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Expression and function of NOD-like receptors by human term gestation-associated tissues
Placenta, Volume: 58, Pages: 25 - 32
Swansea University Authors:
Aled Bryant , Cathy Thornton
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DOI (Published version): 10.1016/j.placenta.2017.07.017
Abstract
Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the mate...
Published in: | Placenta |
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ISSN: | 01434004 |
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2017
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2020-06-01T15:17:38.0725889 v2 34833 2017-07-31 Expression and function of NOD-like receptors by human term gestation-associated tissues 021f7adc0923f6d2c2e2fc269758b8fe 0000-0002-4650-4672 Aled Bryant Aled Bryant true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2017-07-31 PMSC Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the materno-fetal interface has been postulated. Methods: Gene expression and immunolocalisation of NLR family members in human placenta, choriodecidua, and amnion were examined. Tissue explants were used to examine the response to activators of NOD1 (Tri-DAP), NOD2 (MDP) and NLRP3 (nigericin). Cell/tissue-free supernatants were examined for the production of interleukin (IL)-1, IL-6, IL-8 and IL-10 using specific ELISAs. Results: Expression of transcripts for NOD1, NOD2, NLRP3, NLRC4, NLRX1, NLRP1 and NAIP and protein expression of NOD1, NOD2 and NLRP3 were a broad feature of all term gestation-associated tissues. Production of cytokines was increased significantly in response to all ligands in placenta and choriodecidua, except for MDP-induced IL-10. Similarly, there was a significant increase in cytokine production in the amnion except for MDP induced IL-1β and IL-10 response to either agonist. IL-1β production was dependent on caspase-1 regardless of agonist used or tissue examined.Discussion: Term human gestation-associated tissues express functional NLRs which likely play a role in both sterile and pathogen-driven inflammatory responses at the materno-fetal interface. Journal Article Placenta 58 25 32 01434004 Inflammation; reproductive immunology; preterm labour; NOD-like receptors 31 10 2017 2017-10-31 10.1016/j.placenta.2017.07.017 COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University 2020-06-01T15:17:38.0725889 2017-07-31T11:08:29.4017146 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Aled Bryant 0000-0002-4650-4672 1 Ryan J. Bevan 2 Samantha Spencer-Harty 3 Louis M. Scott 4 Ruth H. Jones 5 Cathy Thornton 0000-0002-5153-573X 6 0034833-15022018143527.pdf NLRexpressionPLACENTAJuly2017RISU.pdf 2018-02-15T14:35:27.5370000 Output 803093 application/pdf Accepted Manuscript true 2018-08-02T00:00:00.0000000 Released under the terms of a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND). true eng |
title |
Expression and function of NOD-like receptors by human term gestation-associated tissues |
spellingShingle |
Expression and function of NOD-like receptors by human term gestation-associated tissues Aled Bryant Cathy Thornton |
title_short |
Expression and function of NOD-like receptors by human term gestation-associated tissues |
title_full |
Expression and function of NOD-like receptors by human term gestation-associated tissues |
title_fullStr |
Expression and function of NOD-like receptors by human term gestation-associated tissues |
title_full_unstemmed |
Expression and function of NOD-like receptors by human term gestation-associated tissues |
title_sort |
Expression and function of NOD-like receptors by human term gestation-associated tissues |
author_id_str_mv |
021f7adc0923f6d2c2e2fc269758b8fe c71a7a4be7361094d046d312202bce0c |
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021f7adc0923f6d2c2e2fc269758b8fe_***_Aled Bryant c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton |
author |
Aled Bryant Cathy Thornton |
author2 |
Aled Bryant Ryan J. Bevan Samantha Spencer-Harty Louis M. Scott Ruth H. Jones Cathy Thornton |
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Journal article |
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Placenta |
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58 |
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2017 |
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Swansea University |
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01434004 |
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10.1016/j.placenta.2017.07.017 |
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Faculty of Medicine, Health and Life Sciences |
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Faculty of Medicine, Health and Life Sciences |
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Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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description |
Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the materno-fetal interface has been postulated. Methods: Gene expression and immunolocalisation of NLR family members in human placenta, choriodecidua, and amnion were examined. Tissue explants were used to examine the response to activators of NOD1 (Tri-DAP), NOD2 (MDP) and NLRP3 (nigericin). Cell/tissue-free supernatants were examined for the production of interleukin (IL)-1, IL-6, IL-8 and IL-10 using specific ELISAs. Results: Expression of transcripts for NOD1, NOD2, NLRP3, NLRC4, NLRX1, NLRP1 and NAIP and protein expression of NOD1, NOD2 and NLRP3 were a broad feature of all term gestation-associated tissues. Production of cytokines was increased significantly in response to all ligands in placenta and choriodecidua, except for MDP-induced IL-10. Similarly, there was a significant increase in cytokine production in the amnion except for MDP induced IL-1β and IL-10 response to either agonist. IL-1β production was dependent on caspase-1 regardless of agonist used or tissue examined.Discussion: Term human gestation-associated tissues express functional NLRs which likely play a role in both sterile and pathogen-driven inflammatory responses at the materno-fetal interface. |
published_date |
2017-10-31T03:43:14Z |
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1763752011882299392 |
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11.021648 |