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Comparison of the composition of bile acids in bile of patients with adenocarcinoma of the pancreas and benign disease

David O. Rees, Peter J. Crick, Gareth Jenkins Orcid Logo, Yuqin Wang Orcid Logo, William Griffiths Orcid Logo, Tim H. Brown, Bilal Al-Sarireh

The Journal of Steroid Biochemistry and Molecular Biology, Volume: 174, Pages: 290 - 295

Swansea University Authors: Gareth Jenkins Orcid Logo, Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

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DOI (Published version): 10.1016/j.jsbmb.2017.10.011

Abstract

Bile acids have been implicated in the development of gastrointestinal malignancies. Both the specific nature of individual bile acids and their concentration appear key factors in the carcinogenic potency of bile. Using liquid chromatography mass spectrometry (LC-MS) we performed quantitative profi...

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Published in: The Journal of Steroid Biochemistry and Molecular Biology
ISSN: 09600760
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa36199
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spelling 2020-06-03T13:19:41.3812388 v2 36199 2017-10-23 Comparison of the composition of bile acids in bile of patients with adenocarcinoma of the pancreas and benign disease a44095d26187304e903da7ca778697b6 0000-0002-5437-8389 Gareth Jenkins Gareth Jenkins true false c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2017-10-23 BMS Bile acids have been implicated in the development of gastrointestinal malignancies. Both the specific nature of individual bile acids and their concentration appear key factors in the carcinogenic potency of bile. Using liquid chromatography mass spectrometry (LC-MS) we performed quantitative profiling of bile extracted directly from the common bile duct in 30 patients (15 patients with pancreatic cancer and 15 patients with benign disease). Separation and detection of bile acids was performed using a 1.7μm particle size reversed-phase C18 LC column at a flow rate of 200μL/min with negative electrospray ionization MS. A significant difference (p=0.018) was seen in the concentration of unconjugated cholic acid in the malignant group (0.643mmol/L) compared to the benign group (0.022mmol/L), with an overall significant difference (p=0.04) seen in the level of total unconjugated bile acids in the malignant group (1.816mmol/L) compared to the benign group (0.069mmol/L). This finding may offer the possibility of both understanding the biology of cancer development in the pancreas, as well as offering a potential diagnostic avenue to explore. However, a larger study is necessary to confirm the alterations in bile acid profiles reported here and explore factors such as diet and microbial populations on the bile acid profiles of these patient groups. Journal Article The Journal of Steroid Biochemistry and Molecular Biology 174 290 295 09600760 pancreatic cancer, liquid chromatography–mass spectrometry, bile. bile acids 1 11 2017 2017-11-01 10.1016/j.jsbmb.2017.10.011 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University RCUK, BBSRC, BB/I001735/1 2020-06-03T13:19:41.3812388 2017-10-23T11:40:58.1999312 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine David O. Rees 1 Peter J. Crick 2 Gareth Jenkins 0000-0002-5437-8389 3 Yuqin Wang 0000-0002-3063-3066 4 William Griffiths 0000-0002-4129-6616 5 Tim H. Brown 6 Bilal Al-Sarireh 7 0036199-05112017155836.pdf ReesJSBMB1742017290.pdf 2017-11-05T15:58:36.5300000 Output 282665 application/pdf Version of Record true 2017-11-05T00:00:00.0000000 Open Access funded by Biotechnology and Biological Sciences Research Council. Under a Creative Commons license. true eng
title Comparison of the composition of bile acids in bile of patients with adenocarcinoma of the pancreas and benign disease
spellingShingle Comparison of the composition of bile acids in bile of patients with adenocarcinoma of the pancreas and benign disease
Gareth Jenkins
Yuqin Wang
William Griffiths
title_short Comparison of the composition of bile acids in bile of patients with adenocarcinoma of the pancreas and benign disease
title_full Comparison of the composition of bile acids in bile of patients with adenocarcinoma of the pancreas and benign disease
title_fullStr Comparison of the composition of bile acids in bile of patients with adenocarcinoma of the pancreas and benign disease
title_full_unstemmed Comparison of the composition of bile acids in bile of patients with adenocarcinoma of the pancreas and benign disease
title_sort Comparison of the composition of bile acids in bile of patients with adenocarcinoma of the pancreas and benign disease
author_id_str_mv a44095d26187304e903da7ca778697b6
c92729b58622f9fdf6a0e7d8f4ce5081
3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv a44095d26187304e903da7ca778697b6_***_Gareth Jenkins
c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang
3316b1d1b524be1831790933eed1c26e_***_William Griffiths
author Gareth Jenkins
Yuqin Wang
William Griffiths
author2 David O. Rees
Peter J. Crick
Gareth Jenkins
Yuqin Wang
William Griffiths
Tim H. Brown
Bilal Al-Sarireh
format Journal article
container_title The Journal of Steroid Biochemistry and Molecular Biology
container_volume 174
container_start_page 290
publishDate 2017
institution Swansea University
issn 09600760
doi_str_mv 10.1016/j.jsbmb.2017.10.011
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
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description Bile acids have been implicated in the development of gastrointestinal malignancies. Both the specific nature of individual bile acids and their concentration appear key factors in the carcinogenic potency of bile. Using liquid chromatography mass spectrometry (LC-MS) we performed quantitative profiling of bile extracted directly from the common bile duct in 30 patients (15 patients with pancreatic cancer and 15 patients with benign disease). Separation and detection of bile acids was performed using a 1.7μm particle size reversed-phase C18 LC column at a flow rate of 200μL/min with negative electrospray ionization MS. A significant difference (p=0.018) was seen in the concentration of unconjugated cholic acid in the malignant group (0.643mmol/L) compared to the benign group (0.022mmol/L), with an overall significant difference (p=0.04) seen in the level of total unconjugated bile acids in the malignant group (1.816mmol/L) compared to the benign group (0.069mmol/L). This finding may offer the possibility of both understanding the biology of cancer development in the pancreas, as well as offering a potential diagnostic avenue to explore. However, a larger study is necessary to confirm the alterations in bile acid profiles reported here and explore factors such as diet and microbial populations on the bile acid profiles of these patient groups.
published_date 2017-11-01T03:45:12Z
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