Journal article 1363 views
Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation
Paula Row,
Barbara J. REAVES,
Jan DOMIN,
J. Paul LUZIO,
Howard W. DAVIDSON
Biochemical Journal, Volume: 353, Issue: 3, Pages: 655 - 661
Swansea University Author: Paula Row
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1042/bj3530655
Abstract
Lipid kinases and their phosphorylated products are important regulators of many cellular processes, including intracellular membrane traffic. The best example of this is provided by the class III phosphoinositide 3-kinase (PI-3K), Vps34p, which is required for correct targeting of newly synthesized...
| Published in: | Biochemical Journal |
|---|---|
| ISSN: | 0264-6021 1470-8728 |
| Published: |
2001
|
| Online Access: |
Check full text
|
| URI: | https://cronfa.swan.ac.uk/Record/cronfa18374 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| first_indexed |
2014-09-11T01:59:10Z |
|---|---|
| last_indexed |
2019-06-24T19:28:50Z |
| id |
cronfa18374 |
| recordtype |
SURis |
| fullrecord |
<?xml version="1.0"?><rfc1807><datestamp>2019-06-24T15:16:24.6678513</datestamp><bib-version>v2</bib-version><id>18374</id><entry>2014-09-10</entry><title>Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation</title><swanseaauthors><author><sid>99bb528b2f8fb62aabbdad101d53ba96</sid><firstname>Paula</firstname><surname>Row</surname><name>Paula Row</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2014-09-10</date><deptcode>BMS</deptcode><abstract>Lipid kinases and their phosphorylated products are important regulators of many cellular processes, including intracellular membrane traffic. The best example of this is provided by the class III phosphoinositide 3-kinase (PI-3K), Vps34p, which is required for correct targeting of newly synthesized carboxy- peptidase Y to the yeast vacuole. A probable mammalian Vps34p orthologue has been previously identified, but its function in the trafficking of lysosomal enzymes has not been resolved. To investigate the possible role(s) of mammalian Vps34p in protein targeting to lysosomes, we have cloned the rat orthologue and overexpressed a kinase-deficient mutant in HeLa cells. Expression of the mutant protein inhibited both maturation of procathepsin D and basal secretion of the precursor. In contrast wortmannin,INTRODUCTIONThere is now abundant evidence to indicate that, in addition to their roles in cell signalling, lipid kinases and their phosphorylated products are important regulators of intracellular membrane traffic (reviewed in [1–3]). Perhaps the best example of this is provided by the class III phosphoinositide 3-kinase (PI-3K), Vps34p. This enzyme was first identified as a component of the yeast vacuolar protein sorting machinery, which, when inacti- vated, causes aberrant secretion of newly synthesized carboxy- peptidase Y (CPY) from the yeast Golgi [4,5]. In addition to its role in biosynthetic traffic, there is also considerable evidence to support a requirement for Vps34p in the endocytic pathway. For example, yeast expressing end12 (a mutant allele of VPS34) are defective in transferring endocytosed α-factor to the vacuole [6], and Vac1p\Vps19p, which is required for fusion of transport vesicles to yeast endosomes, binds the lipid product of Vps34p [7,8]. Vac1p is one member of a family of proteins that contain the FYVE domain [3], which is implicated in PtdIns(3)P binding [9,10]. Other yeast family members include Vps27p and the PtdIns(3)P 5-kinase Fab1p. Deletion of FAB1 causes abnormally large vacuoles containing fewer internal vesicles than normal to form, suggesting that the lipid product is required for correct sorting in the yeast multivesicular body [11]. Vps34p is the sole PI-3K in Saccharomyces cereisiae [5], which implies that this enzyme has multiple roles throughout the yeast endocytic system.In general, the membrane trafficking machinery appears highly conserved between all eukaryotes. Hence the involvement of a Vps34p orthologue in targeting newly synthesized lysosomal hydrolases has been postulated on the basis that PI-3K inhibitors,which also inhibited maturation, caused hypersecretion of the precursor. We propose that mammalian Vps34p plays a direct role in targeting lysosomal enzyme precursors to the endocytic pathway in an analogous fashion to its role in the fusion of early endocytic vesicles with endosomes. We further suggest that inhibition of a wortmannin-sensitive enzyme, other than mam- malian Vps34p, is responsible for the failure to recycle unoccupied mannose 6-phosphate receptors to the trans-Golgi network, and consequent hypersecretion of lysosomal enzyme precursors observed in the presence of this drug.