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Investigations of androgenic steroids by liquid chromatography / mass spectrometry. / Jinglei Yu

Swansea University Author: Jinglei Yu

Abstract

Androgen steroids are an important group in the hormone family. As they play significant roles in the human body, identification and determination of these compounds have been of great interest in many fields. In this thesis, systematic studies of the compounds in the androgen group by liquid chroma...

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Published: 2004
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa42641
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first_indexed 2018-08-02T18:55:11Z
last_indexed 2018-08-03T10:10:41Z
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spelling 2018-08-02T16:24:29.9461990 v2 42641 2018-08-02 Investigations of androgenic steroids by liquid chromatography / mass spectrometry. 471b5adc97cab0d793dfe3acd48c0ccd NULL Jinglei Yu Jinglei Yu true true 2018-08-02 Androgen steroids are an important group in the hormone family. As they play significant roles in the human body, identification and determination of these compounds have been of great interest in many fields. In this thesis, systematic studies of the compounds in the androgen group by liquid chromatography / mass spectrometry have been undertaken. The first chapter gives a brief introduction to the structure, function and classification of the steroid family, and is followed by a review of different analytical techniques applied in this family. The theory behind the chromatographic and mass spectrometric techniques used has also been introduced. Structural information about the androgenic steroids and their conjugates has been obtained in chapters 2 and 3 respectively by both ESI and APCI mass spectrometry. The effect of different solvent systems was studied in detail in both the positive and negative ionization modes and the collision induced dissociation of these compounds was also investigated by tandem mass spectrometry. In chapter 4, LC/MS methods have been optimized in terms of columns, solvent systems, flow rate and mass spectrometer parameters to quantify some of the androgens and their conjugates respectively. The linearity, precision and limits of detection were estimated for these compounds. A derivatization method was developed to enhance the sensitivities for detection of the androgens and their conjugates by LC/MS in the final chapter. A TMPP reagent was used as a derivatizing reagent, and the spectra of these TMPP-derivatives of androgens were studied by ESI mass spectrometry. A LC/MS method was also set up with the aim of quantitation of five TMPP-derivatives of androgens. E-Thesis Analytical chemistry. 31 12 2004 2004-12-31 COLLEGE NANME Chemistry COLLEGE CODE Swansea University Doctoral Ph.D 2018-08-02T16:24:29.9461990 2018-08-02T16:24:29.9461990 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemistry Jinglei Yu NULL 1 0042641-02082018162510.pdf 10805417.pdf 2018-08-02T16:25:10.4600000 Output 14489784 application/pdf E-Thesis true 2018-08-02T16:25:10.4600000 false
title Investigations of androgenic steroids by liquid chromatography / mass spectrometry.
spellingShingle Investigations of androgenic steroids by liquid chromatography / mass spectrometry.
Jinglei Yu
title_short Investigations of androgenic steroids by liquid chromatography / mass spectrometry.
title_full Investigations of androgenic steroids by liquid chromatography / mass spectrometry.
title_fullStr Investigations of androgenic steroids by liquid chromatography / mass spectrometry.
title_full_unstemmed Investigations of androgenic steroids by liquid chromatography / mass spectrometry.
title_sort Investigations of androgenic steroids by liquid chromatography / mass spectrometry.
author_id_str_mv 471b5adc97cab0d793dfe3acd48c0ccd
author_id_fullname_str_mv 471b5adc97cab0d793dfe3acd48c0ccd_***_Jinglei Yu
author Jinglei Yu
author2 Jinglei Yu
format E-Thesis
publishDate 2004
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemistry{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemistry
document_store_str 1
active_str 0
description Androgen steroids are an important group in the hormone family. As they play significant roles in the human body, identification and determination of these compounds have been of great interest in many fields. In this thesis, systematic studies of the compounds in the androgen group by liquid chromatography / mass spectrometry have been undertaken. The first chapter gives a brief introduction to the structure, function and classification of the steroid family, and is followed by a review of different analytical techniques applied in this family. The theory behind the chromatographic and mass spectrometric techniques used has also been introduced. Structural information about the androgenic steroids and their conjugates has been obtained in chapters 2 and 3 respectively by both ESI and APCI mass spectrometry. The effect of different solvent systems was studied in detail in both the positive and negative ionization modes and the collision induced dissociation of these compounds was also investigated by tandem mass spectrometry. In chapter 4, LC/MS methods have been optimized in terms of columns, solvent systems, flow rate and mass spectrometer parameters to quantify some of the androgens and their conjugates respectively. The linearity, precision and limits of detection were estimated for these compounds. A derivatization method was developed to enhance the sensitivities for detection of the androgens and their conjugates by LC/MS in the final chapter. A TMPP reagent was used as a derivatizing reagent, and the spectra of these TMPP-derivatives of androgens were studied by ESI mass spectrometry. A LC/MS method was also set up with the aim of quantitation of five TMPP-derivatives of androgens.
published_date 2004-12-31T03:53:22Z
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score 11.035655

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