Journal article 1877 views 562 downloads
Bioenergetic analysis of human peripheral blood mononuclear cells
Clinical & Experimental Immunology, Volume: 182, Issue: 1, Pages: 69 - 80
Swansea University Authors: Nigel Francis, David Skibinski , Ruth Jones , Cathy Thornton , Aled Bryant , Nick Jones
-
PDF | Accepted Manuscript
Download (876.03KB)
DOI (Published version): 10.1111/cei.12662
Abstract
Leukocytes respond rapidly to pathogenic and other insults with responses ranging from cytokine production through to migration and phagocytosis. These are bioenergetically expensive and increased glycolytic flux provides ATP rapidly to support these essential functions. However, much of this work i...
Published in: | Clinical & Experimental Immunology |
---|---|
Published: |
2015
|
URI: | https://cronfa.swan.ac.uk/Record/cronfa23517 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
first_indexed |
2015-10-01T02:09:34Z |
---|---|
last_indexed |
2018-02-09T05:02:24Z |
id |
cronfa23517 |
recordtype |
SURis |
fullrecord |
<?xml version="1.0"?><rfc1807><datestamp>2017-01-23T14:20:49.9550871</datestamp><bib-version>v2</bib-version><id>23517</id><entry>2015-09-30</entry><title>Bioenergetic analysis of human peripheral blood mononuclear cells</title><swanseaauthors><author><sid>a726d297bbed7e5cd4c320f8f9dbf4d7</sid><firstname>Nigel</firstname><surname>Francis</surname><name>Nigel Francis</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>328d16903f98c2b03a1cc64a7530322a</sid><ORCID>0000-0003-4077-6236</ORCID><firstname>David</firstname><surname>Skibinski</surname><name>David Skibinski</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>a1a281c8720685c422892ef168d4b279</sid><ORCID>0000-0001-5811-8827</ORCID><firstname>Ruth</firstname><surname>Jones</surname><name>Ruth Jones</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>c71a7a4be7361094d046d312202bce0c</sid><ORCID>0000-0002-5153-573X</ORCID><firstname>Cathy</firstname><surname>Thornton</surname><name>Cathy Thornton</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>021f7adc0923f6d2c2e2fc269758b8fe</sid><ORCID>0000-0002-4650-4672</ORCID><firstname>Aled</firstname><surname>Bryant</surname><name>Aled Bryant</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>0fce0f7ddbdbfeb968f4e2f1e3f86744</sid><ORCID>0000-0003-4846-5117</ORCID><firstname>Nick</firstname><surname>Jones</surname><name>Nick Jones</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2015-09-30</date><deptcode>FGMHL</deptcode><abstract>Leukocytes respond rapidly to pathogenic and other insults with responses ranging from cytokine production through to migration and phagocytosis. These are bioenergetically expensive and increased glycolytic flux provides ATP rapidly to support these essential functions. However, much of this work is from animal studies. To better understand the relative role of glycolysis and oxidative phosphorylation in human leukocytes, especially their utility in a translational research setting, we undertook a study of human peripheral blood mononuclear cells (MNCs) bioenergetics. Glycolysis was essential during LPS-mediated IL-1, IL-6, and TNF production as 2-deoxy-D-glucose significantly decreased output of all three cytokines. After optimising cell numbers and the concentrations of all activators and inhibitors, oxidative phosphorylation and glycolysis profiles of fresh and cryopreserved/resuscitated MNCs were determined to explore the utility of MNCs for determining the bioenergetics health profile in multiple clinical settings. While the LPS-induced cytokine response did not differ significantly between fresh and resuscitated cells from the same donors, cryopreservation/resuscitation significantly affected some measures of oxidative phosphorylation mostly, but also glycolysis. Bioenergetics analysis of human MNCs provides a quick, effective means to measure the bioenergetics health index of many individuals but cryopreserved cells are not suitable for such an analysis. The translational utility of this approach was tested by comparing MNCs of pregnant and non-pregnant women to reveal increased bioenergetics health index with pregnancy but significantly reduced basal glycolysis and glycolytic capacity. More detailed analysis of discrete leukocyte populations would be required to understand the relative roles of glycolysis and oxidative phosphorylation during inflammation and other immune responses.</abstract><type>Journal Article</type><journal>Clinical & Experimental Immunology</journal><volume>182</volume><journalNumber>1</journalNumber><paginationStart>69</paginationStart><paginationEnd>80</paginationEnd><publisher/><keywords/><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2015</publishedYear><publishedDate>2015-12-31</publishedDate><doi>10.1111/cei.12662</doi><url/><notes></notes><college>COLLEGE NANME</college><department>Medicine, Health and Life Science - Faculty</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>FGMHL</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2017-01-23T14:20:49.9550871</lastEdited><Created>2015-09-30T12:35:13.4112942</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>N.