Expression and function of NOD-like receptors by human term gestation-associated tissues

Bryant, Aled; Bevan, Ryan J.; Spencer-Harty, Samantha; Scott, Louis M.; Jones, Ruth H.; Thornton, Cathy

doi:10.1016/j.placenta.2017.07.017

Journal article 1528 views 384 downloads

Expression and function of NOD-like receptors by human term gestation-associated tissues

Aled Bryant

, Ryan J. Bevan, Samantha Spencer-Harty, Louis M. Scott, Ruth H. Jones, Cathy Thornton

Placenta, Volume: 58, Pages: 25 - 32

Swansea University Authors: Aled Bryant , Cathy Thornton

PDF | Accepted Manuscript

Released under the terms of a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND).
Download (811.55KB)

Check full text

DOI (Published version): 10.1016/j.placenta.2017.07.017

Abstract

Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the mate...

Full description

Published in:	Placenta
ISSN:	01434004
Published:	2017
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa34833

first_indexed	2017-07-31T14:43:08Z
last_indexed	2020-06-01T18:46:43Z
id	cronfa34833
recordtype	SURis
fullrecord	<?xml version="1.0"?><rfc1807><datestamp>2020-06-01T15:17:38.0725889</datestamp><bib-version>v2</bib-version><id>34833</id><entry>2017-07-31</entry><title>Expression and function of NOD-like receptors by human term gestation-associated tissues</title><swanseaauthors><author><sid>021f7adc0923f6d2c2e2fc269758b8fe</sid><ORCID>0000-0002-4650-4672</ORCID><firstname>Aled</firstname><surname>Bryant</surname><name>Aled Bryant</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>c71a7a4be7361094d046d312202bce0c</sid><ORCID>0000-0002-5153-573X</ORCID><firstname>Cathy</firstname><surname>Thornton</surname><name>Cathy Thornton</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2017-07-31</date><abstract>Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the materno-fetal interface has been postulated. Methods: Gene expression and immunolocalisation of NLR family members in human placenta, choriodecidua, and amnion were examined. Tissue explants were used to examine the response to activators of NOD1 (Tri-DAP), NOD2 (MDP) and NLRP3 (nigericin). Cell/tissue-free supernatants were examined for the production of interleukin (IL)-1, IL-6, IL-8 and IL-10 using specific ELISAs. Results: Expression of transcripts for NOD1, NOD2, NLRP3, NLRC4, NLRX1, NLRP1 and NAIP and protein expression of NOD1, NOD2 and NLRP3 were a broad feature of all term gestation-associated tissues. Production of cytokines was increased significantly in response to all ligands in placenta and choriodecidua, except for MDP-induced IL-10. Similarly, there was a significant increase in cytokine production in the amnion except for MDP induced IL-1β and IL-10 response to either agonist. IL-1β production was dependent on caspase-1 regardless of agonist used or tissue examined.Discussion: Term human gestation-associated tissues express functional NLRs which likely play a role in both sterile and pathogen-driven inflammatory responses at the materno-fetal interface.</abstract><type>Journal Article</type><journal>Placenta</journal><volume>58</volume><paginationStart>25</paginationStart><paginationEnd>32</paginationEnd><publisher/><issnPrint>01434004</issnPrint><keywords>Inflammation; reproductive immunology; preterm labour; NOD-like receptors</keywords><publishedDay>31</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2017</publishedYear><publishedDate>2017-10-31</publishedDate><doi>10.1016/j.placenta.2017.07.017</doi><url/><notes/><college>COLLEGE NANME</college><CollegeCode>COLLEGE CODE</CollegeCode><institution>Swansea University</institution><apcterm/><lastEdited>2020-06-01T15:17:38.0725889</lastEdited><Created>2017-07-31T11:08:29.4017146</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Aled</firstname><surname>Bryant</surname><orcid>0000-0002-4650-4672</orcid><order>1</order></author><author><firstname>Ryan J.</firstname><surname>Bevan</surname><order>2</order></author><author><firstname>Samantha</firstname><surname>Spencer-Harty</surname><order>3</order></author><author><firstname>Louis M.</firstname><surname>Scott</surname><order>4</order></author><author><firstname>Ruth H.</firstname><surname>Jones</surname><order>5</order></author><author><firstname>Cathy</firstname><surname>Thornton</surname><orcid>0000-0002-5153-573X</orcid><order>6</order></author></authors><documents><document><filename>0034833-15022018143527.pdf</filename><originalFilename>NLRexpressionPLACENTAJuly2017RISU.pdf</originalFilename><uploaded>2018-02-15T14:35:27.5370000</uploaded><type>Output</type><contentLength>803093</contentLength><contentType>application/pdf</contentType><version>Accepted Manuscript</version><cronfaStatus>true</cronfaStatus><embargoDate>2018-08-02T00:00:00.0000000</embargoDate><documentNotes>Released under the terms of a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling	2020-06-01T15:17:38.0725889 v2 34833 2017-07-31 Expression and function of NOD-like receptors