Organogenesis
container_volume 4
container_issue 3
container_start_page 188
publishDate 2008
institution Swansea University
issn 1547-6278
doi_str_mv 10.4161/org.4.3.6506
college_str Swansea University Medical School
hierarchytype
hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
url http://www.landesbioscience.com/journals/organogenesis/article/6506/?nocache=116917466
document_store_str 0
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description Maternal dexamethasone administration promotes fetal maturation such that thermoregulation is improved following premature delivery and is thus comparable with a full term birth. In the present study we determined the impact of dexamethasone on both the mothers' metabolic status together with adipose tissue function in the newborn. Glucocorticoid action, adipokine gene expression and mitochondrial protein abundance were measured in perirenal adipose tissue of neonatal sheep that were born into either a warm (30 degrees C) or cool (15 degrees C) ambient temperature at 140 days of gestation (dGA; term approximately 147 dGA), either two days after maternal dexamethasone administration, or at 146 dGA for controls. Dexamethasone administration resulted in a reduction in maternal food intake in conjunction with raised plasma cortisol and free triiodothyronine. In offspring of dexamethasone administered mothers, plasma cortisol was lower and non-esterified fatty acids (NEFA) higher than controls. Glucocorticoid receptor (GR), 11beta-hydroxysteroid dehydrogenase (11beta-HSD1), interleukin-6 and uncoupling protein (UCP)1 and 2 mRNA together with voltage dependent anion channel, cytochrome c protein and UCP1 abundance were all increased by dexamethasone administration and being born into a cool ambient temperature. Gene expression of tumor necrosis factor alpha, adiponectin and peroxisome proliferator-activated receptor transcription factor gamma were unaffected by dexamethasone. The abundance of mRNA for the GR, 11beta-HSD1, UCP1 and 2 mRNA together with each protein were positively correlated to plasma NEFA and negatively correlated to plasma cortisol. In conclusion, despite reduced maternal food intake dexamethasone promotes maturation of glucocorticoid action and mitochondrial protein abundance in the newborn, an adaptation dependent on delivery temperature.
published_date 2008-09-30T03:28:30Z
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