Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.

Davies, Charlotte; Bass, David; Ward, Georgia M.; Batista, Frederico; Malkin, Sophie; Thomas, Jessica; Bateman, Kelly; Feist, Stephen W.; Coates, Christopher; Rowley, Andrew

doi:10.1017/s0031182020000980

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Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.

Charlotte Davies

, David Bass, Georgia M. Ward, Frederico Batista, Sophie Malkin, Jessica Thomas, Kelly Bateman, Stephen W. Feist, Christopher Coates, Andrew Rowley

Parasitology, Volume: 147, Issue: 11, Pages: 1229 - 1237

Swansea University Authors: Charlotte Davies , Frederico Batista, Sophie Malkin, Jessica Thomas, Christopher Coates, Andrew Rowley

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DOI (Published version): 10.1017/s0031182020000980

Abstract

This study provides a morphological and phylogenetic characterization of two novel species of the order Haplosporida (Haplosporidium carcini n. sp., and H. cranc n. sp.) infecting the common shore crab Carcinus maenas collected at one location in Swansea Bay, South Wales, UK. Both parasites were obs...

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Published in:	Parasitology
ISSN:	0031-1820 1469-8161
Published:	Cambridge University Press (CUP) 2020
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URI:	https://cronfa.swan.ac.uk/Record/cronfa54427
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first_indexed	2020-06-10T19:08:16Z
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Both parasites were observed in the haemolymph, gills and hepatopancreas. The prevalence of clinical infections (i.e. parasites seen directly in fresh haemolymph preparations) was low, at ~1%, whereas subclinical levels, detected by polymerase chain reaction, were slightly higher at ~2%. Although no spores were found in any of the infected crabs examined histologically (n = 334), the morphology of monokaryotic and dikaryotic unicellular stages of the parasites enabled differentiation between the two new species. Phylogenetic analyses of the new species based on the small subunit (SSU) rDNA gene placed H. cranc in a clade of otherwise uncharacterized environmental sequences from marine samples, and H. carcini in a clade with other crustacean-associated lineages.</abstract><type>Journal Article</type><journal>Parasitology</journal><volume>147</volume><journalNumber>11</journalNumber><paginationStart>1229</paginationStart><paginationEnd>1237</paginationEnd><publisher>Cambridge University Press (CUP)</publisher><issnPrint>0031-1820</issnPrint><issnElectronic>1469-8161</issnElectronic><keywords>Ascetosporea, Carcinus maenas, diseas,e connectivity, Haplosporida, Haplosporidium carcini, Haplosporidium cranc, new species, parasite, phylogeny</keywords><publishedDay>1</publishedDay><publishedMonth>9</publishedMonth><publishedYear>2020</publishedYear><publishedDate>2020-09-01</publishedDate><doi>10.1017/s0031182020000980</doi><url/><notes/><college>COLLEGE NANME</college><department>Biosciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>SBI</DepartmentCode><institution>Swansea University</institution><apcterm/><funders>UKRI, BB/P017215/1</funders><lastEdited>2020-10-25T14:28:15.0577812</lastEdited><Created>2020-06-10T14:38:26.6551098</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Biosciences, Geography and Physics - Biosciences</level></path><authors><author><firstname>Charlotte</firstname><surname>Davies</surname><orcid>0000-0002-5853-1934</orcid><order>1</order></author><author><firstname>David</firstname><surname>Bass</surname><order>2</order></author><author><firstname>Georgia M.</firstname><surname>Ward</surname><order>3</order></author><author><firstname>Frederico</firstname><surname>Batista</surname><order>4</order></author><author><firstname>Sophie</firstname><surname>Malkin</surname><order>5</order></author><author><firstname>Jessica</firstname><surname>Thomas</surname><order>6</order></author><author><firstname>Kelly</firstname><surname>Bateman</surname><order>7</order></author><author><firstname>Stephen W.</firstname><surname>Feist</surname><order>8</order></author><author><firstname>Christopher</firstname><surname>Coates</surname><order>9</order></author><author><firstname>Andrew</firstname><surname>Rowley</surname><order>10</order></author></authors><documents><document><filename>54427__17751__a630330b2a034400a45921fcb30683ad.pdf</filename><originalFilename>54427.pdf</originalFilename><uploaded>2020-07-22T14:29:48.7408033</uploaded><type>Output</type><contentLength>1037716</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>The Author(s) 2020. 