Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells

Shivaji, Kavitha; Balasubramanian, Mythili Gnanamangai; Devadoss, Anitha; Asokan, Vijayshankar; De, Catherine Suenne; Davies, Matthew; Ponmurugan, Ponnusammy; Pitchaimuthu, Sudhagar

doi:10.1016/j.apsusc.2019.05.050

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Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells

Kavitha Shivaji, Mythili Gnanamangai Balasubramanian, Anitha Devadoss

, Vijayshankar Asokan, Catherine Suenne De Castro, Matthew Davies

, Ponnusammy Ponmurugan, Sudhagar Pitchaimuthu

Applied Surface Science

Swansea University Authors: Anitha Devadoss , Matthew Davies , Sudhagar Pitchaimuthu

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DOI (Published version): 10.1016/j.apsusc.2019.05.050

Abstract

Green technology for nanoparticles synthesis is considered to be of great significance in biomedical applications. Recently, low dimensional semiconductor cadmium sulfide (CdS) quantum dots (QDs) have raised great attention due to their optical properties and wide usage in biomedical studies. In our...

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Published in:	Applied Surface Science
ISSN:	0169-4332
Published:	2019
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa50285
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first_indexed	2019-05-09T20:01:26Z
last_indexed	2020-08-13T03:17:33Z
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Recently, low dimensional semiconductor cadmium sulfide (CdS) quantum dots (QDs) have raised great attention due to their optical properties and wide usage in biomedical studies. In our present work, we demonstrate a simple green synthesis route for CdS QDs production using waste matured tea leaves (mother leaf) as bio-surfactant that are a waste product of the tea leaf industry and not suitable for drinking. The structural and morphological analysis showed waste tea leaf derived CdS QDs range from 2.5 to 4 nm in particle size with a cubic crystalline structure. Interestingly, these CdS QDs exhibit strong florescence emission with maximum around 670 nm. We explored the cytotoxic effect of waste tea leaf mediated CdS QDs (MT-CdS QDs) in breast cancer cell lines and compared their viability with standard drug - cisplatin. Our experimental studies strongly suggest that MT-CdS QDs exhibits cytotoxic effect on breast cancer cells and their performance was compared with standard drug cisplatin. To further understand the role of MT-CdS QDs towards cytotoxicity, the fluorescence microscopy and flow cytometry analysis were carried out. The flow cytometry results reveal that MT-CdS QDs induces cell death as it arrests the cell cycle at S phase as well as G2/M phase. Further the apoptosis mechanism was confirmed with the expression of anti-apoptotic and apoptotic proteins. These studies explored that waste tea leaves have dual advantage – both in controlling the particle size of CdS QDs as well as facilitates their cytotoxicity effect in breast cancer cell death. Therefore, it is anticipated that the utilization of MT-CdS QDs produced from waste tea leaves as bi-functional drug and delivery vehicle in cancer treatment will be a promising approach. Also, this is a simple and circular economic route for producing biocompatible QDs at low-cost, which could simultaneously benefit tea and biomedical industries.</abstract><type>Journal Article</type><journal>Applied Surface Science</journal><publisher/><issnPrint>0169-4332</issnPrint><keywords>Quantum dots, CdS, Waste tea leaves, Green synthesis, Breast cancer, Apoptosis, Cytotoxicity</keywords><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2019</publishedYear><publishedDate>2019-12-31</publishedDate><doi>10.1016/j.apsusc.2019.05.050</doi><url/><notes/><college>COLLEGE NANME</college><department>Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>EEN</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2020-08-12T13:16:46.9780465</lastEdited><Created>2019-05-09T09:33:07.3250539</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Chemical Engineering</level></path><authors><author><firstname>Kavitha</firstname><surname>Shivaji</surname><order>1</order></author><author><firstname>Mythili