Journal article 455 views
Hemodilution Increases the Susceptibility of Red Blood Cells to Mechanical Shear Stress During In Vitro Hemolysis Testing
ASAIO Journal, Volume: 67, Issue: 6, Pages: 632 - 641
Swansea University Authors:
Christian Sargent, Venkat Kanamarlapudi
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DOI (Published version): 10.1097/mat.0000000000001280
Abstract
The American Society for Testing and Materials (ASTM) F1841 standard for the assessment of hemolysis in blood pumps recommends using phosphate-buffered saline (PBS) for hemodilution to standardize hematocrit (HCT). However, PBS increases red blood cell mechanical fragility and hemolysis. Herein, we...
Published in: | ASAIO Journal |
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ISSN: | 1058-2916 |
Published: |
Ovid Technologies (Wolters Kluwer Health)
2021
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URI: | https://cronfa.swan.ac.uk/Record/cronfa57577 |
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2022-10-27T11:21:57.5875420 v2 57577 2021-08-10 Hemodilution Increases the Susceptibility of Red Blood Cells to Mechanical Shear Stress During In Vitro Hemolysis Testing 949eccfb07970edf2941844b67a29d1a Christian Sargent Christian Sargent true false 63741801137148abfa4c00cd547dcdfa 0000-0002-8739-1483 Venkat Kanamarlapudi Venkat Kanamarlapudi true false 2021-08-10 The American Society for Testing and Materials (ASTM) F1841 standard for the assessment of hemolysis in blood pumps recommends using phosphate-buffered saline (PBS) for hemodilution to standardize hematocrit (HCT). However, PBS increases red blood cell mechanical fragility and hemolysis. Herein, we investigated diluents and dilutions during in vitro testing to reduce hemodilution bias when assessing hemolysis. Bovine blood was diluted with either PBS or PBS + 4/6 g% bovine serum albumin (BSA) to a 70/90% blood dilution, or to an HCT of 30% ± 2%, and pumped with the CentriMag or RotaFlow under hemodynamic conditions. Separately, bovine and human blood were subjected to ventricular assist device–like shear stress using a vortex. Plasma-free hemoglobin levels, normalized milligram index of hemolysis (mgNIH), and protein concentrations were analyzed. Hemolysis depended on the diluent and final blood concentration. Seventy percent of blood diluted with PBS alone caused significantly greater hemolysis than PBS + 4/6 g% BSA. However, at 90% blood, PBS + 4/6 g% BSA caused significantly greater hemolysis than PBS alone. Hence, a positive correlation between mgNIH and hemodilution was observed with PBS and a negative correlation with PBS + 4g% BSA. PBS alone significantly reduced the total protein concentration. Hemodilution with BSA maintains protein concentration within a physiologic range and reduces bias during hemolysis testing at high blood dilutions. Thus, American Society for Testing and Materials standards could consider including BSA as a diluent, when and as required: where large dilution is required (<83%) use PBS + 4 g% BSA, otherwise use PBS alone. Journal Article ASAIO Journal 67 6 632 641 Ovid Technologies (Wolters Kluwer Health) 1058-2916 1 6 2021 2021-06-01 10.1097/mat.0000000000001280 COLLEGE NANME COLLEGE CODE Swansea University 2022-10-27T11:21:57.5875420 2021-08-10T11:29:58.9911415 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Christian Sargent 1 Ina Laura Perkins 2 Venkat Kanamarlapudi 0000-0002-8739-1483 3 Christopher Moriarty 4 Sabrina Ali 5 |
title |
Hemodilution Increases the Susceptibility of Red Blood Cells to Mechanical Shear Stress During In Vitro Hemolysis Testing |
spellingShingle |
Hemodilution Increases the Susceptibility of Red Blood Cells to Mechanical Shear Stress During In Vitro Hemolysis Testing Christian Sargent Venkat Kanamarlapudi |
title_short |
Hemodilution Increases the Susceptibility of Red Blood Cells to Mechanical Shear Stress During In Vitro Hemolysis Testing |
title_full |
Hemodilution Increases the Susceptibility of Red Blood Cells to Mechanical Shear Stress During In Vitro Hemolysis Testing |
title_fullStr |
Hemodilution Increases the Susceptibility of Red Blood Cells to Mechanical Shear Stress During In Vitro Hemolysis Testing |
title_full_unstemmed |
Hemodilution Increases the Susceptibility of Red Blood Cells to Mechanical Shear Stress During In Vitro Hemolysis Testing |
title_sort |
Hemodilution Increases the Susceptibility of Red Blood Cells to Mechanical Shear Stress During In Vitro Hemolysis Testing |
author_id_str_mv |
949eccfb07970edf2941844b67a29d1a 63741801137148abfa4c00cd547dcdfa |
author_id_fullname_str_mv |
949eccfb07970edf2941844b67a29d1a_***_Christian Sargent 63741801137148abfa4c00cd547dcdfa_***_Venkat Kanamarlapudi |
author |
Christian Sargent Venkat Kanamarlapudi |
author2 |
Christian Sargent Ina Laura Perkins Venkat Kanamarlapudi Christopher Moriarty Sabrina Ali |
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ASAIO Journal |
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67 |
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632 |
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2021 |
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Swansea University |
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1058-2916 |
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10.1097/mat.0000000000001280 |
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Ovid Technologies (Wolters Kluwer Health) |
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Faculty of Medicine, Health and Life Sciences |
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Faculty of Medicine, Health and Life Sciences |
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Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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description |
The American Society for Testing and Materials (ASTM) F1841 standard for the assessment of hemolysis in blood pumps recommends using phosphate-buffered saline (PBS) for hemodilution to standardize hematocrit (HCT). However, PBS increases red blood cell mechanical fragility and hemolysis. Herein, we investigated diluents and dilutions during in vitro testing to reduce hemodilution bias when assessing hemolysis. Bovine blood was diluted with either PBS or PBS + 4/6 g% bovine serum albumin (BSA) to a 70/90% blood dilution, or to an HCT of 30% ± 2%, and pumped with the CentriMag or RotaFlow under hemodynamic conditions. Separately, bovine and human blood were subjected to ventricular assist device–like shear stress using a vortex. Plasma-free hemoglobin levels, normalized milligram index of hemolysis (mgNIH), and protein concentrations were analyzed. Hemolysis depended on the diluent and final blood concentration. Seventy percent of blood diluted with PBS alone caused significantly greater hemolysis than PBS + 4/6 g% BSA. However, at 90% blood, PBS + 4/6 g% BSA caused significantly greater hemolysis than PBS alone. Hence, a positive correlation between mgNIH and hemodilution was observed with PBS and a negative correlation with PBS + 4g% BSA. PBS alone significantly reduced the total protein concentration. Hemodilution with BSA maintains protein concentration within a physiologic range and reduces bias during hemolysis testing at high blood dilutions. Thus, American Society for Testing and Materials standards could consider including BSA as a diluent, when and as required: where large dilution is required (<83%) use PBS + 4 g% BSA, otherwise use PBS alone. |
published_date |
2021-06-01T04:13:26Z |
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1763753911782473728 |
score |
11.021648 |