</firstname><surname>Jones</surname><order>1</order></author><author><firstname>J.</firstname><surname>Piasecka</surname><order>2</order></author><author><firstname>A. H.</firstname><surname>Bryant</surname><order>3</order></author><author><firstname>R. H.</firstname><surname>Jones</surname><order>4</order></author><author><firstname>D. O. F.</firstname><surname>Skibinski</surname><order>5</order></author><author><firstname>Nigel</firstname><surname>Francis</surname><order>6</order></author><author><firstname>C. A.</firstname><surname>Thornton</surname><order>7</order></author><author><firstname>David</firstname><surname>Skibinski</surname><orcid>0000-0003-4077-6236</orcid><order>8</order></author><author><firstname>Ruth</firstname><surname>Jones</surname><orcid>0000-0001-5811-8827</orcid><order>9</order></author><author><firstname>Cathy</firstname><surname>Thornton</surname><orcid>0000-0002-5153-573X</orcid><order>10</order></author><author><firstname>Aled</firstname><surname>Bryant</surname><orcid>0000-0002-4650-4672</orcid><order>11</order></author><author><firstname>Nick</firstname><surname>Jones</surname><orcid>0000-0003-4846-5117</orcid><order>12</order></author></authors><documents><document><filename>0023517-14012016094417.pdf</filename><originalFilename>JonesetalBioenergeticsofHumanPBMCsacceptedversion.pdf</originalFilename><uploaded>2016-01-14T09:44:17.2200000</uploaded><type>Output</type><contentLength>856535</contentLength><contentType>application/pdf</contentType><version>Accepted Manuscript</version><cronfaStatus>true</cronfaStatus><embargoDate>2016-07-31T00:00:00.0000000</embargoDate><documentNotes/><copyrightCorrect>true</copyrightCorrect></document></documents><OutputDurs/></rfc1807> |
spelling |
2017-01-23T14:20:49.9550871 v2 23517 2015-09-30 Bioenergetic analysis of human peripheral blood mononuclear cells a726d297bbed7e5cd4c320f8f9dbf4d7 Nigel Francis Nigel Francis true false 328d16903f98c2b03a1cc64a7530322a 0000-0003-4077-6236 David Skibinski David Skibinski true false a1a281c8720685c422892ef168d4b279 0000-0001-5811-8827 Ruth Jones Ruth Jones true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 021f7adc0923f6d2c2e2fc269758b8fe 0000-0002-4650-4672 Aled Bryant Aled Bryant true false 0fce0f7ddbdbfeb968f4e2f1e3f86744 0000-0003-4846-5117 Nick Jones Nick Jones true false 2015-09-30 FGMHL Leukocytes respond rapidly to pathogenic and other insults with responses ranging from cytokine production through to migration and phagocytosis. These are bioenergetically expensive and increased glycolytic flux provides ATP rapidly to support these essential functions. However, much of this work is from animal studies. To better understand the relative role of glycolysis and oxidative phosphorylation in human leukocytes, especially their utility in a translational research setting, we undertook a study of human peripheral blood mononuclear cells (MNCs) bioenergetics. Glycolysis was essential during LPS-mediated IL-1, IL-6, and TNF production as 2-deoxy-D-glucose significantly decreased output of all three cytokines. After optimising cell numbers and the concentrations of all activators and inhibitors, oxidative phosphorylation and glycolysis profiles of fresh and cryopreserved/resuscitated MNCs were determined to explore the utility of MNCs for determining the bioenergetics health profile in multiple clinical settings. While the LPS-induced cytokine response did not differ significantly between fresh and resuscitated cells from the same donors, cryopreservation/resuscitation significantly affected some measures of oxidative phosphorylation mostly, but also glycolysis. Bioenergetics analysis of human MNCs provides a quick, effective means to measure the bioenergetics health index of many individuals but cryopreserved cells are not suitable for such an analysis. The translational utility of this approach was tested by comparing MNCs of pregnant and non-pregnant women to reveal increased bioenergetics health index with pregnancy but significantly reduced basal glycolysis and glycolytic capacity. More detailed analysis of discrete leukocyte populations would be required to understand the relative roles of glycolysis and oxidative phosphorylation during inflammation and other immune responses. Journal Article Clinical & Experimental Immunology 182 1 69 80 31 12 2015 2015-12-31 10.1111/cei.12662 COLLEGE NANME Medicine, Health and Life Science - Faculty COLLEGE CODE FGMHL Swansea University 2017-01-23T14:20:49.9550871 2015-09-30T12:35:13.4112942 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine N. Jones 1 J. Piasecka 2 A. H. Bryant 3 R. H. Jones 4 D. O. F. Skibinski 5 Nigel Francis 6 C. A. Thornton 7 David Skibinski 0000-0003-4077-6236 8 Ruth Jones 0000-0001-5811-8827 9 Cathy Thornton 0000-0002-5153-573X 10 Aled Bryant 0000-0002-4650-4672 11 Nick Jones 0000-0003-4846-5117 12 0023517-14012016094417.pdf JonesetalBioenergeticsofHumanPBMCsacceptedversion.pdf 2016-01-14T09:44:17.2200000 Output 856535 application/pdf Accepted Manuscript true 2016-07-31T00:00:00.0000000 true |
