Journal article 1531 views 210 downloads
Dynasore - not just a dynamin inhibitor
Cell Communication and Signaling, Volume: 13, Issue: 1
Swansea University Authors: James Cronin , Martin Sheldon
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DOI (Published version): 10.1186/s12964-015-0102-1
Abstract
Dynamin is a GTPase protein that is essential for membrane fission during clathrin-mediated endocytosis in eukaryotic cells. Dynasore is a GTPase inhibitor that rapidly and reversibly inhibits dynamin activity, which prevents endocytosis. However, comparison between cells treated with dynasore and R...
Published in: | Cell Communication and Signaling |
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ISSN: | 1478-811X |
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2015
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URI: | https://cronfa.swan.ac.uk/Record/cronfa20642 |
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2019-10-14T10:52:24.4518323 v2 20642 2015-04-13 Dynasore - not just a dynamin inhibitor 9cfd17551c0d1f7438895121e4fbb6e8 0000-0002-0590-9462 James Cronin James Cronin true false ab0f74b794e59cc270c69e63ee1d9748 0000-0001-7902-5558 Martin Sheldon Martin Sheldon true false 2015-04-13 BMS Dynamin is a GTPase protein that is essential for membrane fission during clathrin-mediated endocytosis in eukaryotic cells. Dynasore is a GTPase inhibitor that rapidly and reversibly inhibits dynamin activity, which prevents endocytosis. However, comparison between cells treated with dynasore and RNA interference of genes encoding dynamin, reveals evidence that dynasore reduces labile cholesterol in the plasma membrane, and disrupts lipid raft organization, in a dynamin-independent manner. To explore the role of dynamin it is important to use multiple dynamin inhibitors, alongside the use of dynamin mutants and RNA interference targeting genes encoding dynamin. On the other hand, dynasore provides an interesting tool to explore the regulation of cholesterol in plasma membranes. Journal Article Cell Communication and Signaling 13 1 1478-811X 13 4 2015 2015-04-13 10.1186/s12964-015-0102-1 http://www.biosignaling.com/content/pdf/s12964-015-0102-1.pdf COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2019-10-14T10:52:24.4518323 2015-04-13T19:59:53.0280192 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Giulio Preta 1 James Cronin 0000-0002-0590-9462 2 I Martin Sheldon 3 Martin Sheldon 0000-0001-7902-5558 4 0020642-13042015200106.pdf s12964-015-0102-1.pdf 2015-04-13T20:01:06.4870000 Output 1357605 application/pdf Version of Record true 2015-04-13T00:00:00.0000000 Distributed under the terms of a Creative Commons Attribution (CC-BY-4.0) true |
title |
Dynasore - not just a dynamin inhibitor |
spellingShingle |
Dynasore - not just a dynamin inhibitor James Cronin Martin Sheldon |
title_short |
Dynasore - not just a dynamin inhibitor |
title_full |
Dynasore - not just a dynamin inhibitor |
title_fullStr |
Dynasore - not just a dynamin inhibitor |
title_full_unstemmed |
Dynasore - not just a dynamin inhibitor |
title_sort |
Dynasore - not just a dynamin inhibitor |
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9cfd17551c0d1f7438895121e4fbb6e8 ab0f74b794e59cc270c69e63ee1d9748 |
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9cfd17551c0d1f7438895121e4fbb6e8_***_James Cronin ab0f74b794e59cc270c69e63ee1d9748_***_Martin Sheldon |
author |
James Cronin Martin Sheldon |
author2 |
Giulio Preta James Cronin I Martin Sheldon Martin Sheldon |
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Journal article |
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Cell Communication and Signaling |
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13 |
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2015 |
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Swansea University |
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1478-811X |
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10.1186/s12964-015-0102-1 |
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Faculty of Medicine, Health and Life Sciences |
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Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
url |
http://www.biosignaling.com/content/pdf/s12964-015-0102-1.pdf |
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description |
Dynamin is a GTPase protein that is essential for membrane fission during clathrin-mediated endocytosis in eukaryotic cells. Dynasore is a GTPase inhibitor that rapidly and reversibly inhibits dynamin activity, which prevents endocytosis. However, comparison between cells treated with dynasore and RNA interference of genes encoding dynamin, reveals evidence that dynasore reduces labile cholesterol in the plasma membrane, and disrupts lipid raft organization, in a dynamin-independent manner. To explore the role of dynamin it is important to use multiple dynamin inhibitors, alongside the use of dynamin mutants and RNA interference targeting genes encoding dynamin. On the other hand, dynasore provides an interesting tool to explore the regulation of cholesterol in plasma membranes. |
published_date |
2015-04-13T03:24:26Z |
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11.03559 |