</abstract><type>Journal Article</type><journal>Biochemical Journal</journal><volume>353</volume><journalNumber>3</journalNumber><paginationStart>655</paginationStart><paginationEnd>661</paginationEnd><publisher/><issnPrint>0264-6021</issnPrint><issnElectronic>1470-8728</issnElectronic><keywords>endosome,lysosome,phosphoinositide3-kinase, trans-Golgi network, wortmannin.</keywords><publishedDay>1</publishedDay><publishedMonth>2</publishedMonth><publishedYear>2001</publishedYear><publishedDate>2001-02-01</publishedDate><doi>10.1042/bj3530655</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2019-06-24T15:16:24.6678513</lastEdited><Created>2014-09-10T13:16:40.1042787</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Paula</firstname><surname>Row</surname><order>1</order></author><author><firstname>Barbara J.</firstname><surname>REAVES</surname><order>2</order></author><author><firstname>Jan</firstname><surname>DOMIN</surname><order>3</order></author><author><firstname>J. Paul</firstname><surname>LUZIO</surname><order>4</order></author><author><firstname>Howard W.</firstname><surname>DAVIDSON</surname><order>5</order></author></authors><documents/><OutputDurs/></rfc1807> |
| spelling |
2019-06-24T15:16:24.6678513 v2 18374 2014-09-10 Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation 99bb528b2f8fb62aabbdad101d53ba96 Paula Row Paula Row true false 2014-09-10 BMS Lipid kinases and their phosphorylated products are important regulators of many cellular processes, including intracellular membrane traffic. The best example of this is provided by the class III phosphoinositide 3-kinase (PI-3K), Vps34p, which is required for correct targeting of newly synthesized carboxy- peptidase Y to the yeast vacuole. A probable mammalian Vps34p orthologue has been previously identified, but its function in the trafficking of lysosomal enzymes has not been resolved. To investigate the possible role(s) of mammalian Vps34p in protein targeting to lysosomes, we have cloned the rat orthologue and overexpressed a kinase-deficient mutant in HeLa cells. Expression of the mutant protein inhibited both maturation of procathepsin D and basal secretion of the precursor. In contrast wortmannin,INTRODUCTIONThere is now abundant evidence to indicate that, in addition to their roles in cell signalling, lipid kinases and their phosphorylated products are important regulators of intracellular membrane traffic (reviewed in [1–3]). Perhaps the best example of this is provided by the class III phosphoinositide 3-kinase (PI-3K), Vps34p. This enzyme was first identified as a component of the yeast vacuolar protein sorting machinery, which, when inacti- vated, causes aberrant secretion of newly synthesized carboxy- peptidase Y (CPY) from the yeast Golgi [4,5]. In addition to its role in biosynthetic traffic, there is also considerable evidence to support a requirement for Vps34p in the endocytic pathway. For example, yeast expressing end12 (a mutant allele of VPS34) are defective in transferring endocytosed α-factor to the vacuole [6], and Vac1p\Vps19p, which is required for fusion of transport vesicles to yeast endosomes, binds the lipid product of Vps34p [7,8]. Vac1p is one member of a family of proteins that contain the FYVE domain [3], which is implicated in PtdIns(3)P binding [9,10]. Other yeast family members include Vps27p and the PtdIns(3)P 5-kinase Fab1p. Deletion of FAB1 causes abnormally large vacuoles containing fewer internal vesicles than normal to form, suggesting that the lipid product is required for correct sorting in the yeast multivesicular body [11]. Vps34p is the sole PI-3K in Saccharomyces cereisiae [5], which implies that this enzyme has multiple roles throughout the yeast endocytic system.In general, the membrane trafficking machinery appears highly conserved between all eukaryotes. Hence the involvement of a Vps34p orthologue in targeting newly synthesized lysosomal hydrolases has been postulated on the basis that PI-3K inhibitors,which also inhibited maturation, caused hypersecretion of the precursor. We propose that mammalian Vps34p plays a direct role in targeting lysosomal enzyme precursors to the endocytic pathway in an analogous fashion to its role in the fusion of early endocytic vesicles with endosomes. We further suggest that inhibition of a wortmannin-sensitive enzyme, other than mam- malian Vps34p, is responsible for the failure to recycle unoccupied mannose 6-phosphate receptors to the trans-Golgi network, and consequent hypersecretion of lysosomal enzyme precursors observed in the presence of this drug. Journal Article Biochemical Journal 353 3 655 661 0264-6021 1470-8728 endosome,lysosome,phosphoinositide3-kinase, trans-Golgi network, wortmannin. 1 2 2001 2001-02-01 10.1042/bj3530655 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2019-06-24T15:16:24.6678513 2014-09-10T13:16:40.1042787 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Paula Row 1 Barbara J. REAVES 2 Jan DOMIN 3 J. Paul LUZIO 4 Howard W. DAVIDSON 5 |
