Journal article 849 views
The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells
,
Joni Leivo,
Sari Vanhatupa,
Joose Kreutzer,
Ted J. Vaughan,
Anna-Maija Honkala,
Marlitt Viehrig,
Conleth A. Mullen,
Pasi Kallio,
Laoise M. McNamara,
Susanna Miettinen
Journal of the Mechanical Behavior of Biomedical Materials, Volume: 72, Pages: 38 - 48
Swansea University Author:
Feihu Zhao
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1016/j.jmbbm.2017.04.016
Abstract
Although mechanical cues are known to affect stem cell fate and mechanobiology, the significance of such stimuli on the osteogenic differentiation of human adipose stem cells (hASCs) remains unclear. In this study, we investigated the effect of long-term mechanical stimulation on the attachment, ost...
| Published in: | Journal of the Mechanical Behavior of Biomedical Materials |
|---|---|
| ISSN: | 1751-6161 |
| Published: |
2017
|
| Online Access: |
Check full text
|
| URI: | https://cronfa.swan.ac.uk/Record/cronfa51687 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract: |
Although mechanical cues are known to affect stem cell fate and mechanobiology, the significance of such stimuli on the osteogenic differentiation of human adipose stem cells (hASCs) remains unclear. In this study, we investigated the effect of long-term mechanical stimulation on the attachment, osteogenic differentiation and mechanical properties of hASCs. Tailor-made, pneumatic cell stretching devices were used to expose hASCs to cyclic equiaxial stretching in osteogenic medium. Cell attachment and focal adhesions were visualised using immunocytochemical vinculin staining on days 3 and 6, and the proliferation and alkaline phosphatase activity, as a sign of early osteogenic differentiation, were analysed on days 0, 6 and 10. Furthermore, the mechanical properties of hASCs, in terms of apparent Young’s modulus and normalised contractility, were obtained using a combination of atomic force microscopy based indentation and computational approaches. Our results indicated that cyclic equiaxial stretching delayed proliferation and promoted osteogenic differentiation of hASCs. Stretching also reduced cell size and intensified focal adhesions and actin cytoskeleton. Moreover, cell stiffening was observed during osteogenic differentiation and especially under mechanical stimulation. These results suggest that cyclic equiaxial stretching modifies cell morphology, focal adhesion formation and mechanical properties of hASCs. This could be exploited to enhance osteogenic differentiation. |
|---|---|
| Keywords: |
Mesenchymal stem cell, Dynamic cell culture, Mechanical stimulation, Atomic force microscopy indentation, PDMS, mechanobiology |
| College: |
Faculty of Science and Engineering |
| Start Page: |
38 |
| End Page: |
48 |