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Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues†

Aled Bryant Orcid Logo, Samantha Spencer-Harty, Sian-eleri Owens Orcid Logo, Ruth Jones Orcid Logo, Cathy Thornton Orcid Logo

Biology of Reproduction, Volume: 96, Issue: 3, Pages: 576 - 586

Swansea University Authors: Aled Bryant Orcid Logo, Sian-eleri Owens Orcid Logo, Ruth Jones Orcid Logo, Cathy Thornton Orcid Logo

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DOI (Published version): 10.1095/biolreprod.116.145680

Abstract

Local and systemic inflammation is a key feature of preterm and term labour and delivery. Pro-inflammatory mediators are produced by gestation-associated tissues in response to a broad range of pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs). Interleuki...

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Published in: Biology of Reproduction
ISSN: 0006-3363 1529-7268
Published: Oxford Academic 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa31647
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Pro-inflammatory mediators are produced by gestation-associated tissues in response to a broad range of pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs). Interleukin (IL)-4, IL-10 and IL-13 are well recognised anti-inflammatory cytokines and might have potential as anti-inflammatory therapies to prevent preterm birth. The objective of this study was to determine if IL-4 and IL-13 exert anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated production of pro-inflammatory cytokines produced by human term gestation-associated tissues (placenta, choriodecidua and amnion). Both IL-4 and IL-13 reduced LPS-stimulated IL-1&#x3B2; and macrophage inflammatory protein (MIP)-1&#x3B1; with this effect diminishing with delay to exposure to IL-4 or IL-13; there was no effect on LPS-stimulated prostaglandin production. IL-4 receptor &#x3B1; (IL-4R&#x3B1;) was expressed throughout the placenta, choriodecidua and amnion, and the inhibitory effects of IL-4 and IL-13 were IL-4R&#x3B1;-dependent. A combination of IL-4 and IL-13 did not enhance the anti-inflammatory potential of either cytokine; however, a combination of IL-4 and IL-10 had a greater anti-inflammatory effect than either cytokine alone. These findings demonstrate that human term gestation-associated tissues are responsive to the anti-inflammatory cytokines IL-4 and IL-13, which could down-regulate LPS induced cytokine production in these tissues. 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spelling 2022-02-28T13:00:29.6988010 v2 31647 2017-01-19 Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues† 021f7adc0923f6d2c2e2fc269758b8fe 0000-0002-4650-4672 Aled Bryant Aled Bryant true false 721deb4604d122019244cfdf08820cbe 0000-0003-1806-5235 Sian-eleri Owens Sian-eleri Owens true false a1a281c8720685c422892ef168d4b279 0000-0001-5811-8827 Ruth Jones Ruth Jones true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2017-01-19 PMSC Local and systemic inflammation is a key feature of preterm and term labour and delivery. Pro-inflammatory mediators are produced by gestation-associated tissues in response to a broad range of pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs). Interleukin (IL)-4, IL-10 and IL-13 are well recognised anti-inflammatory cytokines and might have potential as anti-inflammatory therapies to prevent preterm birth. The objective of this study was to determine if IL-4 and IL-13 exert anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated production of pro-inflammatory cytokines produced by human term gestation-associated tissues (placenta, choriodecidua and amnion). Both IL-4 and IL-13 reduced LPS-stimulated IL-1β and macrophage inflammatory protein (MIP)-1α with this effect diminishing with delay to exposure to IL-4 or IL-13; there was no effect on LPS-stimulated prostaglandin production. IL-4 receptor α (IL-4Rα) was expressed throughout the placenta, choriodecidua and amnion, and the inhibitory effects of IL-4 and IL-13 were IL-4Rα-dependent. A combination of IL-4 and IL-13 did not enhance the anti-inflammatory potential of either cytokine; however, a combination of IL-4 and IL-10 had a greater anti-inflammatory effect than either cytokine alone. These findings demonstrate that human