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Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase.

Wendy Francis Orcid Logo, OG Bodger, I Pallister

Br J Surg, Volume: 99, Issue: 11, Pages: 1591 - 1599

Swansea University Author: Wendy Francis Orcid Logo

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DOI (Published version): 10.1002/bjs.8919

Abstract

AbstractBACKGROUND: Changes in human bone marrow associated with the systemic inflammatory response to injury are little understood. It was hypothesized that major trauma results in an altered bone marrow leucocyte progenitor profile, with either uniform depletion or the balance between multipotent...

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Published in: Br J Surg
Published: inWiley Online Library 2012
URI: https://cronfa.swan.ac.uk/Record/cronfa13008
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fullrecord <?xml version="1.0"?><rfc1807><datestamp>2011-10-01T00:00:00.0000000</datestamp><bib-version>v2</bib-version><id>13008</id><entry>2012-10-04</entry><title>Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase.</title><swanseaauthors><author><sid>f0ec2a3fdae1cf112579d579afbe9813</sid><ORCID>0000-0002-7952-2770</ORCID><firstname>Wendy</firstname><surname>Francis</surname><name>Wendy Francis</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2012-10-04</date><deptcode>BMS</deptcode><abstract>AbstractBACKGROUND: Changes in human bone marrow associated with the systemic inflammatory response to injury are little understood. It was hypothesized that major trauma results in an altered bone marrow leucocyte progenitor profile, with either uniform depletion or the balance between multipotent and committed progenitors varying, depending on whether self-renewal is favoured over differentiation.METHODS: Bone marrow aspirate and peripheral blood samples were obtained at definitive surgery in adults with pelvic fractures from blunt trauma (major trauma with Injury Severity Score (ISS) at least 18, or isolated fractures) and control patients undergoing iliac crest bone grafting. ISS, interval to surgery and transfusion in the first 24 h were recorded. Bone marrow aspirate flow cytometry was used to identify haemopoietic progenitor cells (CD34(+) ), multipotent cells (CD34(+) CD45(+) CD38(-) ) and oligopotent cells (CD34(+) CD45(+) CD38(lo/+) and CD34(+) CD45(+) CD38(BRIGHT(++ +)) subsets). Peripheral blood levels of inflammatory markers were measured, and the ratio of immature to mature (CD35(-) /CD35(+) ) granulocytes was determined.RESULTS: The median (range) interval between injury and sampling was 7 (1-21) and 5 (1-21) days in the major trauma and isolated fracture groups respectively. The CD34(+) pool was significantly depleted in the major trauma group (P = 0&#xB7;017), particularly the CD34(+) CD45(+) CD38(BRIGHT(++ +)) oligopotent pool (P = 0&#xB7;003). Immature CD35(-) granulocytes increased in bone marrow with increasing injury severity (P = 0&#xB7;024) and massive transfusion (P = 0&#xB7;019), and in peripheral blood with increasing interval to surgery (P = 0&#xB7;005).CONCLUSION: Major blunt trauma resulted in changes in the bone marrow CD34(+) progenitor pool. At the point in recovery when these samples were obtained, oligopotent progenitors were lost from the bone marrow, with continued release of immature cells. Copyright &#xA9; 2012 British Journal of Surgery Society Ltd. Published by John Wiley &amp; Sons, Ltd.</abstract><type>Journal Article</type><journal>Br J Surg</journal><volume>99</volume><journalNumber>11</journalNumber><paginationStart>1591</paginationStart><paginationEnd>1599</paginationEnd><publisher>inWiley Online Library</publisher><placeOfPublication/><issnPrint/><issnElectronic/><keywords/><publishedDay>1</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2012</publishedYear><publishedDate>2012-10-01</publishedDate><doi>10.1002/bjs.8919</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2011-10-01T00:00:00.0000000</lastEdited><Created>2012-10-04T13:52:33.0095419</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Wendy</firstname><surname>Francis</surname><orcid>0000-0002-7952-2770</orcid><order>1</order></author><author><firstname>OG</firstname><surname>Bodger</surname><order>2</order></author><author><firstname>I</firstname><surname>Pallister</surname><order>3</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2011-10-01T00:00:00.0000000 v2 13008 2012-10-04 Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase. f0ec2a3fdae1cf112579d579afbe9813 0000-0002-7952-2770 Wendy Francis Wendy Francis true false 2012-10-04 BMS AbstractBACKGROUND: Changes in human bone marrow associated with the systemic inflammatory response to injury are little understood. It was hypothesized that major trauma results in an altered bone marrow leucocyte progenitor profile, with either uniform depletion or the balance between multipotent and committed progenitors varying, depending on whether self-renewal is favoured over differentiation.METHODS: Bone marrow aspirate and peripheral blood samples