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Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro / InaLaura Pieper; Gemma Radley; Abigail Christen; Sabrina Ali; Owen Bodger; Catherine Thornton

Artificial Organs

Swansea University Author: Thornton, Catherine

DOI (Published version): 10.1111/aor.13068

Abstract

Ventricular assist devices (VADs) are a life-saving form of mechanical circulatory support in heart failure patients. However, VADs have not yet reached their full potential due to the associated side effects (thrombosis, bleeding, infection) related to the activation and damage of blood cells and p...

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Published in: Artificial Organs
Published: 2018
URI: https://cronfa.swan.ac.uk/Record/cronfa36276
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first_indexed 2017-10-26T13:34:23Z
last_indexed 2018-07-10T13:22:27Z
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spelling 2018-07-09T16:54:52Z v2 36276 2017-10-26 Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro Catherine Thornton Catherine Thornton true 0000-0002-5153-573X false c71a7a4be7361094d046d312202bce0c 0789291f2bbb8aeb948db2352e425e6a 70js5AkMS5jPo3arCUY9c32HZhUyFASdV1DFdgIIhKs= 2017-10-26 PMSC Ventricular assist devices (VADs) are a life-saving form of mechanical circulatory support in heart failure patients. However, VADs have not yet reached their full potential due to the associated side effects (thrombosis, bleeding, infection) related to the activation and damage of blood cells and proteins caused by mechanical stress and foreign materials. Studies of the effects of VADs on leukocytes are limited, yet leukocyte activation and damage including microparticle generation can influence both thrombosis and infection rates. Therefore, the aim was to develop a multicolour flow cytometry assessment of leukocyte microparticles (LMPs) using ovine blood and the CentriMag VAD as a model for shear stress. Ovine blood was pumped for 6 hours in the CentriMag and regular samples analysed for haemolysis, complete blood counts and leukocyte microparticles by flow cytometry during three different pump operating conditions (low flow, standard, high speed). The high speed condition caused significant increases in plasma-free haemoglobin; decreases in total leukocytes, granulocytes, monocytes and platelets; increases in CD45+ LMPs as well as two novel LMP populations: CD11bbright/HLA-DR- and CD11bdull/HLA-DR+, both of which were CD14-/CD21-. CD11bbright/HLA-DR- LMPs appeared to respond to an increase in shear magnitude whereas the CD11bdull/HLA-DR+ LMPs significantly increased in all pumping conditions. We propose that these two populations are released from granulocytes and T cells, respectively, but further research is needed to better characterise these two populations. Journal article Artificial Organs Flow cytometry, ovine, leukocyte, microparticle, shear stress, ventricular assist, CD11b, HLA-DR, CentriMag 7 3 2018 2018-03-07 10.1111/aor.13068 Swansea University Medical School Medicine CMED PMSC Microbes and immunity None 2018-07-09T16:54:52Z 2017-10-26T11:23:57Z Swansea University Medical School Medicine InaLaura Pieper 1 Gemma Radley 2 Abigail Christen 3 Sabrina Ali 4 Owen Bodger 5 Catherine Thornton 6 0036276-17052018164204.pdf OvineactivatedMPsintheCMAG_FinalforSUMSv2.pdf 2018-05-17T16:42:04Z Output 1893252 application/pdf AM true Published to Cronfa 17/05/2018 2019-03-07T00:00:00 true eng
title Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
spellingShingle Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
Thornton, Catherine
title_short Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
title_full Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
title_fullStr Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
title_full_unstemmed Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
title_sort Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
author_id_str_mv c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv c71a7a4be7361094d046d312202bce0c_***_Thornton, Catherine
author Thornton, Catherine
author2 InaLaura Pieper
Gemma Radley
Abigail Christen
Sabrina Ali
Owen Bodger
Catherine Thornton
format Journal article
container_title Artificial Organs
publishDate 2018
institution Swansea University
doi_str_mv 10.1111/aor.13068
college_str Swansea University Medical School
hierarchytype
hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 1
active_str 1
researchgroup_str Microbes and immunity
description Ventricular assist devices (VADs) are a life-saving form of mechanical circulatory support in heart failure patients. However, VADs have not yet reached their full potential due to the associated side effects (thrombosis, bleeding, infection) related to the activation and damage of blood cells and proteins caused by mechanical stress and foreign materials. Studies of the effects of VADs on leukocytes are limited, yet leukocyte activation and damage including microparticle generation can influence both thrombosis and infection rates. Therefore, the aim was to develop a multicolour flow cytometry assessment of leukocyte microparticles (LMPs) using ovine blood and the CentriMag VAD as a model for shear stress. Ovine blood was pumped for 6 hours in the CentriMag and regular samples analysed for haemolysis, complete blood counts and leukocyte microparticles by flow cytometry during three different pump operating conditions (low flow, standard, high speed). The high speed condition caused significant increases in plasma-free haemoglobin; decreases in total leukocytes, granulocytes, monocytes and platelets; increases in CD45+ LMPs as well as two novel LMP populations: CD11bbright/HLA-DR- and CD11bdull/HLA-DR+, both of which were CD14-/CD21-. CD11bbright/HLA-DR- LMPs appeared to respond to an increase in shear magnitude whereas the CD11bdull/HLA-DR+ LMPs significantly increased in all pumping conditions. We propose that these two populations are released from granulocytes and T cells, respectively, but further research is needed to better characterise these two populations.
published_date 2018-03-07T13:32:01Z
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