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Study of the correlation between archived metal-polluted soils and microbial resistance to antibiotics and metals / Alyaa Abdelhameed

Swansea University Author: Alyaa Abdelhameed

DOI (Published version): 10.23889/Suthesis.41112

Abstract

Soil contamination with metals is a widespread problem, prominent near mines and industrial facilities. The British Geology Survey via its Geochemical Baseline Survey of Environment (G-BASE) project provides a resource of well-documented soil archives from national and international locations, givin...

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Published: Swansea 2018
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa41112
first_indexed 2018-07-24T12:17:36Z
last_indexed 2024-11-14T11:50:22Z
id cronfa41112
recordtype RisThesis
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spelling 2024-07-11T14:40:11.8198561 v2 41112 2018-07-24 Study of the correlation between archived metal-polluted soils and microbial resistance to antibiotics and metals 254a0d46002ee430b6e7d71aca7ed1bc NULL Alyaa Abdelhameed Alyaa Abdelhameed true true 2018-07-24 Soil contamination with metals is a widespread problem, prominent near mines and industrial facilities. The British Geology Survey via its Geochemical Baseline Survey of Environment (G-BASE) project provides a resource of well-documented soil archives from national and international locations, giving geochemical datasets that comprise metal concentrations in top and profile soil samples. This study focused on G-BASE data and soil samples collected from urban areas of Swansea-Neath-Port Talbot (SNPT) in South Wales (UK), with sampling points at a density of 1 per 0.25km2.This PhD study has evaluated (i) the pollution status of metals in urban soil samples and their potential ecological risk in an urban UK environment, specifically the SNPT area;(ii) the effect of metals on bacterial community and diversity in metal-polluted urbansoils, and (iii) the effect of metals on microbial adaptation to antimicrobial stress and resistance and on genome evolution. A representative selection of soil samples from the G-BASE soil collection was tested to enable completion of culture-independent and culture-dependent ecological studies.The pollution status in the 373 sampled soils was assessed using the pollution index of metals and correlations between metals were assessed. The bacterial community structure was studied in 63 soil samples which evaluated the effect of 16 metals on microbial community composition and diversity. The effect of metal pollution stress on bacterial viability in soils was investigated for resistance to four common antibiotics. Bacterial adaptation to metal pollution and AMR in metal-antibiotic co-exposure responses was also tested in clinically relevant pathogenic bacteria, specifically methicillin-sensitive and -resistant Staphylococcus aureus to identify routes for antimicrobial chemotherapeutic drug discovery. Finally, the effect of metal exposure on the dynamics of genomic adaptation and resistance in bacterial isolates was determined by identification of pan- and accessory genomes and resistome marker genes through whole genome sequencing and bioinformatic analysis. E-Thesis Swansea Soil, soil contamination, industrial pollution, heavy metals, environment, geochemical, South Wales 31 12 2018 2018-12-31 10.23889/Suthesis.41112 A selection of third party content is redacted or is partially redacted from this thesis. COLLEGE NANME Swansea University Medical School COLLEGE CODE Swansea University Doctoral Ph.D 2024-07-11T14:40:11.8198561 2018-07-24T11:18:44.2392180 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Alyaa Abdelhameed NULL 1 0041112-24072018120854.pdf Abdelhameed,__Alyaa___thesis___Redacted.2018.pdf 2018-07-24T12:08:54.5570000 Output 14550309 application/pdf Redacted version - open access true 2023-07-26T00:00:00.0000000 true eng
title Study of the correlation between archived metal-polluted soils and microbial resistance to antibiotics and metals
spellingShingle Study of the correlation between archived metal-polluted soils and microbial resistance to antibiotics and metals
Alyaa Abdelhameed
title_short Study of the correlation between archived metal-polluted soils and microbial resistance to antibiotics and metals
title_full Study of the correlation between archived metal-polluted soils and microbial resistance to antibiotics and metals
title_fullStr Study of the correlation between archived metal-polluted soils and microbial resistance to antibiotics and metals
title_full_unstemmed Study of the correlation between archived metal-polluted soils and microbial resistance to antibiotics and metals
title_sort Study of the correlation between archived metal-polluted soils and microbial resistance to antibiotics and metals
author_id_str_mv 254a0d46002ee430b6e7d71aca7ed1bc
author_id_fullname_str_mv 254a0d46002ee430b6e7d71aca7ed1bc_***_Alyaa Abdelhameed
author Alyaa Abdelhameed
author2 Alyaa Abdelhameed
format E-Thesis
publishDate 2018
institution Swansea University
doi_str_mv 10.23889/Suthesis.41112
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Soil contamination with metals is a widespread problem, prominent near mines and industrial facilities. The British Geology Survey via its Geochemical Baseline Survey of Environment (G-BASE) project provides a resource of well-documented soil archives from national and international locations, giving geochemical datasets that comprise metal concentrations in top and profile soil samples. This study focused on G-BASE data and soil samples collected from urban areas of Swansea-Neath-Port Talbot (SNPT) in South Wales (UK), with sampling points at a density of 1 per 0.25km2.This PhD study has evaluated (i) the pollution status of metals in urban soil samples and their potential ecological risk in an urban UK environment, specifically the SNPT area;(ii) the effect of metals on bacterial community and diversity in metal-polluted urbansoils, and (iii) the effect of metals on microbial adaptation to antimicrobial stress and resistance and on genome evolution. A representative selection of soil samples from the G-BASE soil collection was tested to enable completion of culture-independent and culture-dependent ecological studies.The pollution status in the 373 sampled soils was assessed using the pollution index of metals and correlations between metals were assessed. The bacterial community structure was studied in 63 soil samples which evaluated the effect of 16 metals on microbial community composition and diversity. The effect of metal pollution stress on bacterial viability in soils was investigated for resistance to four common antibiotics. Bacterial adaptation to metal pollution and AMR in metal-antibiotic co-exposure responses was also tested in clinically relevant pathogenic bacteria, specifically methicillin-sensitive and -resistant Staphylococcus aureus to identify routes for antimicrobial chemotherapeutic drug discovery. Finally, the effect of metal exposure on the dynamics of genomic adaptation and resistance in bacterial isolates was determined by identification of pan- and accessory genomes and resistome marker genes through whole genome sequencing and bioinformatic analysis.
published_date 2018-12-31T04:20:42Z
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score 11.090445

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