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Molecular Binding of EuIII/CmIII by Stenotrophomonas bentonitica and Its Impact on the Safety of Future Geodisposal of Radioactive Waste / Miguel A. Ruiz-Fresneda, Margarita Lopez-Fernandez, Marcos F. Martinez-Moreno, Andrea Cherkouk, Yon Ju-Nam, Jesus Ojeda Ledo, Henry Moll, Mohamed L. Merroun
Environmental Science & Technology, Volume: 54, Issue: 23, Pages: 15180 - 15190
Accepted Manuscript under embargo until: 13th November 2021
Microbial communities occurring in reference materials for artificial barriers (e.g. bentonites) in future deep geological repositories of radioactive waste can influence the migration behavior of radionuclides such as curium (CmIII). This study investigates the molecular interactions between CmIII...
|Published in:||Environmental Science & Technology|
American Chemical Society (ACS)
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Microbial communities occurring in reference materials for artificial barriers (e.g. bentonites) in future deep geological repositories of radioactive waste can influence the migration behavior of radionuclides such as curium (CmIII). This study investigates the molecular interactions between CmIII and its inactive analogue europium (EuIII) with the indigenous bentonite bacterium Stenotrophomonas bentonitica at environmentally relevant concentrations. Potentiometric studies showed a remarkable high concentration of phosphates at the bacterial cell wall compared to other bacteria, revealing the great potential of S. bentonitica for metal binding. Infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the role of phosphates and carboxylate groups from the cell envelope in the bioassociation of EuIII. Additionally, time-resolved laser-induced fluorescence spectroscopy (TRLFS) identified phosphoryl and carboxyl groups from bacterial envelopes, among other released complexing agents, to be involved in the EuIII and CmIII coordination. The ability of this bacteria to form a biofilm at the surface of bentonites allow them to immobilize trivalent lanthanide and actinides in the environment.
europium; curium; bacterial speciation; mobility; geodisposal
College of Engineering