No Cover Image

Journal article 1036 views

Cholesterol metabolites exported from human brain

Luigi Iuliano, Peter J. Crick, Chiara Zerbinati, Luigi Tritapepe, Jonas Abdel-Khalik, Marc Poirot, Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

Steroids

Swansea University Authors: Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

Full text not available from this repository: check for access using links below.

DOI (Published version): 10.1016/j.steroids.2015.01.026

Abstract

The human brain contains approximately 25% of the body's cholesterol. The brain is separated from the circulation by the blood brain barrier. While cholesterol will not passes this barrier, oxygenated forms of cholesterol can cross the barrier. Here by measuring the difference in the oxysterol...

Full description

Published in: Steroids
Published: 2015
URI: https://cronfa.swan.ac.uk/Record/cronfa20171
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: The human brain contains approximately 25% of the body's cholesterol. The brain is separated from the circulation by the blood brain barrier. While cholesterol will not passes this barrier, oxygenated forms of cholesterol can cross the barrier. Here by measuring the difference in the oxysterol content of blood plasma in the jugular vein and in a forearm vein by mass spectrometry (MS) we were able to determine the flux of more than 20 cholesterol metabolites between brain and the circulation. We confirm that 24S-hydroxycholesterol is exported from brain at a rate of about 2 - 3 mg/24 hr. Gas chromatography (GC) - MS data shows that the cholesterol metabolites 5α-hydroxy-6-oxocholesterol (3β,5α-dihydroxycholestan-6-one), 7β-hydroxycholesterol and 7-oxocholesterol, generally considered to be formed through reactive oxygen species, are similarly exported from brain at rates of about 0.1, 2 and 2 mg/24 hr, respectively. Although not to statistical significance both GC-MS and liquid chromatography (LC) - MS methods indicate that (25R)26-hydroxycholesterol is imported to brain, while LC-MS indicates that 7α-hydroxy-3-oxocholest-4-enoic acid is exported from brain.
College: Faculty of Medicine, Health and Life Sciences