title |
Bioenergetic analysis of human peripheral blood mononuclear cells |
spellingShingle |
Bioenergetic analysis of human peripheral blood mononuclear cells Nigel Francis David Skibinski Ruth Jones Cathy Thornton Aled Bryant Nick Jones |
title_short |
Bioenergetic analysis of human peripheral blood mononuclear cells |
title_full |
Bioenergetic analysis of human peripheral blood mononuclear cells |
title_fullStr |
Bioenergetic analysis of human peripheral blood mononuclear cells |
title_full_unstemmed |
Bioenergetic analysis of human peripheral blood mononuclear cells |
title_sort |
Bioenergetic analysis of human peripheral blood mononuclear cells |
author_id_str_mv |
a726d297bbed7e5cd4c320f8f9dbf4d7 328d16903f98c2b03a1cc64a7530322a a1a281c8720685c422892ef168d4b279 c71a7a4be7361094d046d312202bce0c 021f7adc0923f6d2c2e2fc269758b8fe 0fce0f7ddbdbfeb968f4e2f1e3f86744 |
author_id_fullname_str_mv |
a726d297bbed7e5cd4c320f8f9dbf4d7_***_Nigel Francis 328d16903f98c2b03a1cc64a7530322a_***_David Skibinski a1a281c8720685c422892ef168d4b279_***_Ruth Jones c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton 021f7adc0923f6d2c2e2fc269758b8fe_***_Aled Bryant 0fce0f7ddbdbfeb968f4e2f1e3f86744_***_Nick Jones |
author |
Nigel Francis David Skibinski Ruth Jones Cathy Thornton Aled Bryant Nick Jones |
author2 |
N. Jones J. Piasecka A. H. Bryant R. H. Jones D. O. F. Skibinski Nigel Francis C. A. Thornton David Skibinski Ruth Jones Cathy Thornton Aled Bryant Nick Jones |
format |
Journal article |
container_title |
Clinical & Experimental Immunology |
container_volume |
182 |
container_issue |
1 |
container_start_page |
69 |
publishDate |
2015 |
institution |
Swansea University |
doi_str_mv |
10.1111/cei.12662 |
college_str |
Faculty of Medicine, Health and Life Sciences |
hierarchytype |
|
hierarchy_top_id |
facultyofmedicinehealthandlifesciences |
hierarchy_top_title |
Faculty of Medicine, Health and Life Sciences |
hierarchy_parent_id |
facultyofmedicinehealthandlifesciences |
hierarchy_parent_title |
Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
document_store_str |
1 |
active_str |
0 |
description |
Leukocytes respond rapidly to pathogenic and other insults with responses ranging from cytokine production through to migration and phagocytosis. These are bioenergetically expensive and increased glycolytic flux provides ATP rapidly to support these essential functions. However, much of this work is from animal studies. To better understand the relative role of glycolysis and oxidative phosphorylation in human leukocytes, especially their utility in a translational research setting, we undertook a study of human peripheral blood mononuclear cells (MNCs) bioenergetics. Glycolysis was essential during LPS-mediated IL-1, IL-6, and TNF production as 2-deoxy-D-glucose significantly decreased output of all three cytokines. After optimising cell numbers and the concentrations of all activators and inhibitors, oxidative phosphorylation and glycolysis profiles of fresh and cryopreserved/resuscitated MNCs were determined to explore the utility of MNCs for determining the bioenergetics health profile in multiple clinical settings. While the LPS-induced cytokine response did not differ significantly between fresh and resuscitated cells from the same donors, cryopreservation/resuscitation significantly affected some measures of oxidative phosphorylation mostly, but also glycolysis. Bioenergetics analysis of human MNCs provides a quick, effective means to measure the bioenergetics health index of many individuals but cryopreserved cells are not suitable for such an analysis. The translational utility of this approach was tested by comparing MNCs of pregnant and non-pregnant women to reveal increased bioenergetics health index with pregnancy but significantly reduced basal glycolysis and glycolytic capacity. More detailed analysis of discrete leukocyte populations would be required to understand the relative roles of glycolysis and oxidative phosphorylation during inflammation and other immune responses. |
published_date |
2015-12-31T03:27:44Z |
_version_ |
1763751037033775104 |
score |
11.028886 |