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Characterisation of a divergent progenitor cell sub-populations in human osteoarthritic cartilage: the role of telomere erosion and replicative senescence / Christopher R. Fellows; Rebecca Williams; Iwan R. Davies; Kajal Gohil; Duncan M. Baird; John Fairclough; Paul Rooney; Charles W. Archer; Ilyas M. Khan

Scientific Reports, Volume: 7, Start page: 41421

Swansea University Author: Khan, Ilyas

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DOI (Published version): 10.1038/srep41421

Abstract

In recent years it has become increasingly clear that articular cartilage harbours a viable pool ofprogenitor cells and interest has focussed on their role during development and disease. Analysis ofprogenitor numbers using fluorescence-activated sorting techniques has resulted in wide-rangingestima...

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Published in: Scientific Reports
ISSN: 2045-2322
Published: 2017
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa31768
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Abstract: In recent years it has become increasingly clear that articular cartilage harbours a viable pool ofprogenitor cells and interest has focussed on their role during development and disease. Analysis ofprogenitor numbers using fluorescence-activated sorting techniques has resulted in wide-rangingestimates, which may be the result of context-dependent expression of cell surface markers. Wehave used a colony-forming assay to reliably determine chondroprogenitor numbers in normal andosteoarthritic cartilage where we observed a 2-fold increase in diseased tissue (P < 0.0001). Intriguingly,cell kinetic analysis of clonal isolates derived from single and multiple donors of osteoarthritic cartilagerevealed the presence of a divergent progenitor subpopulation characterised by an early senescentphenotype. Divergent sub-populations displayed increased senescence-associated β–galactosidaseactivity, lower average telomere lengths but retained the capacity to undergo multi-lineagedifferentiation. Osteoarthritis is an age-related disease and cellular senescence is predicted to be asignificant component of the pathological process. This study shows that although early senescenceis an inherent property of a subset of activated progenitors, there is also a pool of progenitors withextended viability and regenerative potential residing within osteoarthritic cartilage.
Keywords: osteoarthritis cartilage chondroprogenitor telomere senescence
College: Swansea University Medical School
Start Page: 41421