Gnanamangai</firstname><surname>Balasubramanian</surname><order>2</order></author><author><firstname>Anitha</firstname><surname>Devadoss</surname><orcid>0000-0002-8052-1820</orcid><order>3</order></author><author><firstname>Vijayshankar</firstname><surname>Asokan</surname><order>4</order></author><author><firstname>Catherine Suenne De</firstname><surname>Castro</surname><order>5</order></author><author><firstname>Matthew</firstname><surname>Davies</surname><orcid>0000-0003-2595-5121</orcid><order>6</order></author><author><firstname>Ponnusammy</firstname><surname>Ponmurugan</surname><order>7</order></author><author><firstname>Sudhagar</firstname><surname>Pitchaimuthu</surname><orcid>0000-0001-9098-8806</orcid><order>8</order></author></authors><documents><document><filename>0050285-09052019093623.pdf</filename><originalFilename>shivaji2019v2.pdf</originalFilename><uploaded>2019-05-09T09:36:23.5070000</uploaded><type>Output</type><contentLength>11309724</contentLength><contentType>application/pdf</contentType><version>Accepted Manuscript</version><cronfaStatus>true</cronfaStatus><embargoDate>2020-05-07T00:00:00.0000000</embargoDate><documentNotes>Released with a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND)</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling	2020-08-12T13:16:46.9780465 v2 50285 2019-05-09 Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells a01150750f1c8eccbfeebffdde3fe8a1 0000-0002-8052-1820 Anitha Devadoss Anitha Devadoss true false 4ad478e342120ca3434657eb13527636 0000-0003-2595-5121 Matthew Davies Matthew Davies true false 2fdbee02f4bfc5a1b174c8bd04afbd2b 0000-0001-9098-8806 Sudhagar Pitchaimuthu Sudhagar Pitchaimuthu true false 2019-05-09 EEN Green technology for nanoparticles synthesis is considered to be of great significance in biomedical applications. Recently, low dimensional semiconductor cadmium sulfide (CdS) quantum dots (QDs) have raised great attention due to their optical properties and wide usage in biomedical studies. In our present work, we demonstrate a simple green synthesis route for CdS QDs production using waste matured tea leaves (mother leaf) as bio-surfactant that are a waste product of the tea leaf industry and not suitable for drinking. The structural and morphological analysis showed waste tea leaf derived CdS QDs range from 2.5 to 4 nm in particle size with a cubic crystalline structure. Interestingly, these CdS QDs exhibit strong florescence emission with maximum around 670 nm. We explored the cytotoxic effect of waste tea leaf mediated CdS QDs (MT-CdS QDs) in breast cancer cell lines and compared their viability with standard drug - cisplatin. Our experimental studies strongly suggest that MT-CdS QDs exhibits cytotoxic effect on breast cancer cells and their performance was compared with standard drug cisplatin. To further understand the role of MT-CdS QDs towards cytotoxicity, the fluorescence microscopy and flow cytometry analysis were carried out. The flow cytometry results reveal that MT-CdS QDs induces cell death as it arrests the cell cycle at S phase as well as G2/M phase. Further the apoptosis mechanism was confirmed with the expression of anti-apoptotic and apoptotic proteins. These studies explored that waste tea leaves have dual advantage – both in controlling the particle size of CdS QDs as well as facilitates their cytotoxicity effect in breast cancer cell death. Therefore, it is anticipated that the utilization of MT-CdS QDs produced from waste tea leaves as bi-functional drug and delivery vehicle in cancer treatment will be a promising approach. Also, this is a simple and circular economic route for producing biocompatible QDs at low-cost, which could simultaneously benefit tea and biomedical industries. Journal Article Applied Surface Science 0169-4332 Quantum dots, CdS, Waste tea leaves, Green synthesis, Breast cancer, Apoptosis, Cytotoxicity 31 12 2019 2019-12-31 10.1016/j.apsusc.2019.05.050 COLLEGE NANME Engineering COLLEGE CODE EEN Swansea University 2020-08-12T13:16:46.9780465 2019-05-09T09:33:07.3250539 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Kavitha Shivaji 1 Mythili Gnanamangai Balasubramanian 2 Anitha Devadoss 0000-0002-8052-1820 3 Vijayshankar Asokan 4 Catherine Suenne De Castro 5 Matthew Davies 0000-0003-2595-5121 6 Ponnusammy Ponmurugan 7 Sudhagar Pitchaimuthu 0000-0001-9098-8806 8 0050285-09052019093623.pdf shivaji2019v2.pdf 2019-05-09T09:36:23.5070000 Output 11309724 application/pdf Accepted Manuscript true 2020-05-07T00:00:00.0000000 Released with a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND) true eng
