Journal article 1056 views
Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
Zhaokai Meng,
Sandra C. Bustamante Lopez,
Kenith Meissner,
Vladislav V. Yakovlev
Journal of Biophotonics, Volume: 9, Issue: 3, Pages: 201 - 207
Swansea University Author: Kenith Meissner
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DOI (Published version): 10.1002/jbio.201500163
Abstract
Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it...
| Published in: | Journal of Biophotonics |
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| Published: |
2016
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| Online Access: |
http://onlinelibrary.wiley.com/doi/10.1002/jbio.201500163/abstract |
| URI: | https://cronfa.swan.ac.uk/Record/cronfa27487 |
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| Abstract: |
Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it possible to image scattering samples without substantial degradation of the data quality. However, measurements at the cellular- and subcellular-level have never been performed to date due to the limited signal strength. In this report, by adopting our recently optimized VIPA-based Brillouin spectrometer, we probed the microscopic viscoelasticity of individual red blood cells. These measurements were supplemented by chemically specific measurements using Raman microspectroscopy. |
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| Item Description: |
Published in a leading biomedical optics journal (Impact factor: 4.447), this work represents the first combined Raman/Brillouin study of red blood cell biochemistry/mechanical properties. The power in the combined techniques emerges from the fact that both techniques do not require tagging of the cells. The research from this developing international (Yakovlev, Texas A&M University, USA) collaboration was highlighted in our recently awarded EPSRC Platform grant proposal, “Engineering Blood Diagnostics: Integrated Platforms for Advanced Detection and Analysis.” Results from this work have been presented at international conferences was well as invited seminars at Imperial College and the University of Stuttgart. |
| College: |
Faculty of Science and Engineering |
| Issue: |
3 |
| Start Page: |
201 |
| End Page: |
207 |