were obtained at definitive surgery in adults with pelvic fractures from blunt trauma (major trauma with Injury Severity Score (ISS) at least 18, or isolated fractures) and control patients undergoing iliac crest bone grafting. ISS, interval to surgery and transfusion in the first 24 h were recorded. Bone marrow aspirate flow cytometry was used to identify haemopoietic progenitor cells (CD34(+) ), multipotent cells (CD34(+) CD45(+) CD38(-) ) and oligopotent cells (CD34(+) CD45(+) CD38(lo/+) and CD34(+) CD45(+) CD38(BRIGHT(++ +)) subsets). Peripheral blood levels of inflammatory markers were measured, and the ratio of immature to mature (CD35(-) /CD35(+) ) granulocytes was determined.RESULTS: The median (range) interval between injury and sampling was 7 (1-21) and 5 (1-21) days in the major trauma and isolated fracture groups respectively. The CD34(+) pool was significantly depleted in the major trauma group (P = 0·017), particularly the CD34(+) CD45(+) CD38(BRIGHT(++ +)) oligopotent pool (P = 0·003). Immature CD35(-) granulocytes increased in bone marrow with increasing injury severity (P = 0·024) and massive transfusion (P = 0·019), and in peripheral blood with increasing interval to surgery (P = 0·005).CONCLUSION: Major blunt trauma resulted in changes in the bone marrow CD34(+) progenitor pool. At the point in recovery when these samples were obtained, oligopotent progenitors were lost from the bone marrow, with continued release of immature cells. Copyright © 2012 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd. Journal Article Br J Surg 99 11 1591 1599 inWiley Online Library 1 10 2012 2012-10-01 10.1002/bjs.8919 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2011-10-01T00:00:00.0000000 2012-10-04T13:52:33.0095419 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Wendy Francis 0000-0002-7952-2770 1 OG Bodger 2 I Pallister 3
title Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase.
spellingShingle Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase.
Wendy Francis
title_short Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase.
title_full Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase.
title_fullStr Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase.
title_full_unstemmed Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase.
title_sort Altered leucocyte progenitor profile in human bone marrow from patients with major trauma during the recovery phase.
author_id_str_mv f0ec2a3fdae1cf112579d579afbe9813
author_id_fullname_str_mv f0ec2a3fdae1cf112579d579afbe9813_***_Wendy Francis
author Wendy Francis
author2 Wendy Francis
OG Bodger
I Pallister
format Journal article
container_title Br J Surg
container_volume 99
container_issue 11
container_start_page 1591
publishDate 2012
institution Swansea University
doi_str_mv 10.1002/bjs.8919
publisher inWiley Online Library
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description AbstractBACKGROUND: Changes in human bone marrow associated with the systemic inflammatory response to injury are little understood. It was hypothesized that major trauma results in an altered bone marrow leucocyte progenitor profile, with either uniform depletion or the balance between multipotent and committed progenitors varying, depending on whether self-renewal is favoured over differentiation.METHODS: Bone marrow aspirate and peripheral blood samples were obtained at definitive surgery in adults with pelvic fractures from blunt trauma (major trauma with Injury Severity Score (ISS) at least 18, or isolated fractures) and control patients undergoing iliac crest bone grafting. ISS, interval to surgery and transfusion in the first 24 h were recorded. Bone marrow aspirate flow cytometry was used to identify haemopoietic progenitor cells (CD34(+) ), multipotent cells (CD34(+) CD45(+) CD38(-) ) and oligopotent cells (CD34(+) CD45(+) CD38(lo/+) and CD34(+) CD45(+) CD38(BRIGHT(++ +)) subsets). Peripheral blood levels of inflammatory markers were measured, and the ratio of immature to mature (CD35(-) /CD35(+) ) granulocytes was determined.RESULTS: The median (range) interval between injury and sampling was 7 (1-21) and 5 (1-21) days in the major trauma and isolated fracture groups respectively. The CD34(+) pool was significantly depleted in the major trauma group (P = 0·017), particularly the CD34(+) CD45(+) CD38(BRIGHT(++ +)) oligopotent pool (P = 0·003). Immature CD35(-) granulocytes increased in bone marrow with increasing injury severity (P = 0·024) and massive transfusion (P = 0·019), and in peripheral blood with increasing interval to surgery (P = 0·005).CONCLUSION: Major blunt trauma resulted in changes in the bone marrow CD34(+) progenitor pool. At the point in recovery when these samples were obtained, oligopotent progenitors were lost from the bone marrow, with continued release of immature cells. Copyright © 2012 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.
published_date 2012-10-01T03:14:55Z
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score 11.016235

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