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Production and regulation of interleukin-1 family cytokines at the materno-fetal interface

April Rees Orcid Logo, Louis M. Scott, Aled Bryant Orcid Logo, April Rees, Billy Down, Ruth Jones Orcid Logo, Cathy Thornton Orcid Logo

Cytokine, Volume: 99, Pages: 194 - 202

Swansea University Authors: April Rees Orcid Logo, Aled Bryant Orcid Logo, Ruth Jones Orcid Logo, Cathy Thornton Orcid Logo

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DOI (Published version): 10.1016/j.cyto.2017.07.005

Abstract

IL-1 family members regulate innate immune responses, are produced by gestation-associated tissues, and have a role in healthy and adverse pregnancy outcomes. To better understand their role at the materno-fetal interface we used a human tissue explant model to map lipopolysaccharide (LPS)-stimulate...

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Published in: Cytokine
ISSN: 10434666
Published: 2017
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa34588
first_indexed 2017-07-11T12:06:29Z
last_indexed 2020-05-28T18:46:54Z
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To better understand their role at the materno-fetal interface we used a human tissue explant model to map lipopolysaccharide (LPS)-stimulated production of IL-1&#x3B1;, IL-1&#x3B2;, IL-18, IL-33, IL-1Ra, IL-18BPa, ST2 and IL-1RAcP by placenta, choriodecidua and amnion. Caspase-dependent processing of IL-1&#x3B1;, IL-1&#x3B2;, IL-18, and IL-33 and the ability of IL-1&#x3B1;, IL-1&#x3B2;, IL-18, and IL-33 to regulate the production of IL-1RA, IL-18BPa, ST2 and IL-1RAcP was also determined. LPS acted as a potent inducer of IL-1 family member expression especially in the placenta and choriodecidua with the response by the amnion restricted to IL-1&#x3B2;. Caspases-1, 4 and 8 contributed to LPS-stimulated production of IL-1&#xF062; and IL-18, whereas calpain was required for IL-1&#xF061; production. Exogenous administration of IL-1&#x3B1;, IL-1&#x3B2;, IL-18, and IL-33 lead to differential expression of IL-1Ra, IL-18BPa, ST2 and IL-1RAcP across all tissues examined. Most notable were the counter-regulatory effect of LPS on IL-1&#xF062; and IL-1Ra in the amnion and the broad responsiveness of the amnion to IL-1 family cytokines for increased production of immunomodulatory peptides and soluble receptors. The placenta and membranes vary not only in their output of various IL-1 family members but also in their counter-regulatory mechanisms through endogenous inhibitory peptides, processing enzymes and soluble decoy receptors. This interactive network of inflammatory mediators likely contributes to innate defence mechanisms at the materno-fetal interface to limit, in particular, the detrimental effects of microbial invasion.</abstract><type>Journal Article</type><journal>Cytokine</journal><volume>99</volume><paginationStart>194</paginationStart><paginationEnd>202</paginationEnd><publisher/><issnPrint>10434666</issnPrint><keywords>Cytokines, IL-1 family, Placenta, Preterm labour, Reproductive Immunology.</keywords><publishedDay>1</publishedDay><publishedMonth>11</publishedMonth><publishedYear>2017</publishedYear><publishedDate>2017-11-01</publishedDate><doi>10.1016/j.cyto.2017.07.005</doi><url/><notes/><college>COLLEGE NANME</college><department>Medical School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MEDS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2020-05-28T15:21:00.0381212</lastEdited><Created>2017-07-10T11:39:33.3564507</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>April</firstname><surname>Rees</surname><orcid>0000-0002-4408-634X</orcid><order>1</order></author><author><firstname>Louis M.</firstname><surname>Scott</surname><order>2</order></author><author><firstname>Aled</firstname><surname>Bryant</surname><orcid>0000-0002-4650-4672</orcid><order>3</order></author><author><firstname>April</firstname><surname>Rees</surname><order>4</order></author><author><firstname>Billy</firstname><surname>Down</surname><order>5</order></author><author><firstname>Ruth</firstname><surname>Jones</surname><orcid>0000-0001-5811-8827</orcid><order>6</order></author><author><firstname>Cathy</firstname><surname>Thornton</surname><orcid>0000-0002-5153-573X</orcid><order>7</order></author></authors><documents><document><filename>0034588-02082017152533.pdf</filename><originalFilename>34588.pdf</originalFilename><uploaded>2017-08-02T15:25:33.3630000</uploaded><type>Output</type><contentLength>836451</contentLength><contentType>application/pdf</contentType><version>Accepted Manuscript</version><cronfaStatus>true</cronfaStatus><embargoDate>2018-07-18T00:00:00.0000000</embargoDate><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2020-05-28T15:21:00.0381212 v2 34588 2017-07-10 Production and regulation of interleukin-1 family cytokines at the materno-fetal interface ae088f7f8609d2b2ea4666f9b52b3c15 0000-0002-4408-634X April Rees April Rees true false 021f7adc0923f6d2c2e2fc269758b8fe 0000-0002-4650-4672 Aled Bryant Aled Bryant true false a1a281c8720685c422892ef168d4b279 0000-0001-5811-8827 Ruth Jones Ruth Jones true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2017-07-10 MEDS IL-1 family members regulate innate immune responses, are produced by gestation-associated tissues, and have a role in healthy and adverse pregnancy