A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens

Terra, Luciana; Dyson, Paul J.; Hitchings, Matthew; Thomas, Liam; Abdelhameed, Alyaa; Banat, Ibrahim M.; Gazze, Salvatore A.; Vujaklija, Dušica; Facey, Paul; Francis, Lewis; Quinn, Gerry A.; Dyson, Paul

doi:10.3389/fmicb.2018.02458

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A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens

Luciana Terra, Paul J. Dyson, Matthew Hitchings

, Liam Thomas, Alyaa Abdelhameed, Ibrahim M. Banat, Salvatore A. Gazze, Dušica Vujaklija, Paul Facey

, Lewis Francis

, Gerry A. Quinn, Paul Dyson

Frontiers in Microbiology, Volume: 9

Swansea University Authors: Matthew Hitchings , Paul Facey , Lewis Francis , Paul Dyson

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DOI (Published version): 10.3389/fmicb.2018.02458

Abstract

In an effort to stem the rising tide of multi-resistant bacteria, researchers have turned to niche environments in the hope of discovering new varieties of antibiotics. We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ire...

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Published in:	Frontiers in Microbiology
ISSN:	1664-302X
Published:	Frontiers Media SA 2018
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa44961
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first_indexed	2018-10-18T13:20:34Z
last_indexed	2021-01-28T04:06:17Z
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We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ireland) for the presence of Streptomyces, a well-known producer of antibiotics. From this soil we isolated a novel (closest relative 57% of genome relatedness) Streptomyces sp. capable of growth at high alkaline pH (10.5) and tolerant of gamma radiation to 4 kGy. Genomic sequencing identified many alkaline tolerance (antiporter/multi-resistance) genes compared to S. coelicolor M145 (at 3:1), hence we designated the strain Streptomyces sp. myrophorea, isolate McG1, from the Greek, myro (fragrance) and phorea (porter/carrier). In vitro tests demonstrated the ability of the Streptomyces sp. myrophorea, isolate McG1 to inhibit the growth of many strains of ESKAPE pathogens; most notably carbapenem-resistant Acinetobacter baumannii (a critical pathogen on the WHO priority list of antibiotic-resistant bacteria), vancomycin-resistant Enterococcus faecium, and methicillin-resistant Staphylococcus aureus (both listed as high priority pathogens). Further in silico prediction of antimicrobial potential of Streptomyces sp. myrophorea, isolate McG1 by anti-SMASH and RAST software identified many secondary metabolite and toxicity resistance gene clusters (45 and 27, respectively) as well as many antibiotic resistance genes potentially related to antibiotic production. Follow-up in vitro tests show that the Streptomyces sp. myrophorea, isolate McG1 was resistant to 28 out of 36 clinical antibiotics. Although not a comprehensive analysis, we think that some of the Boho soils’ reputed curative properties may be linked to the ability of Streptomyces sp. myrophorea, isolate McG1 to inhibit ESKAPE pathogens. More importantly, further analysis may elucidate other key components that could alleviate the tide of multi-resistant nosocomial infections.</abstract><type>Journal Article</type><journal>Frontiers in Microbiology</journal><volume>9</volume><journalNumber/><paginationStart/><paginationEnd/><publisher>Frontiers Media SA</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>1664-302X</issnElectronic><keywords/><publishedDay>16</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2018</publishedYear><publishedDate>2018-10-16</publishedDate><doi>10.3389/fmicb.2018.02458</doi><url>http://dx.doi.org/10.3389/fmicb.2018.02458</url><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2021-01-27T17:58:27.9727522</lastEdited><Created>2018-10-18T11:31:06.2282011</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Luciana</firstname><surname>Terra</surname><order>1</order></author><author><firstname>Paul J.