“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel

Giudice, Francesco Del; Sathish, Shivani; D’Avino, Gaetano; Shen, Amy Q.

doi:10.1021/acs.analchem.7b02450

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“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel

Francesco Del Giudice

, Shivani Sathish, Gaetano D’Avino, Amy Q. Shen

Analytical Chemistry, Volume: 89, Issue: 24, Pages: 13146 - 13159

Swansea University Author: Francesco Del Giudice

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DOI (Published version): 10.1021/acs.analchem.7b02450

Abstract

Controlling the fate of particles and cells in microfluidic devices is critical in many biomedical applications, such as particle and cell alignment and separation. Recently, viscoelastic polymer solutions have been successfully used to promote transversal migration of particles and cells toward fix...

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Published in:	Analytical Chemistry
ISSN:	0003-2700 1520-6882
Published:	American Chemical Society (ACS) 2017
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URI:	https://cronfa.swan.ac.uk/Record/cronfa41015
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first_indexed	2018-07-13T19:35:36Z
last_indexed	2020-09-30T03:02:16Z
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spelling	2020-09-29T18:33:21.6393013 v2 41015 2018-07-13 “From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel 742d483071479b44d7888e16166b1309 0000-0002-9414-6937 Francesco Del Giudice Francesco Del Giudice true false 2018-07-13 CHEG Controlling the fate of particles and cells in microfluidic devices is critical in many biomedical applications, such as particle and cell alignment and separation. Recently, viscoelastic polymer solutions have been successfully used to promote transversal migration of particles and cells toward fixed positions in straight microchannels. When inertia is negligible, numerical simulations have shown that strongly shear-thinning polymer solutions (fluids with a shear viscosity that decreases with increasing flow rates) promote transversal migration of particles and cells toward the corners or toward the centerline in a straight microchannel with a square cross section, as a function of particle size, cell deformability, and channel height. However, no experimental evidence of such shifting in the positions for particles or cells suspended in strongly shear-thinning liquids has been presented so far. In this work, we demonstrate that particle positions over the channel cross section can be shifted “from the edge to the center” in a strongly shear-thinning liquid. We investigate the viscoelasticity-induced migration of both rigid particles and living cells (Jurkat cells and NIH 3T3 fibroblasts) in an aqueous 0.8 wt % hyaluronic acid solution. The combined effect of fluid elasticity, shear-thinning, geometric confinement, and cell deformability on the distribution of the particle/cell positions over the channel cross section is presented and discussed. In the same shear-thinning liquid, separation of 10 and 20 μm particles is also achieved in a straight microchannel with an abrupt expansion. Our results envisage further applications in viscoelasticity-based microfluidics, such as deformability-based cell separation and viscoelastic spacing of particles/cells. Journal Article Analytical Chemistry 89 24 13146 13159 American Chemical Society (ACS) 0003-2700 1520-6882 19 12 2017 2017-12-19 10.1021/acs.analchem.7b02450 http://dx.doi.org/10.1021/acs.analchem.7b02450 COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University 2020-09-29T18:33:21.6393013 2018-07-13T14:45:51.8672798 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Francesco Del Giudice 0000-0002-9414-6937 1 Shivani Sathish 2 Gaetano D’Avino 3 Amy Q. Shen 4 0041015-31082018113223.pdf DelGiudice.pdf 2018-08-31T11:32:23.2470000 Output 3701683 application/pdf Version of Record true 2018-08-31T00:00:00.0000000 This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. true eng
title	“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel
spellingShingle	“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel Francesco Del Giudice
title_short	“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel
title_full	“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel
title_fullStr	“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel
title_full_unstemmed	“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel
title_sort	“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel
author_id_str_mv	742d483071479b44d7888e16166b1309
author_id_fullname_str_mv	742d483071479b44d7888e16166b1309_***_Francesco Del Giudice
author	Francesco Del Giudice
author2	Francesco Del Giudice Shivani Sathish Gaetano D’Avino Amy Q. Shen
format	Journal article
container_title	Analytical Chemistry
container_volume	89
container_issue	24
container_start_page	13146
publishDate	2017
institution	Swansea University
issn	0003-2700 1520-6882
doi_str_mv	10.1021/acs.analchem.7b02450
publisher	American Chemical Society (ACS)
college_str	Faculty of Science and Engineering
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url	http://dx.doi.org/10.1021/acs.analchem.7b02450
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description	Controlling the fate of particles and cells in microfluidic devices is critical in many biomedical applications, such as particle and cell alignment and separation. Recently, viscoelastic polymer solutions have been successfully used to promote transversal migration of particles and cells toward fixed positions in straight microchannels. When inertia is negligible, numerical simulations have shown that strongly shear-thinning polymer solutions (fluids with a shear viscosity that decreases with increasing flow rates) promote transversal migration of particles and cells toward the corners or toward the centerline in a straight microchannel with a square cross section, as a function of particle size, cell deformability, and channel height. However, no experimental evidence of such shifting in the positions for particles or cells suspended in strongly shear-thinning liquids has been presented so far. In this work, we demonstrate that particle positions over the channel cross section can be shifted “from the edge to the center” in a strongly shear-thinning liquid. We investigate the viscoelasticity-induced migration of both rigid particles and living cells (Jurkat cells and NIH 3T3 fibroblasts) in an aqueous 0.8 wt % hyaluronic acid solution. The combined effect of fluid elasticity, shear-thinning, geometric confinement, and cell deformability on the distribution of the particle/cell positions over the channel cross section is presented and discussed. In the same shear-thinning liquid, separation of 10 and 20 μm particles is also achieved in a straight microchannel with an abrupt expansion. Our results envisage further applications in viscoelasticity-based microfluidics, such as deformability-based cell separation and viscoelastic spacing of particles/cells.
published_date	2017-12-19T03:52:16Z
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“From the Edge to the Center”: Viscoelastic Migration of Particles and Cells in a Strongly Shear-Thinning Liquid Flowing in a Microchannel

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