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Production and regulation of interleukin-1 family cytokines at the materno-fetal interface / Louis M. Scott; Aled Bryant; April Rees; Billy Down; Ruth Jones; Catherine Thornton

Cytokine, Volume: 99, Pages: 194 - 202

Swansea University Authors: Aled, Bryant, Ruth, Jones, Catherine, Thornton

Abstract

IL-1 family members regulate innate immune responses, are produced by gestation-associated tissues, and have a role in healthy and adverse pregnancy outcomes. To better understand their role at the materno-fetal interface we used a human tissue explant model to map lipopolysaccharide (LPS)-stimulate...

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Published in: Cytokine
ISSN: 10434666
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa34588
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spelling 2020-05-28T15:21:00.0381212 v2 34588 2017-07-10 Production and regulation of interleukin-1 family cytokines at the materno-fetal interface 021f7adc0923f6d2c2e2fc269758b8fe 0000-0002-4650-4672 Aled Bryant Aled Bryant true false a1a281c8720685c422892ef168d4b279 0000-0001-5811-8827 Ruth Jones Ruth Jones true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Catherine Thornton Catherine Thornton true false 2017-07-10 PMSC IL-1 family members regulate innate immune responses, are produced by gestation-associated tissues, and have a role in healthy and adverse pregnancy outcomes. To better understand their role at the materno-fetal interface we used a human tissue explant model to map lipopolysaccharide (LPS)-stimulated production of IL-1α, IL-1β, IL-18, IL-33, IL-1Ra, IL-18BPa, ST2 and IL-1RAcP by placenta, choriodecidua and amnion. Caspase-dependent processing of IL-1α, IL-1β, IL-18, and IL-33 and the ability of IL-1α, IL-1β, IL-18, and IL-33 to regulate the production of IL-1RA, IL-18BPa, ST2 and IL-1RAcP was also determined. LPS acted as a potent inducer of IL-1 family member expression especially in the placenta and choriodecidua with the response by the amnion restricted to IL-1β. Caspases-1, 4 and 8 contributed to LPS-stimulated production of IL-1 and IL-18, whereas calpain was required for IL-1 production. Exogenous administration of IL-1α, IL-1β, IL-18, and IL-33 lead to differential expression of IL-1Ra, IL-18BPa, ST2 and IL-1RAcP across all tissues examined. Most notable were the counter-regulatory effect of LPS on IL-1 and IL-1Ra in the amnion and the broad responsiveness of the amnion to IL-1 family cytokines for increased production of immunomodulatory peptides and soluble receptors. The placenta and membranes vary not only in their output of various IL-1 family members but also in their counter-regulatory mechanisms through endogenous inhibitory peptides, processing enzymes and soluble decoy receptors. This interactive network of inflammatory mediators likely contributes to innate defence mechanisms at the materno-fetal interface to limit, in particular, the detrimental effects of microbial invasion. Journal Article Cytokine 99 194 202 10434666 Cytokines, IL-1 family, Placenta, Preterm labour, Reproductive Immunology. 1 11 2017 2017-11-01 10.1016/j.cyto.2017.07.005 COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University 2020-05-28T15:21:00.0381212 2017-07-10T11:39:33.3564507 Swansea University Medical School Medicine Louis M. Scott 1 Aled Bryant 0000-0002-4650-4672 2 April Rees 3 Billy Down 4 Ruth Jones 0000-0001-5811-8827 5 Catherine Thornton 0000-0002-5153-573X 6 0034588-02082017152533.pdf 34588.pdf 2017-08-02T15:25:33.3630000 Output 836451 application/pdf Accepted Manuscript true 2018-07-18T00:00:00.0000000 true eng
title Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
spellingShingle Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
Aled, Bryant
Ruth, Jones
Catherine, Thornton
title_short Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
title_full Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
title_fullStr Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
title_full_unstemmed Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
title_sort Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
author_id_str_mv 021f7adc0923f6d2c2e2fc269758b8fe
a1a281c8720685c422892ef168d4b279
c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv 021f7adc0923f6d2c2e2fc269758b8fe_***_Aled, Bryant
a1a281c8720685c422892ef168d4b279_***_Ruth, Jones
c71a7a4be7361094d046d312202bce0c_***_Catherine, Thornton
author Aled, Bryant
Ruth, Jones
Catherine, Thornton
author2 Louis M. Scott
Aled Bryant
April Rees
Billy Down
Ruth Jones
Catherine Thornton
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container_title Cytokine
container_volume 99
container_start_page 194
publishDate 2017
institution Swansea University
issn 10434666
doi_str_mv 10.1016/j.cyto.2017.07.005
college_str Swansea University Medical School
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hierarchy_top_title Swansea University Medical School
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hierarchy_parent_title Swansea University Medical School
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description IL-1 family members regulate innate immune responses, are produced by gestation-associated tissues, and have a role in healthy and adverse pregnancy outcomes. To better understand their role at the materno-fetal interface we used a human tissue explant model to map lipopolysaccharide (LPS)-stimulated production of IL-1α, IL-1β, IL-18, IL-33, IL-1Ra, IL-18BPa, ST2 and IL-1RAcP by placenta, choriodecidua and amnion. Caspase-dependent processing of IL-1α, IL-1β, IL-18, and IL-33 and the ability of IL-1α, IL-1β, IL-18, and IL-33 to regulate the production of IL-1RA, IL-18BPa, ST2 and IL-1RAcP was also determined. LPS acted as a potent inducer of IL-1 family member expression especially in the placenta and choriodecidua with the response by the amnion restricted to IL-1β. Caspases-1, 4 and 8 contributed to LPS-stimulated production of IL-1 and IL-18, whereas calpain was required for IL-1 production. Exogenous administration of IL-1α, IL-1β, IL-18, and IL-33 lead to differential expression of IL-1Ra, IL-18BPa, ST2 and IL-1RAcP across all tissues examined. Most notable were the counter-regulatory effect of LPS on IL-1 and IL-1Ra in the amnion and the broad responsiveness of the amnion to IL-1 family cytokines for increased production of immunomodulatory peptides and soluble receptors. The placenta and membranes vary not only in their output of various IL-1 family members but also in their counter-regulatory mechanisms through endogenous inhibitory peptides, processing enzymes and soluble decoy receptors. This interactive network of inflammatory mediators likely contributes to innate defence mechanisms at the materno-fetal interface to limit, in particular, the detrimental effects of microbial invasion.
published_date 2017-11-01T03:53:37Z
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