| title |
Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation |
| spellingShingle |
Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation Paula Row |
| title_short |
Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation |
| title_full |
Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation |
| title_fullStr |
Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation |
| title_full_unstemmed |
Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation |
| title_sort |
Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation |
| author_id_str_mv |
99bb528b2f8fb62aabbdad101d53ba96 |
| author_id_fullname_str_mv |
99bb528b2f8fb62aabbdad101d53ba96_***_Paula Row |
| author |
Paula Row |
| author2 |
Paula Row Barbara J. REAVES Jan DOMIN J. Paul LUZIO Howard W. DAVIDSON |
| format |
Journal article |
| container_title |
Biochemical Journal |
| container_volume |
353 |
| container_issue |
3 |
| container_start_page |
655 |
| publishDate |
2001 |
| institution |
Swansea University |
| issn |
0264-6021 1470-8728 |
| doi_str_mv |
10.1042/bj3530655 |
| college_str |
Faculty of Medicine, Health and Life Sciences |
| hierarchytype |
|
| hierarchy_top_id |
facultyofmedicinehealthandlifesciences |
| hierarchy_top_title |
Faculty of Medicine, Health and Life Sciences |
| hierarchy_parent_id |
facultyofmedicinehealthandlifesciences |
| hierarchy_parent_title |
Faculty of Medicine, Health and Life Sciences |
| department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
| document_store_str |
0 |
| active_str |
0 |
| description |
Lipid kinases and their phosphorylated products are important regulators of many cellular processes, including intracellular membrane traffic. The best example of this is provided by the class III phosphoinositide 3-kinase (PI-3K), Vps34p, which is required for correct targeting of newly synthesized carboxy- peptidase Y to the yeast vacuole. A probable mammalian Vps34p orthologue has been previously identified, but its function in the trafficking of lysosomal enzymes has not been resolved. To investigate the possible role(s) of mammalian Vps34p in protein targeting to lysosomes, we have cloned the rat orthologue and overexpressed a kinase-deficient mutant in HeLa cells. Expression of the mutant protein inhibited both maturation of procathepsin D and basal secretion of the precursor. In contrast wortmannin,INTRODUCTIONThere is now abundant evidence to indicate that, in addition to their roles in cell signalling, lipid kinases and their phosphorylated products are important regulators of intracellular membrane traffic (reviewed in [1–3]). Perhaps the best example of this is provided by the class III phosphoinositide 3-kinase (PI-3K), Vps34p. This enzyme was first identified as a component of the yeast vacuolar protein sorting machinery, which, when inacti- vated, causes aberrant secretion of newly synthesized carboxy- peptidase Y (CPY) from the yeast Golgi [4,5]. In addition to its role in biosynthetic traffic, there is also considerable evidence to support a requirement for Vps34p in the endocytic pathway. For example, yeast expressing end12 (a mutant allele of VPS34) are defective in transferring endocytosed α-factor to the vacuole [6], and Vac1p\Vps19p, which is required for fusion of transport vesicles to yeast endosomes, binds the lipid product of Vps34p [7,8]. Vac1p is one member of a family of proteins that contain the FYVE domain [3], which is implicated in PtdIns(3)P binding [9,10]. Other yeast family members include Vps27p and the PtdIns(3)P 5-kinase Fab1p. Deletion of FAB1 causes abnormally large vacuoles containing fewer internal vesicles than normal to form, suggesting that the lipid product is required for correct sorting in the yeast multivesicular body [11]. Vps34p is the sole PI-3K in Saccharomyces cereisiae [5], which implies that this enzyme has multiple roles throughout the yeast endocytic system.In general, the membrane trafficking machinery appears highly conserved between all eukaryotes. Hence the involvement of a Vps34p orthologue in targeting newly synthesized lysosomal hydrolases has been postulated on the basis that PI-3K inhibitors,which also inhibited maturation, caused hypersecretion of the precursor. We propose that mammalian Vps34p plays a direct role in targeting lysosomal enzyme precursors to the endocytic pathway in an analogous fashion to its role in the fusion of early endocytic vesicles with endosomes. We further suggest that inhibition of a wortmannin-sensitive enzyme, other than mam- malian Vps34p, is responsible for the failure to recycle unoccupied mannose 6-phosphate receptors to the trans-Golgi network, and consequent hypersecretion of lysosomal enzyme precursors observed in the presence of this drug. |
| published_date |
2001-02-01T03:21:32Z |
| _version_ |
1763750646873325568 |
| score |
11.036706 |