</firstname><surname>Dyson</surname><order>2</order></author><author><firstname>Matthew</firstname><surname>Hitchings</surname><orcid>0000-0002-5527-4709</orcid><order>3</order></author><author><firstname>Liam</firstname><surname>Thomas</surname><order>4</order></author><author><firstname>Alyaa</firstname><surname>Abdelhameed</surname><order>5</order></author><author><firstname>Ibrahim M.</firstname><surname>Banat</surname><order>6</order></author><author><firstname>Salvatore A.</firstname><surname>Gazze</surname><order>7</order></author><author><firstname>Dušica</firstname><surname>Vujaklija</surname><order>8</order></author><author><firstname>Paul</firstname><surname>Facey</surname><orcid>0000-0002-3229-0255</orcid><order>9</order></author><author><firstname>Lewis</firstname><surname>Francis</surname><orcid>0000-0002-7803-7714</orcid><order>10</order></author><author><firstname>Gerry A.</firstname><surname>Quinn</surname><order>11</order></author><author><firstname>Paul</firstname><surname>Dyson</surname><orcid>0000-0002-0558-2666</orcid><order>12</order></author></authors><documents><document><filename>0044961-05112018102706.pdf</filename><originalFilename>44961.pdf</originalFilename><uploaded>2018-11-05T10:27:06.9030000</uploaded><type>Output</type><contentLength>2605691</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2018-11-04T00:00:00.0000000</embargoDate><documentNotes>Released under the terms of the Creative Commons Attribution License (CC-BY).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling	2021-01-27T17:58:27.9727522 v2 44961 2018-10-18 A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens be98847c72c14a731c4a6b7bc02b3bcf 0000-0002-5527-4709 Matthew Hitchings Matthew Hitchings true false dc25910b8004b2694df68ed7426e1286 0000-0002-3229-0255 Paul Facey Paul Facey true false 10f61f9c1248951c1a33f6a89498f37d 0000-0002-7803-7714 Lewis Francis Lewis Francis true false 300e3f46b70ae83f563b24f41d00cd17 0000-0002-0558-2666 Paul Dyson Paul Dyson true false 2018-10-18 BMS In an effort to stem the rising tide of multi-resistant bacteria, researchers have turned to niche environments in the hope of discovering new varieties of antibiotics. We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ireland) for the presence of Streptomyces, a well-known producer of antibiotics. From this soil we isolated a novel (closest relative 57% of genome relatedness) Streptomyces sp. capable of growth at high alkaline pH (10.5) and tolerant of gamma radiation to 4 kGy. Genomic sequencing identified many alkaline tolerance (antiporter/multi-resistance) genes compared to S. coelicolor M145 (at 3:1), hence we designated the strain Streptomyces sp. myrophorea, isolate McG1, from the Greek, myro (fragrance) and phorea (porter/carrier). In vitro tests demonstrated the ability of the Streptomyces sp. myrophorea, isolate McG1 to inhibit the growth of many strains of ESKAPE pathogens; most notably carbapenem-resistant Acinetobacter baumannii (a critical pathogen on the WHO priority list of antibiotic-resistant bacteria), vancomycin-resistant Enterococcus faecium, and methicillin-resistant Staphylococcus aureus (both listed as high priority pathogens). Further in silico prediction of antimicrobial potential of Streptomyces sp. myrophorea, isolate McG1 by anti-SMASH and RAST software identified many secondary metabolite and toxicity resistance gene clusters (45 and 27, respectively) as well as many antibiotic resistance genes potentially related to antibiotic production. Follow-up in vitro tests show that the Streptomyces sp. myrophorea, isolate McG1 was resistant to 28 out of 36 clinical antibiotics. Although not a comprehensive analysis, we think that some of the Boho soils’ reputed curative properties may be linked to the ability of Streptomyces sp. myrophorea, isolate McG1 to inhibit ESKAPE pathogens. More importantly, further analysis may elucidate other key components that could alleviate the tide of multi-resistant nosocomial infections. Journal Article Frontiers in Microbiology 9 Frontiers Media SA 1664-302X 16 10 2018 2018-10-16 10.3389/fmicb.2018.02458 http://dx.doi.org/10.3389/fmicb.2018.02458 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2021-01-27T17:58:27.9727522 2018-10-18T11:31:06.2282011 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Luciana Terra 1 Paul J. Dyson 2 Matthew Hitchings 0000-0002-5527-4709 3 Liam Thomas 4 Alyaa Abdelhameed 5 Ibrahim M. Banat 6 Salvatore A. Gazze 7 Dušica Vujaklija 8 Paul Facey 0000-0002-3229-0255 9 Lewis Francis 0000-0002-7803-7714 10 Gerry A. Quinn 11 Paul Dyson 0000-0002-0558-2666 12 0044961-05112018102706.pdf 44961.pdf 2018-11-05T10:27:06.9030000 Output 2605691 application/pdf Version of Record true 2018-11-04T00:00:00.0000000 Released under the terms of the Creative Commons Attribution License (CC-BY). true eng