outcomes. To better understand their role at the materno-fetal interface we used a human tissue explant model to map lipopolysaccharide (LPS)-stimulated production of IL-1α, IL-1β, IL-18, IL-33, IL-1Ra, IL-18BPa, ST2 and IL-1RAcP by placenta, choriodecidua and amnion. Caspase-dependent processing of IL-1α, IL-1β, IL-18, and IL-33 and the ability of IL-1α, IL-1β, IL-18, and IL-33 to regulate the production of IL-1RA, IL-18BPa, ST2 and IL-1RAcP was also determined. LPS acted as a potent inducer of IL-1 family member expression especially in the placenta and choriodecidua with the response by the amnion restricted to IL-1β. Caspases-1, 4 and 8 contributed to LPS-stimulated production of IL-1 and IL-18, whereas calpain was required for IL-1 production. Exogenous administration of IL-1α, IL-1β, IL-18, and IL-33 lead to differential expression of IL-1Ra, IL-18BPa, ST2 and IL-1RAcP across all tissues examined. Most notable were the counter-regulatory effect of LPS on IL-1 and IL-1Ra in the amnion and the broad responsiveness of the amnion to IL-1 family cytokines for increased production of immunomodulatory peptides and soluble receptors. The placenta and membranes vary not only in their output of various IL-1 family members but also in their counter-regulatory mechanisms through endogenous inhibitory peptides, processing enzymes and soluble decoy receptors. This interactive network of inflammatory mediators likely contributes to innate defence mechanisms at the materno-fetal interface to limit, in particular, the detrimental effects of microbial invasion. Journal Article Cytokine 99 194 202 10434666 Cytokines, IL-1 family, Placenta, Preterm labour, Reproductive Immunology. 1 11 2017 2017-11-01 10.1016/j.cyto.2017.07.005 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2020-05-28T15:21:00.0381212 2017-07-10T11:39:33.3564507 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine April Rees 0000-0002-4408-634X 1 Louis M. Scott 2 Aled Bryant 0000-0002-4650-4672 3 April Rees 4 Billy Down 5 Ruth Jones 0000-0001-5811-8827 6 Cathy Thornton 0000-0002-5153-573X 7 0034588-02082017152533.pdf 34588.pdf 2017-08-02T15:25:33.3630000 Output 836451 application/pdf Accepted Manuscript true 2018-07-18T00:00:00.0000000 true eng
title Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
spellingShingle Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
April Rees
Aled Bryant
Ruth Jones
Cathy Thornton
title_short Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
title_full Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
title_fullStr Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
title_full_unstemmed Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
title_sort Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
author_id_str_mv ae088f7f8609d2b2ea4666f9b52b3c15
021f7adc0923f6d2c2e2fc269758b8fe
a1a281c8720685c422892ef168d4b279
c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv ae088f7f8609d2b2ea4666f9b52b3c15_***_April Rees
021f7adc0923f6d2c2e2fc269758b8fe_***_Aled Bryant
a1a281c8720685c422892ef168d4b279_***_Ruth Jones
c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
author April Rees
Aled Bryant
Ruth Jones
Cathy Thornton
author2 April Rees
Louis M. Scott
Aled Bryant
April Rees
Billy Down
Ruth Jones
Cathy Thornton
format Journal article
container_title Cytokine
container_volume 99
container_start_page 194
publishDate 2017
institution Swansea University
issn 10434666
doi_str_mv 10.1016/j.cyto.2017.07.005
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description IL-1 family members regulate innate immune responses, are produced by gestation-associated tissues, and have a role in healthy and adverse pregnancy outcomes. To better understand their role at the materno-fetal interface we used a human tissue explant model to map lipopolysaccharide (LPS)-stimulated production of IL-1α, IL-1β, IL-18, IL-33, IL-1Ra, IL-18BPa, ST2 and IL-1RAcP by placenta, choriodecidua and amnion. Caspase-dependent processing of IL-1α, IL-1β, IL-18, and IL-33 and the ability of IL-1α, IL-1β, IL-18, and IL-33 to regulate the production of IL-1RA, IL-18BPa, ST2 and IL-1RAcP was also determined. LPS acted as a potent inducer of IL-1 family member expression especially in the placenta and choriodecidua with the response by the amnion restricted to IL-1β. Caspases-1, 4 and 8 contributed to LPS-stimulated production of IL-1 and IL-18, whereas calpain was required for IL-1 production. Exogenous administration of IL-1α, IL-1β, IL-18, and IL-33 lead to differential expression of IL-1Ra, IL-18BPa, ST2 and IL-1RAcP across all tissues examined. Most notable were the counter-regulatory effect of LPS on IL-1 and IL-1Ra in the amnion and the broad responsiveness of the amnion to IL-1 family cytokines for increased production of immunomodulatory peptides and soluble receptors. The placenta and membranes vary not only in their output of various IL-1 family members but also in their counter-regulatory mechanisms through endogenous inhibitory peptides, processing enzymes and soluble decoy receptors. This interactive network of inflammatory mediators likely contributes to innate defence mechanisms at the materno-fetal interface to limit, in particular, the detrimental effects of microbial invasion.
published_date 2017-11-01T04:21:55Z
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