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Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature

Anna Rawlings Orcid Logo, Eoin O'Connor, Suzy Moody, Ed Dudley, Lynne Boddy, Mike Fowler Orcid Logo, David A. Fitzpatrick, Sean Doyle, Dan Eastwood Orcid Logo

Journal of Ecology, Volume: 110, Issue: 1, Pages: 68 - 79

Swansea University Authors: Anna Rawlings Orcid Logo, Suzy Moody, Ed Dudley, Mike Fowler Orcid Logo, Dan Eastwood Orcid Logo

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Abstract

Decomposition of lignin-rich wood by fungi drives nutrient recycling in woodland ecosystems. Fluctuating abiotic conditions are known to promote the functioning of ecological communities and ecosystems. In the context of wood decay, fluctuating temperature increases decomposition rates. Metabolomics...

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Published in: Journal of Ecology
ISSN: 0022-0477 1365-2745
Published: Wiley 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa57065
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Fluctuating abiotic conditions are known to promote the functioning of ecological communities and ecosystems. In the context of wood decay, fluctuating temperature increases decomposition rates. Metabolomics, in tandem with other &#x2018;omics tools, can highlight the metabolic processes affected by experimental treatments, even in the absence of genome sequences and annotations. Globally, natural wood decay communities are dominated by the phylum Basidiomycota. We examined the metabolic responses of Mucidula mucida, a dominant constituent of pioneer communities in beech branches in British woodlands, and Exidia glandulosa, a stress-selected constituent of the same communities, in response to constant and diurnally cycling temperature. We applied untargeted metabolomics and proteomics to beech wood blocks, colonised by M. mucida or E. glandulosa and exposed to either diurnally cycling (mean 15 &#xB1; 10&#xB0;C) or constant (15&#xB0;C) temperature, in a fully factorial design. Metabolites and proteins linked to lignin breakdown, the citric acid cycle, pentose phosphate pathway, carbohydrate metabolism, fatty acid metabolism and protein biosynthesis and turnover were under-enriched in fluctuating, compared to stable temperatures, in the generalist M. mucida. Conversely, E. glandulosa showed little differential response to the experimental treatments. Synthesis. By demonstrating temperature-dependant metabolic signatures related to nutrient acquisition in a generalist wood decay fungus, we provide new insights into how abiotic conditions can affect community-mediated decomposition and carbon turnover in forests. 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spelling 2022-01-13T13:33:04.6185000 v2 57065 2021-06-08 Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature 46366e9ea4e8840d8c216e76c2f85204 NULL Anna Rawlings Anna Rawlings true true 37a093368072d1b157efd45c8c219c26 Suzy Moody Suzy Moody true false c7d05f992a817cd3b9a5f946bd909b71 Ed Dudley Ed Dudley true false a3a29027498d4b43a3f082a0a5ba16b4 0000-0003-1544-0407 Mike Fowler Mike Fowler true false 4982f3fa83886c0362e2bb43ce1c027f 0000-0002-7015-0739 Dan Eastwood Dan Eastwood true false 2021-06-08 Decomposition of lignin-rich wood by fungi drives nutrient recycling in woodland ecosystems. Fluctuating abiotic conditions are known to promote the functioning of ecological communities and ecosystems. In the context of wood decay, fluctuating temperature increases decomposition rates. Metabolomics, in tandem with other ‘omics tools, can highlight the metabolic processes affected by experimental treatments, even in the absence of genome sequences and annotations. Globally, natural wood decay communities are dominated by the phylum Basidiomycota. We examined the metabolic responses of Mucidula mucida, a dominant constituent of pioneer communities in beech branches in British woodlands, and Exidia glandulosa, a stress-selected constituent of the same communities, in response to constant and diurnally cycling temperature. We applied untargeted metabolomics and proteomics to beech wood blocks, colonised by M. mucida or E. glandulosa and exposed to either diurnally cycling (mean 15 ± 10°C) or constant (15°C) temperature, in a fully factorial design. Metabolites and proteins linked to lignin breakdown, the citric acid cycle, pentose phosphate pathway, carbohydrate metabolism, fatty acid metabolism and protein biosynthesis and turnover were under-enriched in fluctuating, compared to stable temperatures, in the generalist M. mucida. Conversely, E. glandulosa showed little differential response to the experimental treatments. Synthesis. By demonstrating temperature-dependant metabolic signatures related to nutrient acquisition in a generalist wood decay fungus, we provide new insights into how abiotic conditions can affect community-mediated decomposition and carbon turnover in forests. We show that mechanisms underpinning