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Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization

Alexander F. Mason, N. Amy Yewdall, Pascal L. W. Welzen, Jingxin Shao, Marleen van Stevendaal, Jan C. M. van Hest, David Williams Orcid Logo, Loai K. E. A. Abdelmohsen

ACS Central Science, Volume: 5, Issue: 8, Pages: 1360 - 1365

Swansea University Author: David Williams Orcid Logo

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DOI (Published version): 10.1021/acscentsci.9b00345

Abstract

A systemic feature of eukaryotic cells is the spatial organization of functional components through compartmentalization. Developing protocells with compartmentalized synthetic organelles is, therefore, a critical milestone toward emulating one of the core characteristics of cellular life. Here we d...

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Published in: ACS Central Science
ISSN: 2374-7943 2374-7951
Published: American Chemical Society (ACS) 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa51340
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first_indexed 2019-08-09T16:32:05Z
last_indexed 2019-08-16T15:30:13Z
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spelling 2019-08-16T13:11:05.8763554 v2 51340 2019-08-07 Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization c9047e773a645b7cb459ac4beedd0a37 0000-0002-8209-6899 David Williams David Williams true false 2019-08-07 SCH A systemic feature of eukaryotic cells is the spatial organization of functional components through compartmentalization. Developing protocells with compartmentalized synthetic organelles is, therefore, a critical milestone toward emulating one of the core characteristics of cellular life. Here we demonstrate the bottom-up, multistep, noncovalent, assembly of rudimentary subcompartmentalized protocells through the spontaneous encapsulation of semipermeable, polymersome proto-organelles inside cell-sized coacervates. The coacervate microdroplets are membranized using tailor-made terpolymers, to complete the hierarchical self-assembly of protocells, a system that mimics both the condensed cytosol and the structure of a cell membrane. In this way, the spatial organization of enzymes can be finely tuned, leading to an enhancement of functionality. Moreover, incompatible components can be sequestered in the same microenvironments without detrimental effect. The robust stability of the subcompartmentalized coacervate protocells in biocompatible milieu, such as in PBS or cell culture media, makes it a versatile platform to be extended toward studies in vitro, and perhaps, in vivo. Journal Article ACS Central Science 5 8 1360 1365 American Chemical Society (ACS) 2374-7943 2374-7951 28 8 2019 2019-08-28 10.1021/acscentsci.9b00345 COLLEGE NANME Chemistry COLLEGE CODE SCH Swansea University 2019-08-16T13:11:05.8763554 2019-08-07T11:35:20.0195360 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemistry Alexander F. Mason 1 N. Amy Yewdall 2 Pascal L. W. Welzen 3 Jingxin Shao 4 Marleen van Stevendaal 5 Jan C. M. van Hest 6 David Williams 0000-0002-8209-6899 7 Loai K. E. A. Abdelmohsen 8 51340__15920__94596c8046f941a99b47f6ff70dd8ba9.pdf 51340.pdf 2019-11-20T15:26:57.5153769 Output 5111919 application/pdf Version of Record true Released under the terms of a Creative Commons Attribution Non-Commercial No Derivative License (CC-BY-NC-ND). true https://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html
title Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization
spellingShingle Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization
David Williams
title_short Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization
title_full Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization
title_fullStr Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization
title_full_unstemmed Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization
title_sort Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization
author_id_str_mv c9047e773a645b7cb459ac4beedd0a37
author_id_fullname_str_mv c9047e773a645b7cb459ac4beedd0a37_***_David Williams
author David Williams
author2 Alexander F. Mason
N. Amy Yewdall
Pascal L. W. Welzen
Jingxin Shao
Marleen van Stevendaal
Jan C. M. van Hest
David Williams
Loai K. E. A. Abdelmohsen
format Journal article
container_title ACS Central Science
container_volume 5
container_issue 8
container_start_page 1360
publishDate 2019
institution Swansea University
issn 2374-7943
2374-7951
doi_str_mv 10.1021/acscentsci.9b00345
publisher American Chemical Society (ACS)
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemistry{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemistry
document_store_str 1
active_str 0
description A systemic feature of eukaryotic cells is the spatial organization of functional components through compartmentalization. Developing protocells with compartmentalized synthetic organelles is, therefore, a critical milestone toward emulating one of the core characteristics of cellular life. Here we demonstrate the bottom-up, multistep, noncovalent, assembly of rudimentary subcompartmentalized protocells through the spontaneous encapsulation of semipermeable, polymersome proto-organelles inside cell-sized coacervates. The coacervate microdroplets are membranized using tailor-made terpolymers, to complete the hierarchical self-assembly of protocells, a system that mimics both the condensed cytosol and the structure of a cell membrane. In this way, the spatial organization of enzymes can be finely tuned, leading to an enhancement of functionality. Moreover, incompatible components can be sequestered in the same microenvironments without detrimental effect. The robust stability of the subcompartmentalized coacervate protocells in biocompatible milieu, such as in PBS or cell culture media, makes it a versatile platform to be extended toward studies in vitro, and perhaps, in vivo.
published_date 2019-08-28T04:03:11Z
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score 11.036531

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