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RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection

Ekaterina Grizanova, Christopher Coates, Tariq Butt Orcid Logo, Ivan Dubovskiy

Developmental & Comparative Immunology, Volume: 122, Start page: 104126

Swansea University Authors: Ekaterina Grizanova, Christopher Coates, Tariq Butt Orcid Logo, Ivan Dubovskiy

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Abstract

The co-evolutionary arms race between disease-causing agents and their insect victims is ancient and complex – leading to the development of specialised attack and defence strategies. Among such strategies is the capacity of fungal and oomycete pathogens to deploy degradative enzymes, notably protea...

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Published in: Developmental & Comparative Immunology
ISSN: 0145-305X
Published: Elsevier BV 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa56776
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spelling 2021-08-16T09:52:46.2326957 v2 56776 2021-05-04 RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection fa72411568b7eed3c2e810929ef425a6 Ekaterina Grizanova Ekaterina Grizanova true false af160934b75bea5b8ba83d68b3d1a003 Christopher Coates Christopher Coates true false 85d1c2ddde272a1176e74978e25ebece 0000-0002-8789-9543 Tariq Butt Tariq Butt true false 411b6e5b2086d0ffc3e89b14127d2768 Ivan Dubovskiy Ivan Dubovskiy true false 2021-05-04 FGSEN The co-evolutionary arms race between disease-causing agents and their insect victims is ancient and complex – leading to the development of specialised attack and defence strategies. Among such strategies is the capacity of fungal and oomycete pathogens to deploy degradative enzymes, notably proteases, to facilitate infection directly across the integument. To counter these proteases, insects such as the greater wax moth Galleria mellonella release metalloprotease inhibitors and other immune factors to thwart the invading fungus. To date, molecular-based confirmation of insect metalloprotease inhibitor’s incontrovertible role in antifungal defence has been lacking. We targeted the IMPI gene for suppression using RNAi and exposed those insects to the entomopathogenic fungus Metarhizium brunneum ARSEF4556. Levels of IMPI were reduced significantly in the integument (10-fold) and fat body (5-fold) of RNAi-treated insects when compared to control larvae, and displayed a significantly higher mortality rate. We also surveyed candidate immune/detoxification gene expression levels (e.g., DOPA decarboxylase, galiomycin) in three tissues (integument, midgut, fat body) in order to gauge any potential non-target effects of RNAi. The loss of IMPI via RNAi compromises antifungal defences and leaves G. mellonella vulnerable to infection. Journal Article Developmental & Comparative Immunology 122 104126 Elsevier BV 0145-305X Entomopathogenic fungi; Non-target genes; RNA interference; Innate immunity; Detoxification; Antimicrobial peptides 1 9 2021 2021-09-01 10.1016/j.dci.2021.104126 COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University Not Required 2021-08-16T09:52:46.2326957 2021-05-04T12:17:38.1830490 College of Science Biosciences Ekaterina Grizanova 1 Christopher Coates 2 Tariq Butt 0000-0002-8789-9543 3 Ivan Dubovskiy 4
title RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection
spellingShingle RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection
Ekaterina Grizanova
Christopher Coates
Tariq Butt
Ivan Dubovskiy
title_short RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection
title_full RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection
title_fullStr RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection
title_full_unstemmed RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection
title_sort RNAi-mediated suppression of insect metalloprotease inhibitor (IMPI) enhances Galleria mellonella susceptibility to fungal infection
author_id_str_mv fa72411568b7eed3c2e810929ef425a6
af160934b75bea5b8ba83d68b3d1a003
85d1c2ddde272a1176e74978e25ebece
411b6e5b2086d0ffc3e89b14127d2768
author_id_fullname_str_mv fa72411568b7eed3c2e810929ef425a6_***_Ekaterina Grizanova
af160934b75bea5b8ba83d68b3d1a003_***_Christopher Coates
85d1c2ddde272a1176e74978e25ebece_***_Tariq Butt
411b6e5b2086d0ffc3e89b14127d2768_***_Ivan Dubovskiy
author Ekaterina Grizanova
Christopher Coates
Tariq Butt
Ivan Dubovskiy
author2 Ekaterina Grizanova
Christopher Coates
Tariq Butt
Ivan Dubovskiy
format Journal article
container_title Developmental & Comparative Immunology
container_volume 122
container_start_page 104126
publishDate 2021
institution Swansea University
issn 0145-305X
doi_str_mv 10.1016/j.dci.2021.104126
publisher Elsevier BV
college_str College of Science
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hierarchy_top_id collegeofscience
hierarchy_top_title College of Science
hierarchy_parent_id collegeofscience
hierarchy_parent_title College of Science
department_str Biosciences{{{_:::_}}}College of Science{{{_:::_}}}Biosciences
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description The co-evolutionary arms race between disease-causing agents and their insect victims is ancient and complex – leading to the development of specialised attack and defence strategies. Among such strategies is the capacity of fungal and oomycete pathogens to deploy degradative enzymes, notably proteases, to facilitate infection directly across the integument. To counter these proteases, insects such as the greater wax moth Galleria mellonella release metalloprotease inhibitors and other immune factors to thwart the invading fungus. To date, molecular-based confirmation of insect metalloprotease inhibitor’s incontrovertible role in antifungal defence has been lacking. We targeted the IMPI gene for suppression using RNAi and exposed those insects to the entomopathogenic fungus Metarhizium brunneum ARSEF4556. Levels of IMPI were reduced significantly in the integument (10-fold) and fat body (5-fold) of RNAi-treated insects when compared to control larvae, and displayed a significantly higher mortality rate. We also surveyed candidate immune/detoxification gene expression levels (e.g., DOPA decarboxylase, galiomycin) in three tissues (integument, midgut, fat body) in order to gauge any potential non-target effects of RNAi. The loss of IMPI via RNAi compromises antifungal defences and leaves G. mellonella vulnerable to infection.
published_date 2021-09-01T04:12:32Z
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