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Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity

Emily Preedy Orcid Logo, Stefano Perni, Polina Prokopovich

Colloids and Surfaces B: Biointerfaces, Volume: 157, Pages: 146 - 156

Swansea University Author: Emily Preedy Orcid Logo

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DOI (Published version): 10.1016/j.colsurfb.2017.05.019

Abstract

Bone cells are damaged by wear particles originating from total joint replacement implants. We investigated Mesenchymal stem cells (MSCs) nanomechanical properties when exposed to cobalt and titanium nanoparticles (resembling wear debris) of different sizes for up to 3 days using AFM nanoindentation...

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Published in: Colloids and Surfaces B: Biointerfaces
ISSN: 0927-7765
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa51204
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Abstract: Bone cells are damaged by wear particles originating from total joint replacement implants. We investigated Mesenchymal stem cells (MSCs) nanomechanical properties when exposed to cobalt and titanium nanoparticles (resembling wear debris) of different sizes for up to 3 days using AFM nanoindentation; along with flow-cytometry and MTT assay. The results demonstrated that cells exposed to increasing concentrations of nanoparticles had a lower value of elasticity and spring constant without significant effect on cell metabolic activity and viability but some morphological alteration (bleeping). Cobalt induced greater effects than titanium and this is consistent with the general knowledge of cyto-compatibility of the later.This work demonstrates for the first time that metal nanoparticles do not only influence MSCs enzymes activity but also cell structure; however, they do not result in full membrane damage. Furthermore, the mechanical changes are concentration and particles composition dependent but little influenced by the particle size.
Keywords: Mesenchimal stem cells, Wear particles, Nanomechanical properties, AFM, Titanium nanoparticles, Cobalt nanoparticles
College: Faculty of Science and Engineering
Start Page: 146
End Page: 156

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