Journal article 193 views
Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli
Langmuir, Volume: 32, Issue: 31, Pages: 7965 - 7974
Swansea University Author: Emily Preedy
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DOI (Published version): 10.1021/acs.langmuir.6b02342
Abstract
Curli are bacterial appendages involved in the adhesion of cells to surfaces; their synthesis is regulated by many genes such as csgD and ompR. The expression of the two curli subunits (CsgA and CsgB) in Escherichia coli (E. coli) is regulated by CsgD; at the same time, csgD transcription is under t...
Published in: | Langmuir |
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ISSN: | 0743-7463 1520-5827 |
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2016
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URI: | https://cronfa.swan.ac.uk/Record/cronfa51206 |
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2019-08-07T12:33:57.2817826 v2 51206 2019-07-25 Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli a4ae2d29d6b017b303c85efa3a9503d0 0000-0003-0377-6747 Emily Preedy Emily Preedy true false 2019-07-25 CHEG Curli are bacterial appendages involved in the adhesion of cells to surfaces; their synthesis is regulated by many genes such as csgD and ompR. The expression of the two curli subunits (CsgA and CsgB) in Escherichia coli (E. coli) is regulated by CsgD; at the same time, csgD transcription is under the control of OmpR. Therefore, both genes are involved in the control of curli production. In this work, we elucidated the role of these genes in the nanomechanical and adhesive properties of E. coli MG1655 (a laboratory strain not expressing significant amount of curli) and its curli-producing mutants overexpressing OmpR and CsgD, employing atomic force microscopy (AFM). Nanomechanical analysis revealed that the expression of these genes gave origin to cells with a lower Young’s modulus (E) and turgidity (P0), whereas the adhesion forces were unaffected when genes involved in curli formation were expressed. AFM was also employed to study the primary structure of the curli expressed through the freely jointed chain (FJC) model for polymers. CsgD increased the number of curli on the surface more than OmpR did, and the overexpression of both genes did not result in a greater number of curli. Neither of the genes had an impact on the structure (total length of the polymer and number and length of Kuhn segments) of the curli. Our results further suggest that, despite the widely assumed role of curli in cell adhesion, cell adhesion force is also dictated by surface properties because no relation between the number of curli expressed on the surface and cell adhesion was found. Journal Article Langmuir 32 31 7965 7974 0743-7463 1520-5827 9 8 2016 2016-08-09 10.1021/acs.langmuir.6b02342 COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University 2019-08-07T12:33:57.2817826 2019-07-25T14:14:38.7197375 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Stefano Perni 1 Emily Preedy 0000-0003-0377-6747 2 Paolo Landini 3 Polina Prokopovich 4 |
title |
Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli |
spellingShingle |
Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli Emily Preedy |
title_short |
Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli |
title_full |
Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli |
title_fullStr |
Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli |
title_full_unstemmed |
Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli |
title_sort |
Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli |
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a4ae2d29d6b017b303c85efa3a9503d0 |
author_id_fullname_str_mv |
a4ae2d29d6b017b303c85efa3a9503d0_***_Emily Preedy |
author |
Emily Preedy |
author2 |
Stefano Perni Emily Preedy Paolo Landini Polina Prokopovich |
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Journal article |
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Langmuir |
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32 |
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31 |
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7965 |
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2016 |
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Swansea University |
issn |
0743-7463 1520-5827 |
doi_str_mv |
10.1021/acs.langmuir.6b02342 |
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Faculty of Science and Engineering |
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Faculty of Science and Engineering |
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Faculty of Science and Engineering |
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School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering |
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description |
Curli are bacterial appendages involved in the adhesion of cells to surfaces; their synthesis is regulated by many genes such as csgD and ompR. The expression of the two curli subunits (CsgA and CsgB) in Escherichia coli (E. coli) is regulated by CsgD; at the same time, csgD transcription is under the control of OmpR. Therefore, both genes are involved in the control of curli production. In this work, we elucidated the role of these genes in the nanomechanical and adhesive properties of E. coli MG1655 (a laboratory strain not expressing significant amount of curli) and its curli-producing mutants overexpressing OmpR and CsgD, employing atomic force microscopy (AFM). Nanomechanical analysis revealed that the expression of these genes gave origin to cells with a lower Young’s modulus (E) and turgidity (P0), whereas the adhesion forces were unaffected when genes involved in curli formation were expressed. AFM was also employed to study the primary structure of the curli expressed through the freely jointed chain (FJC) model for polymers. CsgD increased the number of curli on the surface more than OmpR did, and the overexpression of both genes did not result in a greater number of curli. Neither of the genes had an impact on the structure (total length of the polymer and number and length of Kuhn segments) of the curli. Our results further suggest that, despite the widely assumed role of curli in cell adhesion, cell adhesion force is also dictated by surface properties because no relation between the number of curli expressed on the surface and cell adhesion was found. |
published_date |
2016-08-09T04:03:00Z |
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1763753256070152192 |
score |
11.02167 |