by human term gestation-associated tissues 021f7adc0923f6d2c2e2fc269758b8fe 0000-0002-4650-4672 Aled Bryant Aled Bryant true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2017-07-31 Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the materno-fetal interface has been postulated. Methods: Gene expression and immunolocalisation of NLR family members in human placenta, choriodecidua, and amnion were examined. Tissue explants were used to examine the response to activators of NOD1 (Tri-DAP), NOD2 (MDP) and NLRP3 (nigericin). Cell/tissue-free supernatants were examined for the production of interleukin (IL)-1, IL-6, IL-8 and IL-10 using specific ELISAs. Results: Expression of transcripts for NOD1, NOD2, NLRP3, NLRC4, NLRX1, NLRP1 and NAIP and protein expression of NOD1, NOD2 and NLRP3 were a broad feature of all term gestation-associated tissues. Production of cytokines was increased significantly in response to all ligands in placenta and choriodecidua, except for MDP-induced IL-10. Similarly, there was a significant increase in cytokine production in the amnion except for MDP induced IL-1β and IL-10 response to either agonist. IL-1β production was dependent on caspase-1 regardless of agonist used or tissue examined.Discussion: Term human gestation-associated tissues express functional NLRs which likely play a role in both sterile and pathogen-driven inflammatory responses at the materno-fetal interface. Journal Article Placenta 58 25 32 01434004 Inflammation; reproductive immunology; preterm labour; NOD-like receptors 31 10 2017 2017-10-31 10.1016/j.placenta.2017.07.017 COLLEGE NANME COLLEGE CODE Swansea University 2020-06-01T15:17:38.0725889 2017-07-31T11:08:29.4017146 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Aled Bryant 0000-0002-4650-4672 1 Ryan J. Bevan 2 Samantha Spencer-Harty 3 Louis M. Scott 4 Ruth H. Jones 5 Cathy Thornton 0000-0002-5153-573X 6 0034833-15022018143527.pdf NLRexpressionPLACENTAJuly2017RISU.pdf 2018-02-15T14:35:27.5370000 Output 803093 application/pdf Accepted Manuscript true 2018-08-02T00:00:00.0000000 Released under the terms of a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND). true eng
title	Expression and function of NOD-like receptors by human term gestation-associated tissues
spellingShingle	Expression and function of NOD-like receptors by human term gestation-associated tissues Aled Bryant Cathy Thornton
title_short	Expression and function of NOD-like receptors by human term gestation-associated tissues
title_full	Expression and function of NOD-like receptors by human term gestation-associated tissues
title_fullStr	Expression and function of NOD-like receptors by human term gestation-associated tissues
title_full_unstemmed	Expression and function of NOD-like receptors by human term gestation-associated tissues
title_sort	Expression and function of NOD-like receptors by human term gestation-associated tissues
author_id_str_mv	021f7adc0923f6d2c2e2fc269758b8fe c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv	021f7adc0923f6d2c2e2fc269758b8fe_*_Aled Bryant c71a7a4be7361094d046d312202bce0c_*_Cathy Thornton
author	Aled Bryant Cathy Thornton
author2	Aled Bryant Ryan J. Bevan Samantha Spencer-Harty Louis M. Scott Ruth H. Jones Cathy Thornton
format	Journal article
container_title	Placenta
container_volume	58
container_start_page	25
publishDate	2017
institution	Swansea University
issn	01434004
doi_str_mv	10.1016/j.placenta.2017.07.017
college_str	Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str	1
active_str	0
description	Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the materno-fetal interface has been postulated. Methods: Gene expression and immunolocalisation of NLR family members in human placenta, choriodecidua, and amnion were examined. Tissue explants were used to examine the response to activators of NOD1 (Tri-DAP), NOD2 (MDP) and NLRP3 (nigericin). Cell/tissue-free supernatants were examined for the production of interleukin (IL)-1, IL-6, IL-8 and IL-10 using specific ELISAs. Results: Expression of transcripts for NOD1, NOD2, NLRP3, NLRC4, NLRX1, NLRP1 and NAIP and protein expression of NOD1, NOD2 and NLRP3 were a broad feature of all term gestation-associated tissues. Production of cytokines was increased significantly in response to all ligands in placenta and choriodecidua, except for MDP-induced IL-10. Similarly, there was a significant increase in cytokine production in the amnion except for MDP induced IL-1β and IL-10 response to either agonist. IL-1β production was dependent on caspase-1 regardless of agonist used or tissue examined.Discussion: Term human gestation-associated tissues express functional NLRs which likely play a role in both sterile and pathogen-driven inflammatory responses at the materno-fetal interface.
published_date	2017-10-31T07:13:36Z
_version_	1822022871374888960
score	11.085372

Expression and function of NOD-like receptors by human term gestation-associated tissues

Similar Items