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Uterine infusion of bacteria alters the transcriptome of bovine oocytes

Rachel L. Piersanti, Jeremy Block, Zhengxin Ma, KwangCheol C. Jeong, José E. P. Santos, Fahong Yu, Martin Sheldon Orcid Logo, John J. Bromfield

FASEB BioAdvances, Volume: 2, Issue: 8, Pages: 506 - 520

Swansea University Author: Martin Sheldon Orcid Logo

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DOI (Published version): 10.1096/fba.2020-00029

Abstract

Postpartum uterine infection reduces fertility in dairy cattle; however, the mechanisms of uterine infection‐mediated infertility are unknown. Paradoxically, infection‐induced infertility persists after the resolution of disease. Oocytes are a finite resource, which are present at various stages of...

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Published in: FASEB BioAdvances
ISSN: 2573-9832 2573-9832
Published: Wiley 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa54685
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spelling 2020-08-28T11:25:34.4877750 v2 54685 2020-07-10 Uterine infusion of bacteria alters the transcriptome of bovine oocytes ab0f74b794e59cc270c69e63ee1d9748 0000-0001-7902-5558 Martin Sheldon Martin Sheldon true false 2020-07-10 BMS Postpartum uterine infection reduces fertility in dairy cattle; however, the mechanisms of uterine infection‐mediated infertility are unknown. Paradoxically, infection‐induced infertility persists after the resolution of disease. Oocytes are a finite resource, which are present at various stages of development during uterine infection. It is likely that oocyte development is influenced by uterine infection‐induced changes to the follicular microenvironment. To better understand the impact of infection on oocyte quality we employed global transcriptomics of oocytes collected from heifers after receiving intrauterine infusion of pathogenic Escherichia coli and Trueperella pyogenes . We hypothesized that the oocyte transcriptome would be altered in response to intrauterine infection. A total of 452 differentially expressed genes were identified in oocytes collected from heifers 4 days after bacteria infusion compared to vehicle infusion, while 539 differentially expressed genes were identified in oocytes collected from heifers 60 days after bacteria infusion. Only 42 genes were differentially expressed in bacteria‐infused heifers at both Day 4 and Day 60. Interferon, HMGB1, ILK, IL‐6, and TGF‐beta signaling pathways were downregulated in oocytes collected at Day 4 from bacteria‐infused heifers, while interferon, ILK, and IL‐6 signaling were upregulated in oocytes collected at Day 60 from bacteria‐infused heifers. These data suggest that bacterial infusion alters the oocyte transcriptome differently at Day 4 and Day 60, suggesting different follicle stages are susceptible to damage. Characterizing the long‐term impacts of uterine infection on the oocyte transcriptome aids in our understanding of how infection causes infertility in dairy cattle. Journal Article FASEB BioAdvances 2 8 506 520 Wiley 2573-9832 2573-9832 endometritis, gene expression, infection, inflammation 16 8 2020 2020-08-16 10.1096/fba.2020-00029 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University NIH 2020-08-28T11:25:34.4877750 2020-07-10T16:01:33.5902065 Rachel L. Piersanti 1 Jeremy Block 2 Zhengxin Ma 3 KwangCheol C. Jeong 4 José E. P. Santos 5 Fahong Yu 6 Martin Sheldon 0000-0001-7902-5558 7 John J. Bromfield 8 54685__17935__48411ecef0c849ef8f66bab9ffc5db47.pdf PiersantiFASEB2020.pdf 2020-08-17T08:08:38.4207814 Output 830559 application/pdf Version of Record true Released under the terms of a Creative Commons Attribution License (CC-BY). true eng http://creativecommons.org/licenses/by/4.0/
title Uterine infusion of bacteria alters the transcriptome of bovine oocytes
spellingShingle Uterine infusion of bacteria alters the transcriptome of bovine oocytes
Martin Sheldon
title_short Uterine infusion of bacteria alters the transcriptome of bovine oocytes
title_full Uterine infusion of bacteria alters the transcriptome of bovine oocytes
title_fullStr Uterine infusion of bacteria alters the transcriptome of bovine oocytes
title_full_unstemmed Uterine infusion of bacteria alters the transcriptome of bovine oocytes
title_sort Uterine infusion of bacteria alters the transcriptome of bovine oocytes
author_id_str_mv ab0f74b794e59cc270c69e63ee1d9748
author_id_fullname_str_mv ab0f74b794e59cc270c69e63ee1d9748_***_Martin Sheldon
author Martin Sheldon
author2 Rachel L. Piersanti
Jeremy Block
Zhengxin Ma
KwangCheol C. Jeong
José E. P. Santos
Fahong Yu
Martin Sheldon
John J. Bromfield
format Journal article
container_title FASEB BioAdvances
container_volume 2
container_issue 8
container_start_page 506
publishDate 2020
institution Swansea University
issn 2573-9832
2573-9832
doi_str_mv 10.1096/fba.2020-00029
publisher Wiley
document_store_str 1
active_str 0
description Postpartum uterine infection reduces fertility in dairy cattle; however, the mechanisms of uterine infection‐mediated infertility are unknown. Paradoxically, infection‐induced infertility persists after the resolution of disease. Oocytes are a finite resource, which are present at various stages of development during uterine infection. It is likely that oocyte development is influenced by uterine infection‐induced changes to the follicular microenvironment. To better understand the impact of infection on oocyte quality we employed global transcriptomics of oocytes collected from heifers after receiving intrauterine infusion of pathogenic Escherichia coli and Trueperella pyogenes . We hypothesized that the oocyte transcriptome would be altered in response to intrauterine infection. A total of 452 differentially expressed genes were identified in oocytes collected from heifers 4 days after bacteria infusion compared to vehicle infusion, while 539 differentially expressed genes were identified in oocytes collected from heifers 60 days after bacteria infusion. Only 42 genes were differentially expressed in bacteria‐infused heifers at both Day 4 and Day 60. Interferon, HMGB1, ILK, IL‐6, and TGF‐beta signaling pathways were downregulated in oocytes collected at Day 4 from bacteria‐infused heifers, while interferon, ILK, and IL‐6 signaling were upregulated in oocytes collected at Day 60 from bacteria‐infused heifers. These data suggest that bacterial infusion alters the oocyte transcriptome differently at Day 4 and Day 60, suggesting different follicle stages are susceptible to damage. Characterizing the long‐term impacts of uterine infection on the oocyte transcriptome aids in our understanding of how infection causes infertility in dairy cattle.
published_date 2020-08-16T04:08:22Z
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score 11.035874

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