title	Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells
spellingShingle	Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells Anitha Devadoss Matthew Davies Sudhagar Pitchaimuthu
title_short	Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells
title_full	Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells
title_fullStr	Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells
title_full_unstemmed	Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells
title_sort	Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells
author_id_str_mv	a01150750f1c8eccbfeebffdde3fe8a1 4ad478e342120ca3434657eb13527636 2fdbee02f4bfc5a1b174c8bd04afbd2b
author_id_fullname_str_mv	a01150750f1c8eccbfeebffdde3fe8a1_*_Anitha Devadoss 4ad478e342120ca3434657eb13527636__Matthew Davies 2fdbee02f4bfc5a1b174c8bd04afbd2b_**_Sudhagar Pitchaimuthu
author	Anitha Devadoss Matthew Davies Sudhagar Pitchaimuthu
author2	Kavitha Shivaji Mythili Gnanamangai Balasubramanian Anitha Devadoss Vijayshankar Asokan Catherine Suenne De Castro Matthew Davies Ponnusammy Ponmurugan Sudhagar Pitchaimuthu
format	Journal article
container_title	Applied Surface Science
publishDate	2019
institution	Swansea University
issn	0169-4332
doi_str_mv	10.1016/j.apsusc.2019.05.050
college_str	Faculty of Science and Engineering
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department_str	School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
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description	Green technology for nanoparticles synthesis is considered to be of great significance in biomedical applications. Recently, low dimensional semiconductor cadmium sulfide (CdS) quantum dots (QDs) have raised great attention due to their optical properties and wide usage in biomedical studies. In our present work, we demonstrate a simple green synthesis route for CdS QDs production using waste matured tea leaves (mother leaf) as bio-surfactant that are a waste product of the tea leaf industry and not suitable for drinking. The structural and morphological analysis showed waste tea leaf derived CdS QDs range from 2.5 to 4 nm in particle size with a cubic crystalline structure. Interestingly, these CdS QDs exhibit strong florescence emission with maximum around 670 nm. We explored the cytotoxic effect of waste tea leaf mediated CdS QDs (MT-CdS QDs) in breast cancer cell lines and compared their viability with standard drug - cisplatin. Our experimental studies strongly suggest that MT-CdS QDs exhibits cytotoxic effect on breast cancer cells and their performance was compared with standard drug cisplatin. To further understand the role of MT-CdS QDs towards cytotoxicity, the fluorescence microscopy and flow cytometry analysis were carried out. The flow cytometry results reveal that MT-CdS QDs induces cell death as it arrests the cell cycle at S phase as well as G2/M phase. Further the apoptosis mechanism was confirmed with the expression of anti-apoptotic and apoptotic proteins. These studies explored that waste tea leaves have dual advantage – both in controlling the particle size of CdS QDs as well as facilitates their cytotoxicity effect in breast cancer cell death. Therefore, it is anticipated that the utilization of MT-CdS QDs produced from waste tea leaves as bi-functional drug and delivery vehicle in cancer treatment will be a promising approach. Also, this is a simple and circular economic route for producing biocompatible QDs at low-cost, which could simultaneously benefit tea and biomedical industries.
published_date	2019-12-31T04:01:41Z
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Utilization of waste tea leaves as bio-surfactant in CdS quantum dots synthesis and their cytotoxicity effect in breast cancer cells

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