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Effect of increased endogenous glucose levels within Type 2 diabetes on cellular lipid profiles

Rachel Churm Orcid Logo, John Barry, Scott Caplin, Jeffrey Davies Orcid Logo, Jeffrey Stephens Orcid Logo, Sarah Prior Orcid Logo

Endocrine Abstracts, Volume: 48, Issue: 2

Swansea University Authors: Rachel Churm Orcid Logo, Jeffrey Davies Orcid Logo, Jeffrey Stephens Orcid Logo, Sarah Prior Orcid Logo

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DOI (Published version): 10.1530/endoabs.48.p2

Abstract

Background & Aims: ATP binding cassette subfamily G1 (ABCG1) is involved in mediating cholesterol efflux and modulating cellular lipid homeostasis. It is influenced by endogenous glucose and our aim was to examine the relationship between ABCG1 expression and lipid profiles within the increased...

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Published in: Endocrine Abstracts
ISSN: 1479-6848
Published: Bioscientifica 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa51491
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spelling 2019-09-04T11:23:32.2621566 v2 51491 2019-08-21 Effect of increased endogenous glucose levels within Type 2 diabetes on cellular lipid profiles c6cd8267ff0b13f2ea333bbfefdae144 0000-0001-9855-6282 Rachel Churm Rachel Churm true false 2cb3d1d96a7870a84d2f758e865172e6 0000-0002-4234-0033 Jeffrey Davies Jeffrey Davies true false 5219d126f97f8f884bdb622099bd41de 0000-0003-2228-086X Jeffrey Stephens Jeffrey Stephens true false cdda101035997acfaa6fdf17097f52b2 0000-0001-8703-8092 Sarah Prior Sarah Prior true false 2019-08-21 STSC Background & Aims: ATP binding cassette subfamily G1 (ABCG1) is involved in mediating cholesterol efflux and modulating cellular lipid homeostasis. It is influenced by endogenous glucose and our aim was to examine the relationship between ABCG1 expression and lipid profiles within the increased glucose levels of subjects with Type 2 diabetes (ODM).Methods: RNA was extracted from visceral fat collected from subjects undergoing abdominal surgery (bariatric and routine non-acute, non-malignant conditions). 30 subjects were categorised as non-obese (NO, n=10), obese (O, n=10) or ODM (n=10). Samples were examined using Real-Time qPCR and 2−ΔΔCt data analysis in order to determine gene expression changes. Lipid profiles were measured using a colorimetric assay on the Randox Daytona Plus™.Results & Conclusions: All three subject groups were well matched with weight/BMI being the only significant differences between clinical parameters. There was no overall change in the expression of ABCG1 when comparing the O to the NO group. Reduced expression of ABCG1 was observed in ODM compared to O and the NO group (ABCG1=2.0-fold and 2.4- fold decrease, respectively). An increase in plasma glucose correlate with the reduced expression of ABCG1 (P<0.05). Data suggests that this is having a knock on effect on lipid profile markers. Within the ODM there is a significant decrease in plasma levels of total cholesterol, HDL and LDLs (P; <0.05, <0.05, <0.01 respectively). This decrease in plasma lipids is independent of prescribed statin use. Therefore, hypothesised to be the result of a reduction in cholesterol export from the cell and consequently cellular lipid retention. Journal Article Endocrine Abstracts 48 2 Bioscientifica 1479-6848 16 3 2017 2017-03-16 10.1530/endoabs.48.p2 COLLEGE NANME Sport and Exercise Sciences COLLEGE CODE STSC Swansea University 2019-09-04T11:23:32.2621566 2019-08-21T10:19:34.0353540 College of Engineering Sports Science Rachel Churm 0000-0001-9855-6282 1 John Barry 2 Scott Caplin 3 Jeffrey Davies 0000-0002-4234-0033 4 Jeffrey Stephens 0000-0003-2228-086X 5 Sarah Prior 0000-0001-8703-8092 6
title Effect of increased endogenous glucose levels within Type 2 diabetes on cellular lipid profiles
spellingShingle Effect of increased endogenous glucose levels within Type 2 diabetes on cellular lipid profiles
Rachel Churm
Jeffrey Davies
Jeffrey Stephens
Sarah Prior
title_short Effect of increased endogenous glucose levels within Type 2 diabetes on cellular lipid profiles
title_full Effect of increased endogenous glucose levels within Type 2 diabetes on cellular lipid profiles
title_fullStr Effect of increased endogenous glucose levels within Type 2 diabetes on cellular lipid profiles
title_full_unstemmed Effect of increased endogenous glucose levels within Type 2 diabetes on cellular lipid profiles
title_sort Effect of increased endogenous glucose levels within Type 2 diabetes on cellular lipid profiles
author_id_str_mv c6cd8267ff0b13f2ea333bbfefdae144
2cb3d1d96a7870a84d2f758e865172e6
5219d126f97f8f884bdb622099bd41de
cdda101035997acfaa6fdf17097f52b2
author_id_fullname_str_mv c6cd8267ff0b13f2ea333bbfefdae144_***_Rachel Churm
2cb3d1d96a7870a84d2f758e865172e6_***_Jeffrey Davies
5219d126f97f8f884bdb622099bd41de_***_Jeffrey Stephens
cdda101035997acfaa6fdf17097f52b2_***_Sarah Prior
author Rachel Churm
Jeffrey Davies
Jeffrey Stephens
Sarah Prior
author2 Rachel Churm
John Barry
Scott Caplin
Jeffrey Davies
Jeffrey Stephens
Sarah Prior
format Journal article
container_title Endocrine Abstracts
container_volume 48
container_issue 2
publishDate 2017
institution Swansea University
issn 1479-6848
doi_str_mv 10.1530/endoabs.48.p2
publisher Bioscientifica
college_str College of Engineering
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hierarchy_top_id collegeofengineering
hierarchy_top_title College of Engineering
hierarchy_parent_id collegeofengineering
hierarchy_parent_title College of Engineering
department_str Sports Science{{{_:::_}}}College of Engineering{{{_:::_}}}Sports Science
document_store_str 0
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description Background & Aims: ATP binding cassette subfamily G1 (ABCG1) is involved in mediating cholesterol efflux and modulating cellular lipid homeostasis. It is influenced by endogenous glucose and our aim was to examine the relationship between ABCG1 expression and lipid profiles within the increased glucose levels of subjects with Type 2 diabetes (ODM).Methods: RNA was extracted from visceral fat collected from subjects undergoing abdominal surgery (bariatric and routine non-acute, non-malignant conditions). 30 subjects were categorised as non-obese (NO, n=10), obese (O, n=10) or ODM (n=10). Samples were examined using Real-Time qPCR and 2−ΔΔCt data analysis in order to determine gene expression changes. Lipid profiles were measured using a colorimetric assay on the Randox Daytona Plus™.Results & Conclusions: All three subject groups were well matched with weight/BMI being the only significant differences between clinical parameters. There was no overall change in the expression of ABCG1 when comparing the O to the NO group. Reduced expression of ABCG1 was observed in ODM compared to O and the NO group (ABCG1=2.0-fold and 2.4- fold decrease, respectively). An increase in plasma glucose correlate with the reduced expression of ABCG1 (P<0.05). Data suggests that this is having a knock on effect on lipid profile markers. Within the ODM there is a significant decrease in plasma levels of total cholesterol, HDL and LDLs (P; <0.05, <0.05, <0.01 respectively). This decrease in plasma lipids is independent of prescribed statin use. Therefore, hypothesised to be the result of a reduction in cholesterol export from the cell and consequently cellular lipid retention.
published_date 2017-03-16T04:05:07Z
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