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Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum

Myrto-panagiota Zacharof, Clotilde Vouzelaud, Stephen J. Mandale, Robert Lovitt

Biomass and Bioenergy, Volume: 81, Pages: 294 - 303

Swansea University Authors: Myrto-panagiota Zacharof, Robert Lovitt

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DOI (Published version): 10.1016/j.biombioe.2015.07.018

Abstract

Spent digester streams were reformulated into nutrient rich effluents, capable of supporting biotechnological production of platform chemicals. This was achieved, using a set of physical pre-treatments including, sedimentation, dilution and sieving, followed by cross-flow microfiltration processing...

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Published in: Biomass and Bioenergy
Published: 2015
URI: https://cronfa.swan.ac.uk/Record/cronfa28938
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spelling 2016-06-17T13:53:39.4425553 v2 28938 2016-06-17 Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum 58dbbe00d55ca29ad8b9fb78ba86fe46 Myrto-panagiota Zacharof Myrto-panagiota Zacharof true false 130c3c35f45826bb0f4836305e8e51c7 Robert Lovitt Robert Lovitt true false 2016-06-17 Spent digester streams were reformulated into nutrient rich effluents, capable of supporting biotechnological production of platform chemicals. This was achieved, using a set of physical pre-treatments including, sedimentation, dilution and sieving, followed by cross-flow microfiltration processing to give particle and cell free nutrient streams with a C:N:P molar ratio of 1:1.35:0.03. These streams were inoculated on bench scale, with Clostridium butyricum NCIMB 7432, a well-known acids producer, giving good growth rates (μmax 0.24 h−1) and acids concentrations of 110 mM acetic acid and 18 mM per litre butyric acid. When the treated effluent was fortified with 2% w/v glucose there was a significant improvement with a 0.38 h−1 μmax and platform chemicals concentration of 279 mM acetic acid and 32 mM butyric acid per litre. These media gave comparable performance with the synthetic media, therefore demonstrating a valid alternative solution to commercial media preparations. The cost of treating the excess digestate by microfiltration within the scope of formulation to nutrient media has been calculated at 0.0033 $USD/kg, 3 times lower than the cost of production of nutrient media. Journal Article Biomass and Bioenergy 81 294 303 31 12 2015 2015-12-31 10.1016/j.biombioe.2015.07.018 COLLEGE NANME COLLEGE CODE Swansea University 2016-06-17T13:53:39.4425553 2016-06-17T13:53:39.4425553 College of Engineering College level Myrto-panagiota Zacharof 1 Clotilde Vouzelaud 2 Stephen J. Mandale 3 Robert Lovitt 4
title Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum
spellingShingle Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum
Myrto-panagiota Zacharof
Robert Lovitt
title_short Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum
title_full Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum
title_fullStr Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum
title_full_unstemmed Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum
title_sort Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum
author_id_str_mv 58dbbe00d55ca29ad8b9fb78ba86fe46
130c3c35f45826bb0f4836305e8e51c7
author_id_fullname_str_mv 58dbbe00d55ca29ad8b9fb78ba86fe46_***_Myrto-panagiota Zacharof
130c3c35f45826bb0f4836305e8e51c7_***_Robert Lovitt
author Myrto-panagiota Zacharof
Robert Lovitt
author2 Myrto-panagiota Zacharof
Clotilde Vouzelaud
Stephen J. Mandale
Robert Lovitt
format Journal article
container_title Biomass and Bioenergy
container_volume 81
container_start_page 294
publishDate 2015
institution Swansea University
doi_str_mv 10.1016/j.biombioe.2015.07.018
college_str College of Engineering
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hierarchy_top_id collegeofengineering
hierarchy_top_title College of Engineering
hierarchy_parent_id collegeofengineering
hierarchy_parent_title College of Engineering
department_str College level{{{_:::_}}}College of Engineering{{{_:::_}}}College level
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description Spent digester streams were reformulated into nutrient rich effluents, capable of supporting biotechnological production of platform chemicals. This was achieved, using a set of physical pre-treatments including, sedimentation, dilution and sieving, followed by cross-flow microfiltration processing to give particle and cell free nutrient streams with a C:N:P molar ratio of 1:1.35:0.03. These streams were inoculated on bench scale, with Clostridium butyricum NCIMB 7432, a well-known acids producer, giving good growth rates (μmax 0.24 h−1) and acids concentrations of 110 mM acetic acid and 18 mM per litre butyric acid. When the treated effluent was fortified with 2% w/v glucose there was a significant improvement with a 0.38 h−1 μmax and platform chemicals concentration of 279 mM acetic acid and 32 mM butyric acid per litre. These media gave comparable performance with the synthetic media, therefore demonstrating a valid alternative solution to commercial media preparations. The cost of treating the excess digestate by microfiltration within the scope of formulation to nutrient media has been calculated at 0.0033 $USD/kg, 3 times lower than the cost of production of nutrient media.
published_date 2015-12-31T03:40:18Z
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score 10.877631