No Cover Image

Journal article 472 views

Lysosome Associated Membrane Protein 1 (Lamp1) Traffics Directly from the TGN to Early Endosomes / Paula, Row

Traffic, Volume: 5, Issue: 9, Pages: 685 - 699

Swansea University Author: Paula, Row

Full text not available from this repository: check for access using links below.

DOI (Published version): 10.1111/j.1600-0854.2004.00212.x

Abstract

The precise trafficking routes followed by newly synthe- sized lysosomal membrane proteins after exit from the Golgi are unclear. To study these events we created a novel chimera (YAL) having a lumenal domain compris- ing two tyrosine sulfation motifs fused to avidin, and the transmembrane and cytop...

Full description

Published in: Traffic
Published: 2004
Online Access: http://onlinelibrary.wiley.com/store/10.1111/j.1600-0854.2004.00212.x/asset/j.1600-0854.2004.00212.x.pdf?v=1&t=hzwpbpou&s=b8b7d06384e9820167203022a49642432b43aa96
URI: https://cronfa.swan.ac.uk/Record/cronfa18381
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: The precise trafficking routes followed by newly synthe- sized lysosomal membrane proteins after exit from the Golgi are unclear. To study these events we created a novel chimera (YAL) having a lumenal domain compris- ing two tyrosine sulfation motifs fused to avidin, and the transmembrane and cytoplasmic domains of lysosome associated membrane protein 1 (Lamp1). The newly synthesized protein rapidly transited from the trans- Golgi Network (TGN) to lysosomes (t1/2 ~30min after a lag of 15–20 min). However, labeled chimera was captured by biotinylated probes endocytosed for only 5 min, indicat- ing that the initial site of entry into the endocytic pathway was early endosomes. Capture required export of YAL from the TGN, and endocytosis of the biotinylated reagent, and was essentially quantitative within 2h of chase, suggesting that all molecules were following an identical route. There was no evidence of YAL trafficking via the cell surface. Fusion of TGN-derived vesicles with 5min endosomes could be recapitulated in vitro, but neither late endosomes nor lysosomes could serve as acceptor compartments. This suggests that contrary to previous conclusions, most if not all newly synthesized Lamp1 traffics from the TGN to early endosomes prior to delivery to late endosomes and lysosomes.
Keywords: avidin, endosome, Lgp120, lysosome, TGN
College: Swansea University Medical School
Issue: 9
Start Page: 685
End Page: 699