term gestation-associated tissues are responsive to the anti-inflammatory cytokines IL-4 and IL-13, which could down-regulate LPS induced cytokine production in these tissues. Anti-inflammatory cytokines might offer an adjunct to existing therapeutics to prevent adverse obstetric outcome. Journal Article Biology of Reproduction 96 3 576 586 Oxford Academic 0006-3363 1529-7268 inflammation, pregnancy, placenta, interleukin-4, interleukin-13 31 3 2017 2017-03-31 10.1095/biolreprod.116.145680 COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University 2022-02-28T13:00:29.6988010 2017-01-19T09:56:26.4805074 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Aled Bryant 0000-0002-4650-4672 1 Samantha Spencer-Harty 2 Sian-eleri Owens 0000-0003-1806-5235 3 Ruth Jones 0000-0001-5811-8827 4 Cathy Thornton 0000-0002-5153-573X 5 0031647-20022017155623.pdf IL4andIL13PlacentaCronfa.pdf 2017-02-20T15:56:23.4930000 Output 549170 application/pdf Accepted Manuscript true 2018-01-27T00:00:00.0000000 This is a pre-copyedited, author-produced version of an article accepted for publication in Biology of Reproduction following peer review. The version of record is available online at: 10.1095/biolreprod.116.145680 true eng
title Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues†
spellingShingle Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues†
Aled Bryant
Sian-eleri Owens
Ruth Jones
Cathy Thornton
title_short Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues†
title_full Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues†
title_fullStr Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues†
title_full_unstemmed Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues†
title_sort Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues†
author_id_str_mv 021f7adc0923f6d2c2e2fc269758b8fe
721deb4604d122019244cfdf08820cbe
a1a281c8720685c422892ef168d4b279
c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv 021f7adc0923f6d2c2e2fc269758b8fe_***_Aled Bryant
721deb4604d122019244cfdf08820cbe_***_Sian-eleri Owens
a1a281c8720685c422892ef168d4b279_***_Ruth Jones
c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
author Aled Bryant
Sian-eleri Owens
Ruth Jones
Cathy Thornton
author2 Aled Bryant
Samantha Spencer-Harty
Sian-eleri Owens
Ruth Jones
Cathy Thornton
format Journal article
container_title Biology of Reproduction
container_volume 96
container_issue 3
container_start_page 576
publishDate 2017
institution Swansea University
issn 0006-3363
1529-7268
doi_str_mv 10.1095/biolreprod.116.145680
publisher Oxford Academic
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description Local and systemic inflammation is a key feature of preterm and term labour and delivery. Pro-inflammatory mediators are produced by gestation-associated tissues in response to a broad range of pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs). Interleukin (IL)-4, IL-10 and IL-13 are well recognised anti-inflammatory cytokines and might have potential as anti-inflammatory therapies to prevent preterm birth. The objective of this study was to determine if IL-4 and IL-13 exert anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated production of pro-inflammatory cytokines produced by human term gestation-associated tissues (placenta, choriodecidua and amnion). Both IL-4 and IL-13 reduced LPS-stimulated IL-1β and macrophage inflammatory protein (MIP)-1α with this effect diminishing with delay to exposure to IL-4 or IL-13; there was no effect on LPS-stimulated prostaglandin production. IL-4 receptor α (IL-4Rα) was expressed throughout the placenta, choriodecidua and amnion, and the inhibitory effects of IL-4 and IL-13 were IL-4Rα-dependent. A combination of IL-4 and IL-13 did not enhance the anti-inflammatory potential of either cytokine; however, a combination of IL-4 and IL-10 had a greater anti-inflammatory effect than either cytokine alone. These findings demonstrate that human term gestation-associated tissues are responsive to the anti-inflammatory cytokines IL-4 and IL-13, which could down-regulate LPS induced cytokine production in these tissues. Anti-inflammatory cytokines might offer an adjunct to existing therapeutics to prevent adverse obstetric outcome.
published_date 2017-03-31T03:38:40Z
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