No Cover Image

Journal article 491 views 66 downloads

Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure

Bethan Kultschar, Ed Dudley, Steve Wilson, Carole Llewellyn

Metabolites, Volume: 9, Issue: 4, Start page: 74

Swansea University Author: Carole Llewellyn

  • 50025.pdf

    PDF | Version of Record

    Released under the terms of a Creative Commons Attribution License (CC-BY).

    Download (2.99MB)

Check full text

DOI (Published version): 10.3390/metabo9040074

Abstract

Cyanobacteria have many defence strategies to overcome harmful ultraviolet (UV) stress including the production of secondary metabolites. Metabolomics can be used to investigate this altered metabolism via targeted and untargeted techniques. In this study we assessed the changes in the intra- and ex...

Full description

Published in: Metabolites
ISSN: 2218-1989
Published: Basel, Switzerland MDPI 2019
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa50025
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2019-04-18T12:09:42Z
last_indexed 2019-07-24T15:34:28Z
id cronfa50025
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2019-07-24T10:33:12.3598381</datestamp><bib-version>v2</bib-version><id>50025</id><entry>2019-04-16</entry><title>Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure</title><swanseaauthors><author><sid>bcd94bda79ebf4c2c82d82dfb027a140</sid><firstname>Carole</firstname><surname>Llewellyn</surname><name>Carole Llewellyn</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2019-04-16</date><deptcode>FGSEN</deptcode><abstract>Cyanobacteria have many defence strategies to overcome harmful ultraviolet (UV) stress including the production of secondary metabolites. Metabolomics can be used to investigate this altered metabolism via targeted and untargeted techniques. In this study we assessed the changes in the intra- and extracellular low molecular weight metabolite levels of Chlorogloeopsis fritschii (C. fritschii) during 48 h of photosynthetically active radiation (PAR) supplemented with UV-B (15 &#xB5;mol m&#x2212;2 s&#x2212;1 of PAR plus 3 &#xB5;mol m&#x2212;2 s&#x2212;1 of UV-B) and intracellular levels during 48 h of PAR only (15 &#xB5;mol m&#x2212;2 s&#x2212;1) with sampling points at 0, 2, 6, 12, 24 and 48 h. Gas chromatography&#x2013;mass spectrometry (GC&#x2013;MS) was used as a metabolite profiling tool to investigate the global changes in metabolite levels. The UV-B time series experiment showed an overall significant reduction in intracellular metabolites involved with carbon and nitrogen metabolism such as the amino acids tyrosine and phenylalanine which have a role in secondary metabolite production. Significant accumulation of proline was observed with a potential role in stress mitigation as seen in other photosynthetic organisms. 12 commonly identified metabolites were measured in both UV-B exposed (PAR + UV-B) and PAR only experiments with differences in significance observed. Extracellular metabolites (PAR + UV-B) showed accumulation of sugars as seen in other cyanobacterial species as a stress response to UV-B. In conclusion, a snapshot of the metabolome of C. fritschii was measured. Little work has been undertaken on C. fritschii, a novel candidate for use in industrial biotechnology, with, to our knowledge, no previous literature on combined intra- and extracellular analysis during a UV-B treatment time-series. This study is important to build on experimental data already available for cyanobacteria and other photosynthetic organisms exposed to UV-B</abstract><type>Journal Article</type><journal>Metabolites</journal><volume>9</volume><journalNumber>4</journalNumber><paginationStart>74</paginationStart><publisher>MDPI</publisher><placeOfPublication>Basel, Switzerland</placeOfPublication><issnElectronic>2218-1989</issnElectronic><keywords>cyanobacteria; C. fritschii; UV-B; PAR; time-series; intracellular; extracellular; metabolites;GC-MS</keywords><publishedDay>16</publishedDay><publishedMonth>4</publishedMonth><publishedYear>2019</publishedYear><publishedDate>2019-04-16</publishedDate><doi>10.3390/metabo9040074</doi><url/><notes/><college>COLLEGE NANME</college><department>Science and Engineering - Faculty</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>FGSEN</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2019-07-24T10:33:12.3598381</lastEdited><Created>2019-04-16T12:59:06.0318250</Created><path><level id="1">College of Science</level><level id="2">Biosciences</level></path><authors><author><firstname>Bethan</firstname><surname>Kultschar</surname><order>1</order></author><author><firstname>Ed</firstname><surname>Dudley</surname><order>2</order></author><author><firstname>Steve</firstname><surname>Wilson</surname><order>3</order></author><author><firstname>Carole</firstname><surname>Llewellyn</surname><order>4</order></author></authors><documents><document><filename>0050025-15052019090440.pdf</filename><originalFilename>50025.pdf</originalFilename><uploaded>2019-05-15T09:04:40.0270000</uploaded><type>Output</type><contentLength>3083705</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2019-05-14T00:00:00.0000000</embargoDate><documentNotes>Released under the terms of a Creative Commons Attribution License (CC-BY).