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spelling	2020-10-25T14:28:15.0577812 v2 54427 2020-06-10 Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp. a0febe211e502356dad1dab51a43761c 0000-0002-5853-1934 Charlotte Davies Charlotte Davies true false 77657489849b520529e56265f39a5724 Frederico Batista Frederico Batista true false 17ff4136248e9382802ced1bc1d680e4 Sophie Malkin Sophie Malkin true false 7989695d9a7bf8989a23e253204ac50b Jessica Thomas Jessica Thomas true false af160934b75bea5b8ba83d68b3d1a003 Christopher Coates Christopher Coates true false e98124f6e62b9592786899d7059e3a79 Andrew Rowley Andrew Rowley true false 2020-06-10 SBI This study provides a morphological and phylogenetic characterization of two novel species of the order Haplosporida (Haplosporidium carcini n. sp., and H. cranc n. sp.) infecting the common shore crab Carcinus maenas collected at one location in Swansea Bay, South Wales, UK. Both parasites were observed in the haemolymph, gills and hepatopancreas. The prevalence of clinical infections (i.e. parasites seen directly in fresh haemolymph preparations) was low, at ~1%, whereas subclinical levels, detected by polymerase chain reaction, were slightly higher at ~2%. Although no spores were found in any of the infected crabs examined histologically (n = 334), the morphology of monokaryotic and dikaryotic unicellular stages of the parasites enabled differentiation between the two new species. Phylogenetic analyses of the new species based on the small subunit (SSU) rDNA gene placed H. cranc in a clade of otherwise uncharacterized environmental sequences from marine samples, and H. carcini in a clade with other crustacean-associated lineages. Journal Article Parasitology 147 11 1229 1237 Cambridge University Press (CUP) 0031-1820 1469-8161 Ascetosporea, Carcinus maenas, diseas,e connectivity, Haplosporida, Haplosporidium carcini, Haplosporidium cranc, new species, parasite, phylogeny 1 9 2020 2020-09-01 10.1017/s0031182020000980 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University UKRI, BB/P017215/1 2020-10-25T14:28:15.0577812 2020-06-10T14:38:26.6551098 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Charlotte Davies 0000-0002-5853-1934 1 David Bass 2 Georgia M. Ward 3 Frederico Batista 4 Sophie Malkin 5 Jessica Thomas 6 Kelly Bateman 7 Stephen W. Feist 8 Christopher Coates 9 Andrew Rowley 10 54427__17751__a630330b2a034400a45921fcb30683ad.pdf 54427.pdf 2020-07-22T14:29:48.7408033 Output 1037716 application/pdf Version of Record true The Author(s) 2020. Published by Cambridge University Press. This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence. true English
title	Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
spellingShingle	Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp. Charlotte Davies Frederico Batista Sophie Malkin Jessica Thomas Christopher Coates Andrew Rowley
title_short	Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title_full	Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title_fullStr	Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title_full_unstemmed	Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title_sort	Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
author_id_str_mv	a0febe211e502356dad1dab51a43761c 77657489849b520529e56265f39a5724 17ff4136248e9382802ced1bc1d680e4 7989695d9a7bf8989a23e253204ac50b af160934b75bea5b8ba83d68b3d1a003 e98124f6e62b9592786899d7059e3a79
author_id_fullname_str_mv	a0febe211e502356dad1dab51a43761c_*_Charlotte Davies 77657489849b520529e56265f39a5724__Frederico Batista 17ff4136248e9382802ced1bc1d680e4__Sophie Malkin 7989695d9a7bf8989a23e253204ac50b__Jessica Thomas af160934b75bea5b8ba83d68b3d1a003__Christopher Coates e98124f6e62b9592786899d7059e3a79_*_Andrew Rowley
author	Charlotte Davies Frederico Batista Sophie Malkin Jessica Thomas Christopher Coates Andrew Rowley
author2	Charlotte Davies David Bass Georgia M. Ward Frederico Batista Sophie Malkin Jessica Thomas Kelly Bateman Stephen W. Feist Christopher Coates Andrew Rowley
format	Journal article
container_title	Parasitology
container_volume	147
container_issue	11
container_start_page	1229
publishDate	2020
institution	Swansea University
issn	0031-1820 1469-8161
doi_str_mv	10.1017/s0031182020000980
publisher	Cambridge University Press (CUP)
college_str	Faculty of Science and Engineering
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department_str	School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
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description	This study provides a morphological and phylogenetic characterization of two novel species of the order Haplosporida (Haplosporidium carcini n. sp., and H. cranc n. sp.) infecting the common shore crab Carcinus maenas collected at one location in Swansea Bay, South Wales, UK. Both parasites were observed in the haemolymph, gills and hepatopancreas. The prevalence of clinical infections (i.e. parasites seen directly in fresh haemolymph preparations) was low, at ~1%, whereas subclinical levels, detected by polymerase chain reaction, were slightly higher at ~2%. Although no spores were found in any of the infected crabs examined histologically (n = 334), the morphology of monokaryotic and dikaryotic unicellular stages of the parasites enabled differentiation between the two new species. Phylogenetic analyses of the new species based on the small subunit (SSU) rDNA gene placed H. cranc in a clade of otherwise uncharacterized environmental sequences from marine samples, and H. carcini in a clade with other crustacean-associated lineages.
published_date	2020-09-01T04:07:57Z
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Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.

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