Journal article 1248 views
Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes
D.A. Jones,
S.L. Prior,
J.D. Barry,
S. Caplin,
J.N. Baxter,
J.W. Stephens,
Sarah Prior 
,
Jeffrey Stephens ,
Danielle Jones
,
Jeffrey Stephens ,
Danielle Jones
Diabetes Research and Clinical Practice, Volume: 106, Issue: 3, Pages: 627 - 633
Swansea University Authors:
Sarah Prior , Jeffrey Stephens , Danielle Jones
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1016/j.diabres.2014.09.054
Abstract
AimsIn the past 30 years, prevalence of obesity has almost trebled resulting in an increased incidence of type 2 diabetes mellitus and other co-morbidities. Visceral adipose tissue is believed to play a vital role, but underlying mechanisms remain unclear. Our aim was to investigate changes in marke...
| Published in: | Diabetes Research and Clinical Practice |
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2014
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa19724 |
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<?xml version="1.0"?><rfc1807><datestamp>2018-05-09T15:26:13.6058382</datestamp><bib-version>v2</bib-version><id>19724</id><entry>2014-12-05</entry><title>Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes</title><swanseaauthors><author><sid>cdda101035997acfaa6fdf17097f52b2</sid><ORCID>0000-0001-8703-8092</ORCID><firstname>Sarah</firstname><surname>Prior</surname><name>Sarah Prior</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>5219d126f97f8f884bdb622099bd41de</sid><ORCID>0000-0003-2228-086X</ORCID><firstname>Jeffrey</firstname><surname>Stephens</surname><name>Jeffrey Stephens</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>4d474dbca816c2c209faef443be18a66</sid><firstname>Danielle</firstname><surname>Jones</surname><name>Danielle Jones</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2014-12-05</date><deptcode>BMS</deptcode><abstract>AimsIn the past 30 years, prevalence of obesity has almost trebled resulting in an increased incidence of type 2 diabetes mellitus and other co-morbidities. Visceral adipose tissue is believed to play a vital role, but underlying mechanisms remain unclear. Our aim was to investigate changes in markers of oxidative damage in human visceral adipose tissue to determine levels of oxidative burden that may be attributed to obesity and/or diabetes.MethodsVisceral adipose tissue samples from 61 subjects undergoing abdominal surgery grouped as lean, obese and obese with type 2 diabetes mellitus, were examined using 3 different markers of oxidative stress. Malondialdehyde (MDA) concentration was measured as a marker of lipid peroxidation, telomere length and Comet assay as markers of oxidative DNA damage.ResultsNo significant difference in MDA concentration, telomere length and DNA damage was observed between groups, although longer telomere lengths were seen in the obese with diabetes group compared to the obese group (P &#60; 0.05). Lower MDA concentration and longer telomere length were seen in subjects with diabetes compared to those without (P &#60; 0.05). DNA damage, analysed via Comet assay, was significantly lower in subjects with diabetes compared to those without (P &#60; 0.05).ConclusionA paradoxical decrease in oxidative stress and DNA damage was observed in samples from subjects with type 2 diabetes mellitus. Further work is required to investigate this further, however this phenomenon may be due to an up regulation of antioxidant defences in adipose tissue.</abstract><type>Journal Article</type><journal>Diabetes Research and Clinical Practice</journal><volume>106</volume><journalNumber>3</journalNumber><paginationStart>627</paginationStart><paginationEnd>633</paginationEnd><publisher/><keywords>Keywords: Oxidative stress, Obesity, Type 2 diabetes mellitus</keywords><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2014</publishedYear><publishedDate>2014-12-31</publishedDate><doi>10.1016/j.diabres.2014.09.054</doi><url/><notes></notes><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2018-05-09T15:26:13.6058382</lastEdited><Created>2014-12-05T14:27:51.8411884</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>D.A.</firstname><surname>Jones</surname><order>1</order></author><author><firstname>S.L.</firstname><surname>Prior</surname><order>2</order></author><author><firstname>J.D.</firstname><surname>Barry</surname><order>3</order></author><author><firstname>S.</firstname><surname>Caplin</surname><order>4</order></author><author><firstname>J.N.</firstname><surname>Baxter</surname><order>5</order></author><author><firstname>J.W.</firstname><surname>Stephens</surname><order>6</order></author><author><firstname>Sarah</firstname><surname>Prior</surname><orcid>0000-0001-8703-8092</orcid><order>7</order></author><author><firstname>Jeffrey</firstname><surname>Stephens</surname><orcid>0000-0003-2228-086X</orcid><order>8</order></author><author><firstname>Danielle</firstname><surname>Jones</surname><order>9</order></author></authors><documents/><OutputDurs/></rfc1807> |
| spelling |
