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Epitaxial graphene immunosensor for human chorionic gonadotropin

Sofia Teixeira, G. Burwell, A. Castaing, D. Gonzalez, R.S. Conlan, O.J. Guy, Owen Guy Orcid Logo, Steve Conlan Orcid Logo, Deya Gonzalez Orcid Logo, Sofia Rodrigues Teixeira

Sensors and Actuators B: Chemical, Volume: 190, Pages: 723 - 729

Swansea University Authors: Owen Guy Orcid Logo, Steve Conlan Orcid Logo, Deya Gonzalez Orcid Logo, Sofia Rodrigues Teixeira

DOI (Published version): 10.1016/j.snb.2013.09.019

Abstract

Human chorionic gonadotropin (hCG), a 37 kDa glycoprotein hormone, is a key diagnostic marker of pregnancy and has been cited as an important biomarker in relation to cancerous tumors found in the prostate, ovaries and bladder.A novel chemically-modified epitaxial graphene diagnostic sensor has been...

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Published in: Sensors and Actuators B: Chemical
Published: 2014
URI: https://cronfa.swan.ac.uk/Record/cronfa28115
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spelling 2018-01-19T14:23:50.8840025 v2 28115 2016-05-20 Epitaxial graphene immunosensor for human chorionic gonadotropin c7fa5949b8528e048c5b978005f66794 0000-0002-6449-4033 Owen Guy Owen Guy true false 0bb6bd247e32fb4249de62c0013b51cb 0000-0002-2562-3461 Steve Conlan Steve Conlan true false bafdf635eb81280304eedf4b18e65d4e 0000-0002-1838-6752 Deya Gonzalez Deya Gonzalez true false cdbc4e7462ad09634638f5b3d7ecf2d3 Sofia Rodrigues Teixeira Sofia Rodrigues Teixeira true false 2016-05-20 CHEM Human chorionic gonadotropin (hCG), a 37 kDa glycoprotein hormone, is a key diagnostic marker of pregnancy and has been cited as an important biomarker in relation to cancerous tumors found in the prostate, ovaries and bladder.A novel chemically-modified epitaxial graphene diagnostic sensor has been developed for ultrasensitive detection of the biomarker hCG. Multi-layer epitaxial graphene (MEG), grown on silicon carbide substrates, was patterned using electron beam lithography to produce channel based devices. The MEG channels have been amine terminated using 3-Aminopropyl-triethoxysilane (APTES) in order to attach the anti-hCG antibody to the channel.Detection of binding of hCG with its graphene-bound antibody was monitored by measuring reduction of the channel current of the graphene biosensor. The sensitivity of the sensor device was investigated using varying concentrations of hCG, with changes in the channel resistance of the sensor observed upon exposure to hCG. The detection limit of the sensor was 0.62 ng/mL and the sensor showed a linear response to hCG in the range 0.62–5.62 ng/mL with a response of 142 Ω/ng/mL. At concentrations above 5.62 ng/mL the sensor begins to saturate. Journal Article Sensors and Actuators B: Chemical 190 723 729 31 1 2014 2014-01-31 10.1016/j.snb.2013.09.019 COLLEGE NANME Chemistry COLLEGE CODE CHEM Swansea University 2018-01-19T14:23:50.8840025 2016-05-20T11:02:11.6318106 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemistry Sofia Teixeira 1 G. Burwell 2 A. Castaing 3 D. Gonzalez 4 R.S. Conlan 5 O.J. Guy 6 Owen Guy 0000-0002-6449-4033 7 Steve Conlan 0000-0002-2562-3461 8 Deya Gonzalez 0000-0002-1838-6752 9 Sofia Rodrigues Teixeira 10 0028115-18072016153351.pdf Epitaxial_graphene_immunosensorv2.pdf 2016-07-18T15:33:51.2070000 Output 526996 application/pdf Accepted Manuscript true 2016-07-18T00:00:00.0000000 false
title Epitaxial graphene immunosensor for human chorionic gonadotropin
spellingShingle Epitaxial graphene immunosensor for human chorionic gonadotropin
Owen Guy
Steve Conlan
Deya Gonzalez
Sofia Rodrigues Teixeira
title_short Epitaxial graphene immunosensor for human chorionic gonadotropin
title_full Epitaxial graphene immunosensor for human chorionic gonadotropin
title_fullStr Epitaxial graphene immunosensor for human chorionic gonadotropin
title_full_unstemmed Epitaxial graphene immunosensor for human chorionic gonadotropin
title_sort Epitaxial graphene immunosensor for human chorionic gonadotropin
author_id_str_mv c7fa5949b8528e048c5b978005f66794
0bb6bd247e32fb4249de62c0013b51cb
bafdf635eb81280304eedf4b18e65d4e
cdbc4e7462ad09634638f5b3d7ecf2d3
author_id_fullname_str_mv c7fa5949b8528e048c5b978005f66794_***_Owen Guy
0bb6bd247e32fb4249de62c0013b51cb_***_Steve Conlan
bafdf635eb81280304eedf4b18e65d4e_***_Deya Gonzalez
cdbc4e7462ad09634638f5b3d7ecf2d3_***_Sofia Rodrigues Teixeira
author Owen Guy
Steve Conlan
Deya Gonzalez
Sofia Rodrigues Teixeira
author2 Sofia Teixeira
G. Burwell
A. Castaing
D. Gonzalez
R.S. Conlan
O.J. Guy
Owen Guy
Steve Conlan
Deya Gonzalez
Sofia Rodrigues Teixeira
format Journal article
container_title Sensors and Actuators B: Chemical
container_volume 190
container_start_page 723
publishDate 2014
institution Swansea University
doi_str_mv 10.1016/j.snb.2013.09.019
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemistry{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemistry
document_store_str 1
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description Human chorionic gonadotropin (hCG), a 37 kDa glycoprotein hormone, is a key diagnostic marker of pregnancy and has been cited as an important biomarker in relation to cancerous tumors found in the prostate, ovaries and bladder.A novel chemically-modified epitaxial graphene diagnostic sensor has been developed for ultrasensitive detection of the biomarker hCG. Multi-layer epitaxial graphene (MEG), grown on silicon carbide substrates, was patterned using electron beam lithography to produce channel based devices. The MEG channels have been amine terminated using 3-Aminopropyl-triethoxysilane (APTES) in order to attach the anti-hCG antibody to the channel.Detection of binding of hCG with its graphene-bound antibody was monitored by measuring reduction of the channel current of the graphene biosensor. The sensitivity of the sensor device was investigated using varying concentrations of hCG, with changes in the channel resistance of the sensor observed upon exposure to hCG. The detection limit of the sensor was 0.62 ng/mL and the sensor showed a linear response to hCG in the range 0.62–5.62 ng/mL with a response of 142 Ω/ng/mL. At concentrations above 5.62 ng/mL the sensor begins to saturate.
published_date 2014-01-31T03:34:12Z
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