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2019-07-24T10:33:12.3598381 v2 50025 2019-04-16 Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure bcd94bda79ebf4c2c82d82dfb027a140 Carole Llewellyn Carole Llewellyn true false 2019-04-16 FGSEN Cyanobacteria have many defence strategies to overcome harmful ultraviolet (UV) stress including the production of secondary metabolites. Metabolomics can be used to investigate this altered metabolism via targeted and untargeted techniques. In this study we assessed the changes in the intra- and extracellular low molecular weight metabolite levels of Chlorogloeopsis fritschii (C. fritschii) during 48 h of photosynthetically active radiation (PAR) supplemented with UV-B (15 µmol m−2 s−1 of PAR plus 3 µmol m−2 s−1 of UV-B) and intracellular levels during 48 h of PAR only (15 µmol m−2 s−1) with sampling points at 0, 2, 6, 12, 24 and 48 h. Gas chromatography–mass spectrometry (GC–MS) was used as a metabolite profiling tool to investigate the global changes in metabolite levels. The UV-B time series experiment showed an overall significant reduction in intracellular metabolites involved with carbon and nitrogen metabolism such as the amino acids tyrosine and phenylalanine which have a role in secondary metabolite production. Significant accumulation of proline was observed with a potential role in stress mitigation as seen in other photosynthetic organisms. 12 commonly identified metabolites were measured in both UV-B exposed (PAR + UV-B) and PAR only experiments with differences in significance observed. Extracellular metabolites (PAR + UV-B) showed accumulation of sugars as seen in other cyanobacterial species as a stress response to UV-B. In conclusion, a snapshot of the metabolome of C. fritschii was measured. Little work has been undertaken on C. fritschii, a novel candidate for use in industrial biotechnology, with, to our knowledge, no previous literature on combined intra- and extracellular analysis during a UV-B treatment time-series. This study is important to build on experimental data already available for cyanobacteria and other photosynthetic organisms exposed to UV-B Journal Article Metabolites 9 4 74 MDPI Basel, Switzerland 2218-1989 cyanobacteria; C. fritschii; UV-B; PAR; time-series; intracellular; extracellular; metabolites;GC-MS 16 4 2019 2019-04-16 10.3390/metabo9040074 COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University 2019-07-24T10:33:12.3598381 2019-04-16T12:59:06.0318250 College of Science Biosciences Bethan Kultschar 1 Ed Dudley 2 Steve Wilson 3 Carole Llewellyn 4 0050025-15052019090440.pdf 50025.pdf 2019-05-15T09:04:40.0270000 Output 3083705 application/pdf Version of Record true 2019-05-14T00:00:00.0000000 Released under the terms of a Creative Commons Attribution License (CC-BY). true eng
title Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure
spellingShingle Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure
Carole Llewellyn
title_short Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure
title_full Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure
title_fullStr Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure
title_full_unstemmed Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure
title_sort Intracellular and Extracellular Metabolites from the Cyanobacterium Chlorogloeopsis fritschii, PCC 6912, During 48 Hours of UV-B Exposure
author_id_str_mv bcd94bda79ebf4c2c82d82dfb027a140
author_id_fullname_str_mv bcd94bda79ebf4c2c82d82dfb027a140_***_Carole Llewellyn
author Carole Llewellyn
author2 Bethan Kultschar
Ed Dudley
Steve Wilson
Carole Llewellyn
format Journal article
container_title Metabolites
container_volume 9
container_issue 4
container_start_page 74
publishDate 2019
institution Swansea University
issn 2218-1989
doi_str_mv 10.3390/metabo9040074
publisher MDPI
college_str College of Science
hierarchytype
hierarchy_top_id collegeofscience
hierarchy_top_title College of Science
hierarchy_parent_id collegeofscience
hierarchy_parent_title College of Science
department_str Biosciences{{{_:::_}}}College of Science{{{_:::_}}}Biosciences
document_store_str 1
active_str 0
description Cyanobacteria have many defence strategies to overcome harmful ultraviolet (UV) stress including the production of secondary metabolites. Metabolomics can be used to investigate this altered metabolism via targeted and untargeted techniques. In this study we assessed the changes in the intra- and extracellular low molecular weight metabolite levels of Chlorogloeopsis fritschii (C. fritschii) during 48 h of photosynthetically active radiation (PAR) supplemented with UV-B (15 µmol m−2 s−1 of PAR plus 3 µmol m−2 s−1 of UV-B) and intracellular levels during 48 h of PAR only (15 µmol m−2 s−1) with sampling points at 0, 2, 6, 12, 24 and 48 h. Gas chromatography–mass spectrometry (GC–MS) was used as a metabolite profiling tool to investigate the global changes in metabolite levels. The UV-B time series experiment showed an overall significant reduction in intracellular metabolites involved with carbon and nitrogen metabolism such as the amino acids tyrosine and phenylalanine which have a role in secondary metabolite production. Significant accumulation of proline was observed with a potential role in stress mitigation as seen in other photosynthetic organisms. 12 commonly identified metabolites were measured in both UV-B exposed (PAR + UV-B) and PAR only experiments with differences in significance observed. Extracellular metabolites (PAR + UV-B) showed accumulation of sugars as seen in other cyanobacterial species as a stress response to UV-B. In conclusion, a snapshot of the metabolome of C. fritschii was measured. Little work has been undertaken on C. fritschii, a novel candidate for use in industrial biotechnology, with, to our knowledge, no previous literature on combined intra- and extracellular analysis during a UV-B treatment time-series. This study is important to build on experimental data already available for cyanobacteria and other photosynthetic organisms exposed to UV-B
published_date 2019-04-16T04:04:07Z
_version_ 1736664833075445760
score 10.876655