No Cover Image

Journal article 409 views

Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome / Virginia Post; Llinos Harris; Mario Morgenstern; Leonardos Mageiros; Matthew Hitchings; Guillaume Méric; Ben Pascoe; Samuel K. Sheppard; R. Geoff Richards; T. Fintan Moriarty

Journal of Clinical Microbiology, Volume: 55, Issue: 10, Pages: 3089 - 3103

Swansea University Authors: Llinos, Harris, Matthew, Hitchings

Full text not available from this repository: check for access using links below.

Check full text

DOI (Published version): 10.1128/JCM.00881-17

Abstract

Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic-device-related infections (ODRI). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome for the infected pa...

Full description

Published in: Journal of Clinical Microbiology
ISSN: 0095-1137 1098-660X
Published: 2017
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa35058
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic-device-related infections (ODRI). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome for the infected patient. S. epidermidis isolates were collected from 104 patients with ODRI. Their clinical outcomes were evaluated, after an average of 26 months, as either “cured” or “not cured.” The isolates were tested for antibiotic susceptibility and biofilm formation. Whole-genome sequencing was performed on all isolates, and genomic variation was related to features associated with “cured” and “not cured.” Strong biofilm formation and aminoglycoside resistance were associated with a “not-cured” outcome (P = 0.031 and P = 0.001, respectively). Based on gene-by-gene analysis, some accessory genes were more prevalent in isolates from the “not-cured” group. These included the biofilm-associated bhp gene, the antiseptic resistance qacA gene, the cassette chromosome recombinase-encoding genes ccrA and ccrB, and the IS256-like transposase gene. This study identifies biofilm formation and antibiotic resistance as associated with poor outcome in S. epidermidis ODRI. Whole-genome sequencing identified specific genes associated with a “not-cured” outcome that should be validated in future studies. (The study has been registered at ClinicalTrials.govwith identifier NCT02640937.)
Keywords: Staphylococcus epidermidis; MRSE; virulence factors; antibiotic resistance; genotype; phenotype; orthopedic device-related infections
College: Swansea University Medical School
Issue: 10
Start Page: 3089
End Page: 3103