title	A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens
spellingShingle	A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens Matthew Hitchings Paul Facey Lewis Francis Paul Dyson
title_short	A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens
title_full	A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens
title_fullStr	A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens
title_full_unstemmed	A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens
title_sort	A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens
author_id_str_mv	be98847c72c14a731c4a6b7bc02b3bcf dc25910b8004b2694df68ed7426e1286 10f61f9c1248951c1a33f6a89498f37d 300e3f46b70ae83f563b24f41d00cd17
author_id_fullname_str_mv	be98847c72c14a731c4a6b7bc02b3bcf_*_Matthew Hitchings dc25910b8004b2694df68ed7426e1286__Paul Facey 10f61f9c1248951c1a33f6a89498f37d__Lewis Francis 300e3f46b70ae83f563b24f41d00cd17_*_Paul Dyson
author	Matthew Hitchings Paul Facey Lewis Francis Paul Dyson
author2	Luciana Terra Paul J. Dyson Matthew Hitchings Liam Thomas Alyaa Abdelhameed Ibrahim M. Banat Salvatore A. Gazze Dušica Vujaklija Paul Facey Lewis Francis Gerry A. Quinn Paul Dyson
format	Journal article
container_title	Frontiers in Microbiology
container_volume	9
publishDate	2018
institution	Swansea University
issn	1664-302X
doi_str_mv	10.3389/fmicb.2018.02458
publisher	Frontiers Media SA
college_str	Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url	http://dx.doi.org/10.3389/fmicb.2018.02458
document_store_str	1
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description	In an effort to stem the rising tide of multi-resistant bacteria, researchers have turned to niche environments in the hope of discovering new varieties of antibiotics. We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ireland) for the presence of Streptomyces, a well-known producer of antibiotics. From this soil we isolated a novel (closest relative 57% of genome relatedness) Streptomyces sp. capable of growth at high alkaline pH (10.5) and tolerant of gamma radiation to 4 kGy. Genomic sequencing identified many alkaline tolerance (antiporter/multi-resistance) genes compared to S. coelicolor M145 (at 3:1), hence we designated the strain Streptomyces sp. myrophorea, isolate McG1, from the Greek, myro (fragrance) and phorea (porter/carrier). In vitro tests demonstrated the ability of the Streptomyces sp. myrophorea, isolate McG1 to inhibit the growth of many strains of ESKAPE pathogens; most notably carbapenem-resistant Acinetobacter baumannii (a critical pathogen on the WHO priority list of antibiotic-resistant bacteria), vancomycin-resistant Enterococcus faecium, and methicillin-resistant Staphylococcus aureus (both listed as high priority pathogens). Further in silico prediction of antimicrobial potential of Streptomyces sp. myrophorea, isolate McG1 by anti-SMASH and RAST software identified many secondary metabolite and toxicity resistance gene clusters (45 and 27, respectively) as well as many antibiotic resistance genes potentially related to antibiotic production. Follow-up in vitro tests show that the Streptomyces sp. myrophorea, isolate McG1 was resistant to 28 out of 36 clinical antibiotics. Although not a comprehensive analysis, we think that some of the Boho soils’ reputed curative properties may be linked to the ability of Streptomyces sp. myrophorea, isolate McG1 to inhibit ESKAPE pathogens. More importantly, further analysis may elucidate other key components that could alleviate the tide of multi-resistant nosocomial infections.
published_date	2018-10-16T03:56:28Z
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score	10.999252

A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens

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