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Methods for oxysterol analysis: Past, present and future

William Griffiths Orcid Logo, Peter Crick, Yuqin Wang Orcid Logo

Biochemical Pharmacology

Swansea University Authors: William Griffiths Orcid Logo, Peter Crick, Yuqin Wang Orcid Logo

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Abstract

Oxysterols are oxidised forms of cholesterol or its precursors. In this article we will concentrate specifically on those formed in mammalian systems. Oxidation may be catalysed by endogenous enzymes or through reactive oxygen species forming a myriad of potential products. A number of these product...

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Published in: Biochemical Pharmacology
ISSN: 0006-2952
Published: 2013
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URI: https://cronfa.swan.ac.uk/Record/cronfa14484
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first_indexed 2013-07-23T12:12:18Z
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spelling 2011-10-01T00:00:00.0000000 v2 14484 2013-03-26 Methods for oxysterol analysis: Past, present and future 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 9e8253a728dc2ad7303ee8928fc85560 Peter Crick Peter Crick true false c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 2013-03-26 BMS Oxysterols are oxidised forms of cholesterol or its precursors. In this article we will concentrate specifically on those formed in mammalian systems. Oxidation may be catalysed by endogenous enzymes or through reactive oxygen species forming a myriad of potential products. A number of these products are biologically active, and oxysterols may have roles in cholesterol homeostasis, neurogenesis, protein prenylation and in the immune system. Oxysterols are also implicated in aetiology of disease states including atherosclerosis, neurodegenerative and inflammatory diseases. Reports indicating the levels of oxysterols in plasma, cerebrospinal fluid and various tissues are in many cases unrealistic owing to a lack of attention to the possibility of autoxidation, a process by which oxysterols are formed from cholesterol by oxygen in air. This article comprises a critical assessment of the technical difficulties of oxysterol analysis, highlights methodologies utilising best practise and discusses newer procedures Journal Article Biochemical Pharmacology 0006-2952 31 12 2013 2013-12-31 10.1016/j.bcp.2013.01.027 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2011-10-01T00:00:00.0000000 2013-03-26T13:48:10.5209749 Swansea University Medical School Medicine William Griffiths 0000-0002-4129-6616 1 Peter Crick 2 Yuqin Wang 0000-0002-3063-3066 3
title Methods for oxysterol analysis: Past, present and future
spellingShingle Methods for oxysterol analysis: Past, present and future
William Griffiths
Peter Crick
Yuqin Wang
title_short Methods for oxysterol analysis: Past, present and future
title_full Methods for oxysterol analysis: Past, present and future
title_fullStr Methods for oxysterol analysis: Past, present and future
title_full_unstemmed Methods for oxysterol analysis: Past, present and future
title_sort Methods for oxysterol analysis: Past, present and future
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
9e8253a728dc2ad7303ee8928fc85560
c92729b58622f9fdf6a0e7d8f4ce5081
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
9e8253a728dc2ad7303ee8928fc85560_***_Peter Crick
c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang
author William Griffiths
Peter Crick
Yuqin Wang
author2 William Griffiths
Peter Crick
Yuqin Wang
format Journal article
container_title Biochemical Pharmacology
publishDate 2013
institution Swansea University
issn 0006-2952
doi_str_mv 10.1016/j.bcp.2013.01.027
college_str Swansea University Medical School
hierarchytype
hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 0
active_str 0
description Oxysterols are oxidised forms of cholesterol or its precursors. In this article we will concentrate specifically on those formed in mammalian systems. Oxidation may be catalysed by endogenous enzymes or through reactive oxygen species forming a myriad of potential products. A number of these products are biologically active, and oxysterols may have roles in cholesterol homeostasis, neurogenesis, protein prenylation and in the immune system. Oxysterols are also implicated in aetiology of disease states including atherosclerosis, neurodegenerative and inflammatory diseases. Reports indicating the levels of oxysterols in plasma, cerebrospinal fluid and various tissues are in many cases unrealistic owing to a lack of attention to the possibility of autoxidation, a process by which oxysterols are formed from cholesterol by oxygen in air. This article comprises a critical assessment of the technical difficulties of oxysterol analysis, highlights methodologies utilising best practise and discusses newer procedures
published_date 2013-12-31T03:24:07Z
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score 10.898726