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New methods for analysis of oxysterols and related compounds by LC–MS / William Griffiths, Jonas Abdel-Khalik, Peter J. Crick, Eylan Yutuc, Yuqin Wang

The Journal of Steroid Biochemistry and Molecular Biology, Volume: 162, Pages: 4 - 26

Swansea University Authors: William Griffiths, Eylan Yutuc

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Abstract

Oxysterols are oxygenated forms of cholesterol or its precursors. They are formed enzymatically and via reactive oxygen species. Oxysterols are intermediates in bile acid and steroid hormone biosynthetic pathways and are also bioactive molecules in their own right, being ligands to nuclear receptors...

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Published in: The Journal of Steroid Biochemistry and Molecular Biology
ISSN: 09600760
Published: 2016
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URI: https://cronfa.swan.ac.uk/Record/cronfa25192
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first_indexed 2015-12-22T01:52:40Z
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spelling 2020-05-22T18:50:39.7345877 v2 25192 2015-12-21 New methods for analysis of oxysterols and related compounds by LC–MS 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 99332f073ce913a9b7d8b6441b17516d 0000-0001-9971-1950 Eylan Yutuc Eylan Yutuc true false 2015-12-21 BMS Oxysterols are oxygenated forms of cholesterol or its precursors. They are formed enzymatically and via reactive oxygen species. Oxysterols are intermediates in bile acid and steroid hormone biosynthetic pathways and are also bioactive molecules in their own right, being ligands to nuclear receptors and also regulators of the processing of steroid regulatory element-binding proteins (SREBPs) to their active forms as transcription factors regulating cholesterol and fatty acid biosynthesis. Oxysterols are implicated in the pathogenesis of multiple disease states ranging from atherosclerosis and cancer to multiple sclerosis and other neurodegenerative diseases including Alzheimer’s and Parkinson’s disease. Analysis of oxysterols is challenging on account of their low abundance in biological systems in comparison to cholesterol, and due to the propensity of cholesterol to undergo oxidation in air to generate oxysterols with the same structures as those present endogenously. In this article we review the mass spectrometry-based methods for oxysterol analysis paying particular attention to analysis by liquid chromatography – mass spectrometry (LC-MS). Journal Article The Journal of Steroid Biochemistry and Molecular Biology 162 4 26 09600760 1 9 2016 2016-09-01 10.1016/j.jsbmb.2015.11.017 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University RCUK, BBSRC, BB/I001735/1 2020-05-22T18:50:39.7345877 2015-12-21T09:57:00.5733669 Swansea University Medical School Medicine William Griffiths 0000-0002-4129-6616 1 Jonas Abdel-Khalik 2 Peter J. Crick 3 Eylan Yutuc 0000-0001-9971-1950 4 Yuqin Wang 5 0025192-09012017151047.pdf griffiths.1-s2.0-S0960076015301436-main.pdf 2017-01-09T15:10:47.4970000 Output 2320478 application/pdf Version of Record true 2017-01-09T00:00:00.0000000 Distributed under the terms of a CC-BY license. true
title New methods for analysis of oxysterols and related compounds by LC–MS
spellingShingle New methods for analysis of oxysterols and related compounds by LC–MS
William, Griffiths
Eylan, Yutuc
title_short New methods for analysis of oxysterols and related compounds by LC–MS
title_full New methods for analysis of oxysterols and related compounds by LC–MS
title_fullStr New methods for analysis of oxysterols and related compounds by LC–MS
title_full_unstemmed New methods for analysis of oxysterols and related compounds by LC–MS
title_sort New methods for analysis of oxysterols and related compounds by LC–MS
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
99332f073ce913a9b7d8b6441b17516d
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William, Griffiths
99332f073ce913a9b7d8b6441b17516d_***_Eylan, Yutuc
author William, Griffiths
Eylan, Yutuc
author2 William Griffiths
Jonas Abdel-Khalik
Peter J. Crick
Eylan Yutuc
Yuqin Wang
format Journal article
container_title The Journal of Steroid Biochemistry and Molecular Biology
container_volume 162
container_start_page 4
publishDate 2016
institution Swansea University
issn 09600760
doi_str_mv 10.1016/j.jsbmb.2015.11.017
college_str Swansea University Medical School
hierarchytype
hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 1
active_str 0
description Oxysterols are oxygenated forms of cholesterol or its precursors. They are formed enzymatically and via reactive oxygen species. Oxysterols are intermediates in bile acid and steroid hormone biosynthetic pathways and are also bioactive molecules in their own right, being ligands to nuclear receptors and also regulators of the processing of steroid regulatory element-binding proteins (SREBPs) to their active forms as transcription factors regulating cholesterol and fatty acid biosynthesis. Oxysterols are implicated in the pathogenesis of multiple disease states ranging from atherosclerosis and cancer to multiple sclerosis and other neurodegenerative diseases including Alzheimer’s and Parkinson’s disease. Analysis of oxysterols is challenging on account of their low abundance in biological systems in comparison to cholesterol, and due to the propensity of cholesterol to undergo oxidation in air to generate oxysterols with the same structures as those present endogenously. In this article we review the mass spectrometry-based methods for oxysterol analysis paying particular attention to analysis by liquid chromatography – mass spectrometry (LC-MS).
published_date 2016-09-01T03:39:55Z
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