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Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus

Luisa C. Carneiro, Carmen Bedford, Sarah Jacca, Alfonso Rosamilia, Vera F. de Lima, Gaetano Donofrio, I. Martin Sheldon, James Cronin Orcid Logo, Martin Sheldon Orcid Logo

Frontiers in Immunology, Volume: 8

Swansea University Authors: James Cronin Orcid Logo, Martin Sheldon Orcid Logo

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DOI (Published version): 10.3389/fimmu.2017.00996

Abstract

Bovine herpesvirus-4 (BoHV-4) and bovine viral diarrhea virus (BVDV) infect the uterus of cattle, often resulting in reduced fertility, or abortion of the fetus, respectively. Here, exposure of primary bovine endometrial cells to BoHV-4 or BVDV modulated the production of inflammatory mediators. Vir...

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Published in: Frontiers in Immunology
ISSN: 1664-3224
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa34992
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spelling 2020-06-22T13:43:22.4520835 v2 34992 2017-08-23 Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus 9cfd17551c0d1f7438895121e4fbb6e8 0000-0002-0590-9462 James Cronin James Cronin true false ab0f74b794e59cc270c69e63ee1d9748 0000-0001-7902-5558 Martin Sheldon Martin Sheldon true false 2017-08-23 BMS Bovine herpesvirus-4 (BoHV-4) and bovine viral diarrhea virus (BVDV) infect the uterus of cattle, often resulting in reduced fertility, or abortion of the fetus, respectively. Here, exposure of primary bovine endometrial cells to BoHV-4 or BVDV modulated the production of inflammatory mediators. Viral pathogen-associated molecular patterns (PAMPs) are detected via pattern-recognition receptors (PRRs). However, the relative contribution of specific PRRs to innate immunity, during viral infection of the uterus, is unclear. Endometrial epithelial and stromal cells constitutively express the PRR Toll-like receptor (TLR)-3, but, the status of retinoic acid-inducible gene I (RIG-I), a sensor of cytosolic nucleic acids, is unknown. Primary endometrial epithelial and stromal cells had low expression of RIG-I, which was increased in stromal cells after 12 h transfection with the TLR3 ligand Poly(I:C), a synthetic analog of double-stranded RNA. Furthermore, short interfering RNA targeting TLR3, or interferon (IFN) regulatory transcription factor 3, an inducer of type I IFN transcription, reduced Poly(I:C)-induced RIG-I protein expression and reduced inflammatory mediator secretion from stromal cells. We conclude that antiviral defense of endometrial stromal cells requires coordinated recognition of PAMPs, initially via TLR3 and later via inducible RIG-I. Journal Article Frontiers in Immunology 8 1664-3224 23 8 2017 2017-08-23 10.3389/fimmu.2017.00996 http://journal.frontiersin.org/article/10.3389/fimmu.2017.00996/full COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University RCUK, BBSRC, BB/1017240/1 2020-06-22T13:43:22.4520835 2017-08-23T13:47:31.2408571 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Luisa C. Carneiro 1 Carmen Bedford 2 Sarah Jacca 3 Alfonso Rosamilia 4 Vera F. de Lima 5 Gaetano Donofrio 6 I. Martin Sheldon 7 James Cronin 0000-0002-0590-9462 8 Martin Sheldon 0000-0001-7902-5558 9 0034992-23082017135224.pdf Carneiro2017.pdf 2017-08-23T13:52:24.6500000 Output 2077782 application/pdf Version of Record true 2017-08-23T00:00:00.0000000 This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). true eng
title Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus
spellingShingle Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus
James Cronin
Martin Sheldon
title_short Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus
title_full Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus
title_fullStr Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus
title_full_unstemmed Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus
title_sort Coordinated Role of Toll-Like Receptor-3 and Retinoic Acid-Inducible Gene-I in the Innate Response of Bovine Endometrial Cells to Virus
author_id_str_mv 9cfd17551c0d1f7438895121e4fbb6e8
ab0f74b794e59cc270c69e63ee1d9748
author_id_fullname_str_mv 9cfd17551c0d1f7438895121e4fbb6e8_***_James Cronin
ab0f74b794e59cc270c69e63ee1d9748_***_Martin Sheldon
author James Cronin
Martin Sheldon
author2 Luisa C. Carneiro
Carmen Bedford
Sarah Jacca
Alfonso Rosamilia
Vera F. de Lima
Gaetano Donofrio
I. Martin Sheldon
James Cronin
Martin Sheldon
format Journal article
container_title Frontiers in Immunology
container_volume 8
publishDate 2017
institution Swansea University
issn 1664-3224
doi_str_mv 10.3389/fimmu.2017.00996
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://journal.frontiersin.org/article/10.3389/fimmu.2017.00996/full
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description Bovine herpesvirus-4 (BoHV-4) and bovine viral diarrhea virus (BVDV) infect the uterus of cattle, often resulting in reduced fertility, or abortion of the fetus, respectively. Here, exposure of primary bovine endometrial cells to BoHV-4 or BVDV modulated the production of inflammatory mediators. Viral pathogen-associated molecular patterns (PAMPs) are detected via pattern-recognition receptors (PRRs). However, the relative contribution of specific PRRs to innate immunity, during viral infection of the uterus, is unclear. Endometrial epithelial and stromal cells constitutively express the PRR Toll-like receptor (TLR)-3, but, the status of retinoic acid-inducible gene I (RIG-I), a sensor of cytosolic nucleic acids, is unknown. Primary endometrial epithelial and stromal cells had low expression of RIG-I, which was increased in stromal cells after 12 h transfection with the TLR3 ligand Poly(I:C), a synthetic analog of double-stranded RNA. Furthermore, short interfering RNA targeting TLR3, or interferon (IFN) regulatory transcription factor 3, an inducer of type I IFN transcription, reduced Poly(I:C)-induced RIG-I protein expression and reduced inflammatory mediator secretion from stromal cells. We conclude that antiviral defense of endometrial stromal cells requires coordinated recognition of PAMPs, initially via TLR3 and later via inducible RIG-I.
published_date 2017-08-23T03:43:25Z
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