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Expression and function of NOD-like receptors by human term gestation-associated tissues / Aled Bryant; Ryan J. Bevan; Samantha Spencer-Harty; Louis M. Scott; Ruth H. Jones; Catherine Thornton

Placenta, Volume: 58, Pages: 25 - 32

Swansea University Authors: Aled, Bryant, Catherine, Thornton

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Abstract

Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the mate...

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Published in: Placenta
ISSN: 01434004
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa34833
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spelling 2020-06-01T15:17:38.0725889 v2 34833 2017-07-31 Expression and function of NOD-like receptors by human term gestation-associated tissues 021f7adc0923f6d2c2e2fc269758b8fe 0000-0002-4650-4672 Aled Bryant Aled Bryant true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Catherine Thornton Catherine Thornton true false 2017-07-31 PMSC Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the materno-fetal interface has been postulated. Methods: Gene expression and immunolocalisation of NLR family members in human placenta, choriodecidua, and amnion were examined. Tissue explants were used to examine the response to activators of NOD1 (Tri-DAP), NOD2 (MDP) and NLRP3 (nigericin). Cell/tissue-free supernatants were examined for the production of interleukin (IL)-1, IL-6, IL-8 and IL-10 using specific ELISAs. Results: Expression of transcripts for NOD1, NOD2, NLRP3, NLRC4, NLRX1, NLRP1 and NAIP and protein expression of NOD1, NOD2 and NLRP3 were a broad feature of all term gestation-associated tissues. Production of cytokines was increased significantly in response to all ligands in placenta and choriodecidua, except for MDP-induced IL-10. Similarly, there was a significant increase in cytokine production in the amnion except for MDP induced IL-1β and IL-10 response to either agonist. IL-1β production was dependent on caspase-1 regardless of agonist used or tissue examined.Discussion: Term human gestation-associated tissues express functional NLRs which likely play a role in both sterile and pathogen-driven inflammatory responses at the materno-fetal interface. Journal Article Placenta 58 25 32 01434004 Inflammation; reproductive immunology; preterm labour; NOD-like receptors 31 10 2017 2017-10-31 10.1016/j.placenta.2017.07.017 COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University 2020-06-01T15:17:38.0725889 2017-07-31T11:08:29.4017146 Swansea University Medical School Medicine Aled Bryant 0000-0002-4650-4672 1 Ryan J. Bevan 2 Samantha Spencer-Harty 3 Louis M. Scott 4 Ruth H. Jones 5 Catherine Thornton 0000-0002-5153-573X 6 0034833-15022018143527.pdf NLRexpressionPLACENTAJuly2017RISU.pdf 2018-02-15T14:35:27.5370000 Output 803093 application/pdf Accepted Manuscript true 2018-08-02T00:00:00.0000000 Released under the terms of a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND). true eng
title Expression and function of NOD-like receptors by human term gestation-associated tissues
spellingShingle Expression and function of NOD-like receptors by human term gestation-associated tissues
Aled, Bryant
Catherine, Thornton
title_short Expression and function of NOD-like receptors by human term gestation-associated tissues
title_full Expression and function of NOD-like receptors by human term gestation-associated tissues
title_fullStr Expression and function of NOD-like receptors by human term gestation-associated tissues
title_full_unstemmed Expression and function of NOD-like receptors by human term gestation-associated tissues
title_sort Expression and function of NOD-like receptors by human term gestation-associated tissues
author_id_str_mv 021f7adc0923f6d2c2e2fc269758b8fe
c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv 021f7adc0923f6d2c2e2fc269758b8fe_***_Aled, Bryant
c71a7a4be7361094d046d312202bce0c_***_Catherine, Thornton
author Aled, Bryant
Catherine, Thornton
author2 Aled Bryant
Ryan J. Bevan
Samantha Spencer-Harty
Louis M. Scott
Ruth H. Jones
Catherine Thornton
format Journal article
container_title Placenta
container_volume 58
container_start_page 25
publishDate 2017
institution Swansea University
issn 01434004
doi_str_mv 10.1016/j.placenta.2017.07.017
college_str Swansea University Medical School
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hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 1
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description Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the materno-fetal interface has been postulated. Methods: Gene expression and immunolocalisation of NLR family members in human placenta, choriodecidua, and amnion were examined. Tissue explants were used to examine the response to activators of NOD1 (Tri-DAP), NOD2 (MDP) and NLRP3 (nigericin). Cell/tissue-free supernatants were examined for the production of interleukin (IL)-1, IL-6, IL-8 and IL-10 using specific ELISAs. Results: Expression of transcripts for NOD1, NOD2, NLRP3, NLRC4, NLRX1, NLRP1 and NAIP and protein expression of NOD1, NOD2 and NLRP3 were a broad feature of all term gestation-associated tissues. Production of cytokines was increased significantly in response to all ligands in placenta and choriodecidua, except for MDP-induced IL-10. Similarly, there was a significant increase in cytokine production in the amnion except for MDP induced IL-1β and IL-10 response to either agonist. IL-1β production was dependent on caspase-1 regardless of agonist used or tissue examined.Discussion: Term human gestation-associated tissues express functional NLRs which likely play a role in both sterile and pathogen-driven inflammatory responses at the materno-fetal interface.
published_date 2017-10-31T03:53:31Z
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