2018-05-09T15:26:13.6058382 v2 19724 2014-12-05 Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes cdda101035997acfaa6fdf17097f52b2 0000-0001-8703-8092 Sarah Prior Sarah Prior true false 5219d126f97f8f884bdb622099bd41de 0000-0003-2228-086X Jeffrey Stephens Jeffrey Stephens true false 4d474dbca816c2c209faef443be18a66 Danielle Jones Danielle Jones true false 2014-12-05 BMS AimsIn the past 30 years, prevalence of obesity has almost trebled resulting in an increased incidence of type 2 diabetes mellitus and other co-morbidities. Visceral adipose tissue is believed to play a vital role, but underlying mechanisms remain unclear. Our aim was to investigate changes in markers of oxidative damage in human visceral adipose tissue to determine levels of oxidative burden that may be attributed to obesity and/or diabetes.MethodsVisceral adipose tissue samples from 61 subjects undergoing abdominal surgery grouped as lean, obese and obese with type 2 diabetes mellitus, were examined using 3 different markers of oxidative stress. Malondialdehyde (MDA) concentration was measured as a marker of lipid peroxidation, telomere length and Comet assay as markers of oxidative DNA damage.ResultsNo significant difference in MDA concentration, telomere length and DNA damage was observed between groups, although longer telomere lengths were seen in the obese with diabetes group compared to the obese group (P < 0.05). Lower MDA concentration and longer telomere length were seen in subjects with diabetes compared to those without (P < 0.05). DNA damage, analysed via Comet assay, was significantly lower in subjects with diabetes compared to those without (P < 0.05).ConclusionA paradoxical decrease in oxidative stress and DNA damage was observed in samples from subjects with type 2 diabetes mellitus. Further work is required to investigate this further, however this phenomenon may be due to an up regulation of antioxidant defences in adipose tissue. Journal Article Diabetes Research and Clinical Practice 106 3 627 633 Keywords: Oxidative stress, Obesity, Type 2 diabetes mellitus 31 12 2014 2014-12-31 10.1016/j.diabres.2014.09.054 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2018-05-09T15:26:13.6058382 2014-12-05T14:27:51.8411884 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine D.A. Jones 1 S.L. Prior 2 J.D. Barry 3 S. Caplin 4 J.N. Baxter 5 J.W. Stephens 6 Sarah Prior 0000-0001-8703-8092 7 Jeffrey Stephens 0000-0003-2228-086X 8 Danielle Jones 9 |
| title |
Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes |
| spellingShingle |
Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes Sarah Prior Jeffrey Stephens Danielle Jones |
| title_short |
Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes |
| title_full |
Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes |
| title_fullStr |
Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes |
| title_full_unstemmed |
Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes |
| title_sort |
Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes |
| author_id_str_mv |
cdda101035997acfaa6fdf17097f52b2 5219d126f97f8f884bdb622099bd41de 4d474dbca816c2c209faef443be18a66 |
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cdda101035997acfaa6fdf17097f52b2_***_Sarah Prior 5219d126f97f8f884bdb622099bd41de_***_Jeffrey Stephens 4d474dbca816c2c209faef443be18a66_***_Danielle Jones |
| author |
Sarah Prior Jeffrey Stephens Danielle Jones |
| author2 |
D.A. Jones S.L. Prior J.D. Barry S. Caplin J.N. Baxter J.W. Stephens Sarah Prior Jeffrey Stephens Danielle Jones |
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Journal article |
| container_title |
Diabetes Research and Clinical Practice |
| container_volume |
106 |
| container_issue |
3 |
| container_start_page |
627 |
| publishDate |
2014 |
| institution |
Swansea University |
| doi_str_mv |
10.1016/j.diabres.2014.09.054 |
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Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
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Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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| description |
AimsIn the past 30 years, prevalence of obesity has almost trebled resulting in an increased incidence of type 2 diabetes mellitus and other co-morbidities. Visceral adipose tissue is believed to play a vital role, but underlying mechanisms remain unclear. Our aim was to investigate changes in markers of oxidative damage in human visceral adipose tissue to determine levels of oxidative burden that may be attributed to obesity and/or diabetes.MethodsVisceral adipose tissue samples from 61 subjects undergoing abdominal surgery grouped as lean, obese and obese with type 2 diabetes mellitus, were examined using 3 different markers of oxidative stress. Malondialdehyde (MDA) concentration was measured as a marker of lipid peroxidation, telomere length and Comet assay as markers of oxidative DNA damage.ResultsNo significant difference in MDA concentration, telomere length and DNA damage was observed between groups, although longer telomere lengths were seen in the obese with diabetes group compared to the obese group (P < 0.05). Lower MDA concentration and longer telomere length were seen in subjects with diabetes compared to those without (P < 0.05). DNA damage, analysed via Comet assay, was significantly lower in subjects with diabetes compared to those without (P < 0.05).ConclusionA paradoxical decrease in oxidative stress and DNA damage was observed in samples from subjects with type 2 diabetes mellitus. Further work is required to investigate this further, however this phenomenon may be due to an up regulation of antioxidant defences in adipose tissue. |
| published_date |
2014-12-31T03:23:14Z |
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1763750753674985472 |
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11.002155 |