important biogeochemical processes can be highlighted using untargeted metabolomics and proteomics in the absence of well-annotated genomes. Journal Article Journal of Ecology 110 1 68 79 Wiley 0022-0477 1365-2745 diurnal cycles; ligninolytic fungi; metabolomics; proteomics; temperature; wood decay 12 1 2022 2022-01-12 10.1111/1365-2745.13716 COLLEGE NANME Biosciences COLLEGE CODE Swansea University SU Library paid the OA fee (TA Institutional Deal) Science Foundation Ireland. Grant Number: SFI 12/RI/2346(3); Natural Environment Research Council. Grant Numbers: NE/K011383/1, NE/K011588/1 2022-01-13T13:33:04.6185000 2021-06-08T16:15:50.8605423 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Anna Rawlings NULL 1 Eoin O'Connor 2 Suzy Moody 3 Ed Dudley 4 Lynne Boddy 5 Mike Fowler 0000-0003-1544-0407 6 David A. Fitzpatrick 7 Sean Doyle 8 Dan Eastwood 0000-0002-7015-0739 9 57065__20609__1c1e0507e0ed4078a3428a3ca00eaa3e.pdf 57065.pdf 2021-08-11T12:06:29.7188909 Output 1447711 application/pdf Version of Record true © 2021 The Authors. This is an open access article under the terms of the Creative Commons Attribution License true eng https://creativecommons.org/licenses/by/4.0/
title Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature
spellingShingle Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature
Anna Rawlings
Suzy Moody
Ed Dudley
Mike Fowler
Dan Eastwood
title_short Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature
title_full Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature
title_fullStr Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature
title_full_unstemmed Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature
title_sort Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature
author_id_str_mv 46366e9ea4e8840d8c216e76c2f85204
37a093368072d1b157efd45c8c219c26
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a3a29027498d4b43a3f082a0a5ba16b4
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author_id_fullname_str_mv 46366e9ea4e8840d8c216e76c2f85204_***_Anna Rawlings
37a093368072d1b157efd45c8c219c26_***_Suzy Moody
c7d05f992a817cd3b9a5f946bd909b71_***_Ed Dudley
a3a29027498d4b43a3f082a0a5ba16b4_***_Mike Fowler
4982f3fa83886c0362e2bb43ce1c027f_***_Dan Eastwood
author Anna Rawlings
Suzy Moody
Ed Dudley
Mike Fowler
Dan Eastwood
author2 Anna Rawlings
Eoin O'Connor
Suzy Moody
Ed Dudley
Lynne Boddy
Mike Fowler
David A. Fitzpatrick
Sean Doyle
Dan Eastwood
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container_title Journal of Ecology
container_volume 110
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publishDate 2022
institution Swansea University
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1365-2745
doi_str_mv 10.1111/1365-2745.13716
publisher Wiley
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hierarchy_parent_id facultyofscienceandengineering
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department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
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description Decomposition of lignin-rich wood by fungi drives nutrient recycling in woodland ecosystems. Fluctuating abiotic conditions are known to promote the functioning of ecological communities and ecosystems. In the context of wood decay, fluctuating temperature increases decomposition rates. Metabolomics, in tandem with other ‘omics tools, can highlight the metabolic processes affected by experimental treatments, even in the absence of genome sequences and annotations. Globally, natural wood decay communities are dominated by the phylum Basidiomycota. We examined the metabolic responses of Mucidula mucida, a dominant constituent of pioneer communities in beech branches in British woodlands, and Exidia glandulosa, a stress-selected constituent of the same communities, in response to constant and diurnally cycling temperature. We applied untargeted metabolomics and proteomics to beech wood blocks, colonised by M. mucida or E. glandulosa and exposed to either diurnally cycling (mean 15 ± 10°C) or constant (15°C) temperature, in a fully factorial design. Metabolites and proteins linked to lignin breakdown, the citric acid cycle, pentose phosphate pathway, carbohydrate metabolism, fatty acid metabolism and protein biosynthesis and turnover were under-enriched in fluctuating, compared to stable temperatures, in the generalist M. mucida. Conversely, E. glandulosa showed little differential response to the experimental treatments. Synthesis. By demonstrating temperature-dependant metabolic signatures related to nutrient acquisition in a generalist wood decay fungus, we provide new insights into how abiotic conditions can affect community-mediated decomposition and carbon turnover in forests. We show that mechanisms underpinning important biogeochemical processes can be highlighted using untargeted metabolomics and proteomics in the absence of well-annotated genomes.
published_date 2022-01-12T04:12:31Z
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