No Cover Image

Journal article 178 views 15 downloads

Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model / F. Ahmad; S. Soe; N. White; R. Johnston; I. Khan; J. Liao; M. Jones; R. Prabhu; I. Maconochie; Peter Theobald

Annals of Biomedical Engineering

Swansea University Author: Khan, Ilyas

  • 41043.pdf

    PDF | Version of Record

    This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.

    Download (5.17MB)

Abstract

The neonate transitions from placenta-derived oxygen, to supply from the pulmonary system, moments after birth. This requires a series of structural developments to divert more blood through the right heart and onto the lungs, with the tissue quickly remodelling to the changing ventricular workload....

Full description

Published in: Annals of Biomedical Engineering
ISSN: 0090-6964 1573-9686
Published: 2018
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa41043
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: The neonate transitions from placenta-derived oxygen, to supply from the pulmonary system, moments after birth. This requires a series of structural developments to divert more blood through the right heart and onto the lungs, with the tissue quickly remodelling to the changing ventricular workload. In some cases, however, the heart structure does not fully develop causing poor circulation and inefficient oxygenation, which is associated with an increase in mortality and morbidity. This study focuses on developing an enhanced knowledge of the 1-day old heart, quantifying the region-specific microstructural parameters of the tissue. This will enable more accurate mathematical and computa- tional simulations of the young heart. Hearts were dissected from 12, 1-day-old deceased Yorkshire piglets (mass: 2.1– 2.4 kg, length: 0.38–0.51 m), acquired from a breeding farm. Evans blue dye was used to label the heart equator and to demarcate the left and right ventricle free walls. Two hearts were used for three-dimensional diffusion-tensor magnetic resonance imaging, to quantify the fractional anisotropy (FA). The remaining hearts were used for two-photon excited fluorescence and second-harmonic generation microscopy, to quantify the cardiomyocyte and collagen fibril structures within the anterior and posterior aspects of the right and left ventricles. FA varied significantly across both ventricles, with the greatest in the equatorial region, followed by the base and apex. The FA in each right ventricular region was statistically greater than that in the left. Cardiomyocyte and collagen fibre rotation was greatest in the anterior wall of both ventricles, with less dispersion when compared to the posterior walls. In defining these key parameters, this study provides a valuable insight into the 1-day-old heart that will provide a valuable platform for further investigation the normal and abnormal heart using mathematical and com- putational models.
College: Swansea University Medical School